Extreme learning machines for weather-based modelling of silk cocoon production

In spite of the immense popularity and sheer power of the neural network models, their application in sericulture is still very much limited. With this backdrop, this study evaluates the suitability of neural network models in comparison with the linear regression models in predicting silk cocoon production of the selected six districts (Kolar, Chikballapur, Ramanagara, Chamarajanagar, Mandya and Mysuru) of Karnataka by utilising weather variables for ten consecutive years (2009-2018). As the weather variables are found to be correlated, principal components are obtained and fed into the linear (principal component regression) and non-linear models (back propagation-artificial neural network and extreme learning machine) as inputs. Outcomes emanated from this experiment have revealed the clear advantages of employing extreme learning machines (ELMs) for weather-based modelling of silk cocoon production. Application of ELM would be particularly useful, when the relation between production and its attributing characters is complex and non-linear.

Anti-Herpes Simplex Virus Efficacy of Silk Cocoon, Silkworm Pupa and Non-Sericin Extracts

Herpes simplex virus (HSV) infections are prevalent worldwide and are the cause of life- threatening diseases. Standard treatment with antiviral drugs, such as acyclovir, could prevent serious complications; however, resistance has been reported specifically among immunocompromised patients. Therefore, the development of an alternative approach is needed. The silk cocoon derived from silkworm, Bombyx mori, has been recognized for its broad-spectrum biological activity, including antiviral activity; however, its effects against HSV infection are unknown. In this study, we investigated the inhibitory effects of silk extracts derived from the cocoon shell, silk cocoon, silkworm pupa and non-sericin extract, on blocking HSV-1 and HSV-2 binding to host cells, resulting in the inhibition of the virus infection in Vero cells. Non-sericin extract demonstrated the greatest effectiveness on inhibiting HSV-1 and HSV-2 binding activity. Moreover, the virucidal effect to inactivate HSV-1 and HSV-2 was determined and revealed that non-sericin extract also exerted the highest potential activity. Using the treatment of non-sericin extract in HSV-2-infected HeLa cells could significantly lower the HSV-induced cell death and prevent inflammation via lowering the inflammatory cytokine gene expression. The non-sericin extract was analyzed for its bioactive compounds in which gallic acid, flavonoid and xanthophyll were identified, and might have partially contributed to its antiviral activity. The finding in our study suggested the potential of silk extract as an alternative therapeutic treatment for HSV infection.

The Role of Filippi’s Glands in the Silk Moths Cocoon Construction

Filippi’s glands (FGs), formerly also called Lyonet’s glands, are accessory secretory structures of the labial (silk) glands of lepidopteran caterpillars, which were implicated to play an important role in the maturation of the silk material and the construction of the cocoon. In our previous study, we have identified several species of giant silk moths that completely lack the FGs. Interestingly, the absence of FGs in these species correlates with the construction of a loose cocoon architecture. We investigated the functions of FGs by their surgical extirpation in the last instar larvae of the silkworm, Bombyx mori. We found that the absence of FGs altered the structure of the resulting cocoon, in which the different layers of silk were separated. In further experiments, we found no effects of the absence of FGs on larval cocoon formation behavior or on changes in cocoon mass or lipid content. Differential proteomic analysis revealed no significant contribution of structural proteins from FGs to silk cocoon material, but we identified several low abundance proteins that may play a role in posttranslational modifications of some silk proteins. Proteomic analysis also revealed a difference in phosphorylation of the N-terminal sequence of fibroin-heavy chain molecule. Thus, FGs appear to affect silk stickiness during spinning by regulating posttranslational modifications. This could also explain the link that exists between the absence of these glands and the formation of loose cocoons in some giant silk moth species.

Research of quality silk cocoon making technology

This article presents the period of wrapping a silkworm in a cocoon during the season of making cocoons, as well as the results of experiments on their collection and analysis, existing problems. Based on the results, proposals were made to improve the system of attracting the population to entrepreneurship and the development of entrepreneurship. In particular, the effectiveness of organizing capacities for the production of raw silk by cocooning a silkworm at home, serving the enterprises of the silk cluster, is explained.

The Filippi’s Glands of Giant Silk Moths: To Be or Not to Be?

The Filippi’s glands (FGs), formerly “Lyonet’s glands”, are paired accessory organs associated with the silk glands. They are unique to Lepidoptera caterpillars and their exact role is not clear. The FGs are thought to be involved in the construction of a silk cocoon in bombycoid moths. FGs can differ in size and shape, therefore, in this study we attempt to find a correlation between FG morphology and phylogenetic position within the Bombycoidea. We use light and electron microscopy to examine the presence and morphology of FGs in a range of wild (giant) silk moths and several related species. Our results confirm that the majority of studied silk moth species have complex type of FGs that continuously increase in size during larval development. We identified several species of giant silk moths and two hawk moth species that completely lack FGs throughout their larval development. Finally, in several hawk moth species in which FGs are well developed during the first larval stage, these glands do not grow and remain small during later larval growth. Our results suggest that FGs are not critical for spinning and that loss of FGs occurred several times during the evolution of saturniids and sphingids. Comparison of FGs in different moths is an important first step in the elucidation of their physiological significance.

Silk Cocoon Production in Dharmapuri District of Tamil Nadu: A Profitability Analysis

Sericulture is a financially viable production and has enormous potential for employment opportunities. The present study attempted to investigate the profitability of the sericulture business in the Dharmapuri district of Tamil Nadu. A proportionate random sampling approach was used to select 80 sample sericulture farmers. The results revealed that the total cost of producing silk cocoons was ?98,805.1 , with a net profit of ? 1,17,194.84. The benefit-cost ratio and break-even output estimated 6 were 2.19:1 and 140.72 kg of the silk cocoon. It depicted that the farmers were operating in the profit zone, as the average yield of sample farmers was substantially higher. Labour scarcity, pest and disease incidence, and income loss owing to COVID 19 pandemic were major constraints faced by sericulture farmers. Sericulture enterprise provided immense opportunity by means of profitable income and more employment opportunities. The young unemployed rural entrepreneurs and women SHGs of this region if appropriately trained and provided financial support to initiate this enterprise would lead to the development of the silk cluster and indeed top position in the Indian silk industry.

Functionality of Silk Cocoon (Bombyx mori L.) Sericin Extracts Obtained through High-Temperature Hydrothermal Method

Sericin, a textile waste, can be used for antioxidant and skin-whitening purposes. The hydrothermal method of extracting sericin is more eco-friendly than are chemical and enzymatic methods. In this study, silk cocoons were cut into pieces and then subjected to hydrothermal extraction at three temperatures (160, 200, and 220 °C) to obtain sericin extracts (Sericin160, Sericin200, and Sericin220, respectively). Antioxidant activity and tyrosinase inhibition were measured to determine the extracts’ effectiveness. Sericin220 was the strongest antioxidant, with total phenol content, total flavonoid content, and ferric reducing power of 62.19 ± 0.04 mg gallic acid equivalents/g dry weight, 0.07 ± 0.01 mg quercetin equivalent/g dry weight, and 181.49 ± 0.024 mg vitamin C equivalent/g dry weight, respectively. The half-maximal inhibitory concentrations for DPPH and ABTS free-radical scavenging ability were 6.41 ± 0.05 and 0.79 ± 0.37 mg/mL, respectively. Sericin220 also exhibited the highest tyrosinase inhibition activity (70.82 ± 4.1 mg vitamin C equivalent/g), indicating its whitening potential.