Effects of garlic oil and two of its major organosulfur compounds, diallyl disulfide and diallyl trisulfide, on intestinal damage in rats injected with endotoxin

2006 ◽  
Vol 213 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Yi-Hsuan Chiang ◽  
Lin-Ni Jen ◽  
Hsiau-Yuan Su ◽  
Chong-Kuei Lii ◽  
Lee-Yan Sheen ◽  
...  
Keyword(s):  
Diallyl Disulfide ◽  
Organosulfur Compounds ◽  
Intestinal Damage ◽  
Diallyl Trisulfide ◽  
Garlic Oil

scholarly journals Comparison of the Main Bioactive Compounds and Antioxidant Activity from Garlic Water-soluble and Garlic Oil

2017 ◽  
Vol 3 (5) ◽  
pp. 20 ◽  
Author(s):  
Ardhia Deasy Rosita Dewi ◽  
Joni Kusnadi ◽  
Wen-Ling Shih
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Diallyl Disulfide ◽  
Diallyl Trisulfide ◽  
Liquid Chromatography Mass Spectrometry ◽  
Soluble Extract ◽  
Bioactive Constituents ◽  
Garlic Oil ◽  
Water Soluble Extract

<p class="Els-Abstract-text">Garlic is a natural source which has abundant organosulfur constituents. Garlic is divided into water-soluble organosulfur constituents mainly SAC (S-allylcystein), NAC (N-acetylcysteine) and oil soluble organosulfur constituents such as DATS (diallyl trisulfide), DADS (diallyl disulfide), DAS (diallyl sulfide). The aim of this research was to compare the bioactive constituents and antioxidant activity between garlic water-soluble and garlic oil. Garlic water-soluble constituents were identified by Liquid Chromatography–Mass Spectrometry (LC-MS) and five constituents were found, namely N-acetylcysteine (NAC), cysteinyl-alanine, phenol-2-2-benzoxazolyl and two unknown constituents. The GC-MS chromatogram also showed three main constituents present in garlic oil as diallyldisulphide (DADS), diallyltrisulphide (DATS) and D-limonene. Interestingly, garlic water-soluble extract had higher antioxidant activity 70 %  ± 0.02 % in comparison with garlic oil 58 % ± 0.07 %. This study conducts a novel preparation of garlic water-soluble for enhancing antioxidant properties on garlic novel preparation.</p><p> </p>

scholarly journals VALIDATION AND ANALYSIS OF DIALLYL DISULFIDE AND DIALLYL TRISULFIDE IN GARLIC (ALLIUM SATIVUM L.) USING GAS CHROMATOGRAPHY

2020 ◽  
pp. 163-166
Author(s):  
HARMITA HARMITA ◽  
HERMAN SURYADI ◽  
LIDWINA DEVIANI LIKASA
Keyword(s):  
Gas Chromatography ◽  
Allium Sativum ◽  
Limit Of Detection ◽  
Diallyl Disulfide ◽  
Diallyl Trisulfide ◽  
Ionization Detector ◽  
Limit Of Quantification ◽  
Flame Ionization ◽  
Precision Limit ◽  
Optimized Conditions

Objective: The purpose of this research was to optimize and validate a method for measuring the levels of diallyl disulfide (DADS) and diallyl trisulfide(DATS) in garlic and single clove garlic.Methods: The analysis was performed using gas chromatography (GC) equipped with an HP-1 column and a flame ionization detector. The initialcolumn temperature was set at 140°C and increased at 1°C/min to 180°C. The injector and detector temperatures were set to 200°C, the carrier gasflow rate was 0.80 mL/min, and the injection volume was 1.0 μL. The optimized conditions of analysis were then validated which included selectivity,linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ).Results: Using the validated assay and a concentration range of 0.5–20 μg/mL, the coefficient of correlation (r) for DADS was 0.9999 and the LODand LOQ for DADS were 0.3063 μg/mL and 1.0210 μg/mL, respectively. Using the validated assay and a concentration range of 0.5–20 μg/mL, thecoefficient of correlation for DATS was 0.9999 and the LOD and LOQ for DATS were 0.1986 μg/mL and 0.6621 μg/mL, respectively. The percentage ofrecovery was in the range of 98.05–101.76% and coefficient of variation ≤ 2%.Conclusion: This GC method accurately measures the levels of DADS and DATS in garlic.

Radioprotective Activity of Naturally Occurring Organosulfur Compounds

2006 ◽  
Vol 92 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Manesh Chittezhath ◽  
Girija Kuttan
Keyword(s):  
Intestinal Mucosa ◽  
Lipid Peroxides ◽  
Allyl Isothiocyanate ◽  
Sulfur Compounds ◽  
Whole Body ◽  
Phenyl Isothiocyanate ◽  
Histopathological Analysis ◽  
Diallyl Disulfide ◽  
Organosulfur Compounds ◽  
Naturally Occurring

The radioprotective effects of naturally occurring sulfur compounds and isothiocyanates such as diallyl sulfide (DAS), diallyl disulfide (DADS), allyl methyl sulfide (AMS), allyl isothiocyanate (AITC) and phenyl isothiocyanate (PITC) have been investigated in whole body irradiated Swiss albino mice. Administration of these sulfur compounds could reduce the serum content of alkaline phosphatase (ALP), which was elevated after irradiation (23.9 ± 1.82 KA units). The elevated liver content of glutamate pyruvate transaminase (GPT) in control animals (76.2 ± 2.2 U/mL) after irradiation was significantly reduced in DAS (58.93 ± 4 U/mL) and AMS (55.7 ± 2.2 U/mL) treated animals. Elevated levels of lipid peroxides in serum and liver of irradiated control animals were also significantly reduced by treatment with these sulfur compounds. The glutathione (GSH) content in liver and intestinal mucosa was drastically reduced after irradiation. All the sulfur compounds and isothiocyanates could effectively enhance the GSH content of intestinal mucosa and liver. Findings at histopathological analysis of the intestine proved to be correlated with the above results.

scholarly journals Effects of organosulfur compounds from garlic oil on the antioxidation system in rat liver and red blood cells

2001 ◽  
Vol 39 (6) ◽  
pp. 563-569 ◽  
Author(s):  
C.C Wu ◽  
L.Y Sheen ◽  
H.-W Chen ◽  
S.-J Tsai ◽  
C.-K Lii
Keyword(s):  
Red Blood Cells ◽  
Rat Liver ◽  
Blood Cells ◽  
Organosulfur Compounds ◽  
Garlic Oil
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