Ability of garlic-derived diallyl disulfide and diallyl trisulfide supplemented by oral gavage to mitigate effects of an acute postweaning feed and water deprivation event in nursery pigs1

2017 ◽  
Vol 95 (8) ◽  
pp. 3579-3590 ◽  
Author(s):  
N. Horn ◽  
G. Miller ◽  
K. M. Ajuwon ◽  
O. Adeola
Keyword(s):  
Water Deprivation ◽  
Diallyl Disulfide ◽  
Diallyl Trisulfide ◽  
Oral Gavage

scholarly journals VALIDATION AND ANALYSIS OF DIALLYL DISULFIDE AND DIALLYL TRISULFIDE IN GARLIC (ALLIUM SATIVUM L.) USING GAS CHROMATOGRAPHY

2020 ◽  
pp. 163-166
Author(s):  
HARMITA HARMITA ◽  
HERMAN SURYADI ◽  
LIDWINA DEVIANI LIKASA
Keyword(s):  
Gas Chromatography ◽  
Allium Sativum ◽  
Limit Of Detection ◽  
Diallyl Disulfide ◽  
Diallyl Trisulfide ◽  
Ionization Detector ◽  
Limit Of Quantification ◽  
Flame Ionization ◽  
Precision Limit ◽  
Optimized Conditions

Objective: The purpose of this research was to optimize and validate a method for measuring the levels of diallyl disulfide (DADS) and diallyl trisulfide(DATS) in garlic and single clove garlic.Methods: The analysis was performed using gas chromatography (GC) equipped with an HP-1 column and a flame ionization detector. The initialcolumn temperature was set at 140°C and increased at 1°C/min to 180°C. The injector and detector temperatures were set to 200°C, the carrier gasflow rate was 0.80 mL/min, and the injection volume was 1.0 μL. The optimized conditions of analysis were then validated which included selectivity,linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ).Results: Using the validated assay and a concentration range of 0.5–20 μg/mL, the coefficient of correlation (r) for DADS was 0.9999 and the LODand LOQ for DADS were 0.3063 μg/mL and 1.0210 μg/mL, respectively. Using the validated assay and a concentration range of 0.5–20 μg/mL, thecoefficient of correlation for DATS was 0.9999 and the LOD and LOQ for DATS were 0.1986 μg/mL and 0.6621 μg/mL, respectively. The percentage ofrecovery was in the range of 98.05–101.76% and coefficient of variation ≤ 2%.Conclusion: This GC method accurately measures the levels of DADS and DATS in garlic.

scholarly journals Allicin and Digestive System Cancers: From Chemical Structure to Its Therapeutic Opportunities

2021 ◽  
Vol 11 ◽  
Author(s):  
Mahshad Sarvizadeh ◽  
Omid Hasanpour ◽  
Zari Naderi Ghale-Noie ◽  
Samaneh Mollazadeh ◽  
Mohammad Rezaei ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Digestive System ◽  
Therapeutic Potential ◽  
Diallyl Disulfide ◽  
Enzymatic Reactions ◽  
Diallyl Trisulfide ◽  
Epidemiological Evidence ◽  
Anticancer Efficacy ◽  
Skin Cancers ◽  
Gi Cancers

Digestive system cancer tumors are one of the major causes of cancer-related fatalities; the vast majority of them are colorectal or gastric malignancies. Epidemiological evidence confirmed that allium-containing food, such as garlic, reduces the risk of developing malignancies. Among all compounds in garlic, allicin has been most researched, as it contains sulfur and produces many second degradation compounds, such as sulfur dioxide, diallyl sulfide (DAS), diallyl trisulfide (DATS), and diallyl disulfide (DADS) in the presence of enzymatic reactions in gastric juice. These substances have shown anti-inflammatory, antidiabetic, antihypertensive, antifungal, antiviral, antibacterial, and anticancer efficacy, including gastrointestinal (GI) cancers, leukemia, and skin cancers. Herein, we summarize the therapeutic potential of allicin in the treatment of GI cancers.

Diallyl trisulfide and diallyl disulfide ameliorate cardiac dysfunction by suppressing apoptotic and enhancing survival pathways in experimental diabetic rats

2013 ◽  
Vol 114 (3) ◽  
pp. 402-410 ◽  
Author(s):  
Yao-Te Huang ◽  
Chun-Hsu Yao ◽  
Chia-Li Way ◽  
Kung-Wei Lee ◽  
Cheng-Yen Tsai ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Diabetic Cardiomyopathy ◽  
Biological Activities ◽  
Akt Signaling ◽  
Diabetic Rat ◽  
Diabetic Patients ◽  
Diallyl Disulfide ◽  
Therapeutic Effects ◽  
Diallyl Trisulfide ◽  
Akt Signaling Pathway

Cardiovascular disease is one of the major causes of mortality in diabetic patients. Mounting studies have shown that garlic exhibits, possibly through its antioxidant potential, diverse biological activities. In this study, we investigated the alleviating effects of garlic oil (GO) and its two major components, diallyl disulfide (DADS) and diallyl trisulfide (DATS), on diabetic cardiomyopathy in rats. Physiological cardiac parameters were obtained using echocardiography. Apoptotic cells were evaluated using TUNEL and DAPI staining. Protein expression levels were determined using Western blotting analysis. Our findings indicated that in diabetic rat hearts significantly decreased fractional shortening percentage, increased levels of nitrotyrosine, an elevated number of TUNEL-positive cells, enhanced levels of caspase 3 expression, and decreased PI3K-Akt signaling pathway activities were observed. Furthermore, all of these alterations were reversed following both GO and DATS (or DADS) administrations through increasing PI3K-Akt signaling pathway activities and inhibiting both the death receptor-dependent and the mitochondria-dependent apoptotic pathways. In conclusion, this study shows that DATS and DADS, with the efficacy order DATS > DADS, have the therapeutic potential for ameliorating diabetic cardiomyopathy. Furthermore, the therapeutic effects of GO on diabetic cardiomyopathy should be mainly from DATS and DADS.

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