Establishment of reference intervals for serum concentrations of androstanediol glucuronide by a newly developed LC-MS/MS method

Steroids ◽  
2019 ◽  
Vol 143 ◽  
pp. 62-66 ◽  
Author(s):  
Neus Fabregat-Cabello ◽  
Stéphanie D. Peeters ◽  
Tugba Yilmaz ◽  
Étienne Cavalier ◽  
Caroline M. Le Goff
Keyword(s):  
Reference Intervals ◽  
Serum Concentrations ◽  
Androstanediol Glucuronide

Validation of commercial assays for measurements of trefoil factor family peptides in serum

2011 ◽  
Vol 49 (12) ◽  
Author(s):  
Mie H. Samson ◽  
Ebba Nexo
Keyword(s):  
Blood Donors ◽  
Reference Intervals ◽  
Cross Reactivity ◽  
Serum Concentrations ◽  
Trefoil Factor ◽  
Serum Samples ◽  
Measuring Range ◽  
Trefoil Peptides ◽  
Pathological Conditions ◽  
Trefoil Factor Family

AbstractTrefoil peptides (TFF1, TFF2 and TFF3) are 7–12 kDa molecules, secreted by mucin-producing epithelial cells. Increased serum concentrations have been reported in a number of pathological conditions, which warrants the need for validated commercially available assays.We validated commercial assays for TFF1-3 and compared results obtained with our in-house assays, using serum from blood donors.Level of detection was: ≤0.008 nmol/L. Measuring ranges were: 0.032–0.51 (TFF1), 0.038–0.76 (TFF2) and 0.019–0.15 (TFF3) nmol/L. Imprecision (CV), judged from the measurement of serum pools in two levels, was below 9% (TFF2 and TFF3) but up to 18% (mean 0.41 nmol/L) for TFF1. No cross reactivity between the TFFs (concentrations >100 nmol/L) was observed. The 95% non-parametric reference intervals were: <0.0032–0.53 (TFF1), 0.099–1.4 (TFF2) and 0.086–0.87 (TFF3) nmol/L. Comparing commercial to in-house assays (n=132), showed biases explained by differences in the calibrators (TFF1 and TFF2). A number of samples showed markedly different results.The commercial assays for TFF2 and TFF3 are acceptable for use on serum samples, while the TFF1 assay revealed a poor imprecision and a too narrow measuring range. Results obtained with the commercial and the in-house assays differed, partly because of differences in the calibrators employed.

Multicentre physiological reference intervals for serum concentrations of immunoglobulins A, G and M, complement C3c and C4 measured with Tina-Quant® reagents systems

2007 ◽  
Vol 45 (3) ◽  
Author(s):  
Xavier Fuentes-Arderiu ◽  
Eduardo Alonso-Gregorio ◽  
Virtudes Álvarez-Funes ◽  
Carmen Ambrós-Marigómez ◽  
Lluís Coca-Fábregas ◽  
...  
Keyword(s):  
Reference Intervals ◽  
Serum Concentrations

scholarly journals Measurement of the α1-proteinase inhibitor (α1-antitrypsin) of common marmoset and intestinal protein loss in wasting syndrome

2019 ◽  
Vol 39 (7) ◽  
Author(s):  
Kimie Niimi ◽  
Hiromasa Morishita ◽  
Masaya Usui ◽  
Reiko Ito ◽  
Shino Kurata ◽  
...  
Keyword(s):  
Proteinase Inhibitor ◽  
Common Marmoset ◽  
Reference Intervals ◽  
Detection Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Concentrations ◽  
Healthy Individuals ◽  
Protein Loss ◽  
Wasting Syndrome ◽  
Intestinal Protein

AbstractAlthough wasting marmoset syndrome (WMS) is one of the biggest problems facing captive marmoset colonies, the mechanisms underlying its pathogenesis remain unclear. In our clinical experience, it is difficult to cure WMS-affected marmosets with severe hypoalbuminemia. Thus, the mechanisms underlying hypoalbuminemia in WMS must be understood. In the present study, we investigated whether intestinal protein loss, a known reason for hypoalbuminemia, occurs in this disease. Fecal α1-proteinase inhibitor (α1-PI, also known as α1-antitrypsin) has been used to diagnose intestinal protein loss in other species. To develop an assay system for this protein, marmoset α1-PI was purified from plasma and antibodies against it were developed using the purified protein. Using the antibodies, a sandwich enzyme-linked immunosorbent assay (ELISA) to measure marmoset α1-PI was developed, and its detection sensitivity for fecal samples was ∼20-fold higher than that of a commercial kit for human α1-PI. From this ELISA, the reference intervals for serum and feces of healthy marmosets were 0.87–1.85 mg/ml and 0.53–395.58 μg/g, respectively. The average concentrations of α1-PI in serum and feces of seven WMS-affected marmosets were 1.17 mg/ml and 1357.58 μg/g, respectively. Although there were no significant differences in the serum concentrations between healthy and WMS-affected marmosets, the fecal concentrations were significantly higher in WMS-affected marmosets than in healthy individuals, suggesting that intestinal protein loss occurs in WMS. Intestinal protein loss of WMS-affected marmosets was significantly attenuated with treatment, suggesting that it is one of the mechanisms involved in the hypoalbuminemia observed in WMS.

Pediatric and Perinatal Reference Intervals for Immunoglobulin Light Chains Kappa and Lambda

1992 ◽  
Vol 38 (4) ◽  
pp. 548-550 ◽  
Author(s):  
K R Herkner ◽  
H Salzer ◽  
A Böck ◽  
A Mühl ◽  
T Tsaka ◽  
...  
Keyword(s):  
Light Chain ◽  
Reference Intervals ◽  
Linear Increase ◽  
Light Chains ◽  
Serum Concentrations ◽  
Immunoglobulin Light Chains ◽  
Adult Type ◽  
Mean Values ◽  
Age Related ◽  
Chain Analysis

Abstract In routine analysis for immunoglobulin light chains in pediatric diagnostics, the age-related reference intervals for serum kappa (kappa) and lambda (lambda) light chains were evaluated in 1543 healthy subjects (newborns to age 16 years, including 168 premature infants). Light-chain analysis was performed by rate nephelometry. IgG, IgA, and IgM were measured simultaneously, and heavy- and light-chain differences were calculated for control purposes. Results for IgG, IgA, and IgM generally agreed with reference intervals reported in the literature. kappa showed age-related changes comparable with changes in IgG concentrations, whereas lambda showed moderate fluctuations. The kappa/lambda ratio showed an almost linear increase with age, starting with 0.97 at four months and reaching the highest value of 2.21 at 15 years (mean values). Preterm infants presented with markedly low serum concentrations of IgG and corresponding light chains but with adult-type kappa/lambda ratios because of the maternal-origin IgG.

Reference intervals for serum concentrations of three bone turnover markers for men and women

Bone ◽  
2013 ◽  
Vol 57 (2) ◽  
pp. 399-404 ◽  
Author(s):  
J. Michelsen ◽  
H. Wallaschofski ◽  
N. Friedrich ◽  
C. Spielhagen ◽  
R. Rettig ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Bone Turnover Markers ◽  
Reference Intervals ◽  
Serum Concentrations ◽  
Men And Women ◽  
Turnover Markers

Reference intervals for plasma proteins: similarities and differences between adult Caucasian and Asian Indian males in Yorkshire, UK

2004 ◽  
Vol 42 (7) ◽  
Author(s):  
A. Myron Johnson ◽  
Per Hyltoft Petersen ◽  
John T. Whicher ◽  
Anders Carlström ◽  
Sheila MacLennan
Keyword(s):  
Asian Indian ◽  
Plasma Proteins ◽  
Geographical Area ◽  
Reference Intervals ◽  
Racial Groups ◽  
Serum Concentrations ◽  
Adult Males ◽  
Gaussian Distributions ◽  
Two Generations ◽  
Similarities And Differences

AbstractThe aim of this study was to investigate similarities and differences in the distribution of serum concentrations of nine proteins in two racial groups (Caucasian and Asian Indian) of adult males living in the same geographical area (Leeds, Bradford, UK) for at least two generations. This is part of a larger study to determine the need for separating reference intervals for racial and ethnic groups worldwide. The distributions of concentrations for all proteins evaluated in the Indians fit ln-Gaussian distributions, indicating probable homogeneity. However, for the Caucasians, the distributions for α

Reference Intervals for Eight Modified Nucleosides in Serum in a Healthy Population from Italy and the United States

1992 ◽  
Vol 38 (5) ◽  
pp. 671-677 ◽  
Author(s):  
F Pane ◽  
G Oriani ◽  
K C Kuo ◽  
C W Gehrke ◽  
F Salvatore ◽  
...  
Keyword(s):  
United States ◽  
Reference Values ◽  
The United States ◽  
Reference Intervals ◽  
Hplc Method ◽  
Statistical Procedure ◽  
Modified Nucleosides ◽  
Healthy Population ◽  
Serum Concentrations ◽  
Element Analysis

Abstract We used a recently devised HPLC method to quantify eight modified nucleosides, an emerging group of tumor markers, in human serum and then calculated their reference intervals in a healthy population from Italy and the United States. We used the statistical procedure of element analysis, which reveals the effects of chosen variables (in this case, nationality, sex, and age) on an analyte (here, modified nucleosides). Using element analysis, we calculated the exact weight of each variable on the reference values. We found that nationality has the greatest effect on the serum concentrations of all the modified nucleosides apart from pseudouridine, whereas sex significantly influences only the concentrations of 4-pyridone-3-carboxamide-N1-ribofuranoside, 1-methylinosine and N2,N2-dimethylguanosine; age affects only N2,N2-dimethylguanosine. Thus, the reference intervals of all the nucleosides except pseudouridine were calculated separately for Italians and Americans, and the reference values for 4-pyridone-3-carboxamide-N1-ribofuranoside, 1-methylinosine, and N2,N2-dimethylguanosine were calculated separately for men and women. Our data form the baseline for study of variations in serum concentrations of modified nucleosides in various pathophysiological conditions.

Serum reference intervals of homoarginine, ADMA, and SDMA in the Study of Health in Pomerania

2014 ◽  
Vol 52 (12) ◽  
Author(s):  
Dorothee Atzler ◽  
Edzard Schwedhelm ◽  
Matthias Nauck ◽  
Till Ittermann ◽  
Rainer H. Böger ◽  
...  
Keyword(s):  
At Risk ◽  
Risk Stratification ◽  
Serum Levels ◽  
Reference Intervals ◽  
Specific Reference ◽  
Reference Ranges ◽  
Serum Concentrations ◽  
Improve Risk Stratification ◽  
Patients At Risk ◽  
Age And Sex

AbstractLow circulating homoarginine as well as high levels of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) have been associated with impaired cardiovascular (CV) outcome and mortality in patients at risk and in the general population. The present study aimed to formulate reference intervals for serum homoarginine, ADMA, and SDMA to improve risk stratification between healthy individuals and individuals at risk.We determined age- and sex-specific reference intervals for homoarginine, ADMA, and SDMA in a subgroup of 1359 healthy participants (no diabetes mellitus, CV disease, increased blood pressure, elevated blood lipids, chronic kidney disease stadium III or IV, or a body mass index >30) of the Study of Health in Pomerania (SHIP) using quantile regression analyses. Homoarginine, ADMA, and SDMA serum concentrations were measured using liquid chromatography-tandem mass spectrometry.Median age of the investigated cohort was 36 (25th; 75th percentile 28; 47) years, with 62% women. Median serum concentrations of homoarginine, ADMA, and SDMA were 2.63 (2.08; 3.32) μmol/L, 0.64 (0.57; 0.73) μmol/L, and 0.43 (0.37; 0.49) μmol/L, respectively. Serum levels of homoarginine, ADMA, and SDMA showed material age- and sex-related differences (p<0.05 for all). Overall reference ranges were 1.41–5.00 and 1.20–5.53 μmol/L (2.5th; 97.5th percentile; for men and women, respectively) for homoarginine, 0.41–0.95 and 0.43–0.96 μmol/L for ADMA, and 0.30–0.67 and 0.27–0.63 μmol/L for SDMA.We formulated for the first time homoarginine, ADMA, and SDMA reference intervals in serum. These reference intervals might be useful for individual CV risk stratification.

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