Three-dimensional cell-adhesive matrix of silk cocoon derived carbon fiber assembled with iron-porphyrin for monitoring cell released signal molecules

2021 ◽  
Vol 334 ◽  
pp. 129594
Author(s):  
Fang Xin Hu ◽  
Xiaoli Xie ◽  
Dongping Wang ◽  
Hong Bin Yang ◽  
Yu Gu ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Three Dimensional ◽  
Iron Porphyrin ◽  
Signal Molecules ◽  
Silk Cocoon ◽  
Adhesive Matrix ◽  
Cell Adhesive ◽  
Dimensional Cell

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

Three-Dimensional (3D) Nanostructured Skeleton Substrate Composed of Hollow Carbon Fiber/Carbon Nanosheet/ZnO for Stable Lithium Anode

2021 ◽  
Author(s):  
Xue-Liang Zhang ◽  
Zhi-Qin Ruan ◽  
Qiao-Tong He ◽  
Xu-Jia Hong ◽  
Xin Song ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Three Dimensional ◽  
Lithium Anode ◽  
Hollow Carbon

Bending response of carbon fiber composite sandwich beams with three dimensional honeycomb cores

2014 ◽  
Vol 108 ◽  
pp. 234-242 ◽  
Author(s):  
Jian Xiong ◽  
Li Ma ◽  
Ariel Stocchi ◽  
Jinshui Yang ◽  
Linzhi Wu ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Fiber Composite ◽  
Three Dimensional ◽  
Sandwich Beams ◽  
Carbon Fiber Composite ◽  
Composite Sandwich ◽  
Honeycomb Cores ◽  
Bending Response

Direct laser writing and geometrical analysis of scaffolds with designed pore architecture for three-dimensional cell culturing

2012 ◽  
Vol 22 (11) ◽  
pp. 115016 ◽  
Author(s):  
Elli Käpylä ◽  
Dogu Baran Aydogan ◽  
Sanni Virjula ◽  
Sari Vanhatupa ◽  
Susanna Miettinen ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Direct Laser Writing ◽  
Geometrical Analysis ◽  
Laser Writing ◽  
Cell Culturing ◽  
Pore Architecture ◽  
Direct Laser ◽  
Dimensional Cell

scholarly journals Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment

2017 ◽  
Vol 117 (20) ◽  
pp. 12764-12850 ◽  
Author(s):  
Guoyou Huang ◽  
Fei Li ◽  
Xin Zhao ◽  
Yufei Ma ◽  
Yuhui Li ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Biomimetic Materials ◽  
Cell Microenvironment ◽  
Dimensional Cell
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