scholarly journals Dual roles of brain serine hydrolase KIAA1363 in ether lipid metabolism and organophosphate detoxification

2008 ◽  
Vol 228 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Daniel K. Nomura ◽  
Kazutoshi Fujioka ◽  
Roger S. Issa ◽  
Anna M. Ward ◽  
Benjamin F. Cravatt ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Ether Lipid ◽  
Serine Hydrolase ◽  
Dual Roles ◽  
Organophosphate Detoxification

scholarly journals Triple-Knockout, Synuclein-Free Mice Display Compromised Lipid Pattern

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3078
Author(s):  
Irina A. Guschina ◽  
Natalia Ninkina ◽  
Andrei Roman ◽  
Mikhail V. Pokrovskiy ◽  
Vladimir L. Buchman
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Family Members ◽  
Ether Lipid ◽  
Brain Regions ◽  
Membrane Lipid ◽  
Lipid Binding ◽  
Synaptic Functions ◽  
Ethanolamine Plasmalogens ◽  
Lipid Pattern

Recent studies have implicated synucleins in several reactions during the biosynthesis of lipids and fatty acids in addition to their recognised role in membrane lipid binding and synaptic functions. These are among aspects of decreased synuclein functions that are still poorly acknowledged especially in regard to pathogenesis in Parkinson’s disease. Here, we aimed to add to existing knowledge of synuclein deficiency (i.e., the lack of all three family members), with respect to changes in fatty acids and lipids in plasma, liver, and two brain regions in triple synuclein-knockout (TKO) mice. We describe changes of long-chain polyunsaturated fatty acids (LCPUFA) and palmitic acid in liver and plasma, reduced triacylglycerol (TAG) accumulation in liver and non-esterified fatty acids in plasma of synuclein free mice. In midbrain, we observed counterbalanced changes in the relative concentrations of phosphatidylcholine (PC) and cerebrosides (CER). We also recorded a notable reduction in ethanolamine plasmalogens in the midbrain of synuclein free mice, which is an important finding since the abnormal ether lipid metabolism usually associated with neurological disorders. In summary, our data demonstrates that synuclein deficiency results in alterations of the PUFA synthesis, storage lipid accumulation in the liver, and the reduction of plasmalogens and CER, those polar lipids which are principal compounds of lipid rafts in many tissues. An ablation of all three synuclein family members causes more profound changes in lipid metabolism than changes previously shown to be associated with γ-synuclein deficiency alone. Possible mechanisms by which synuclein deficiency may govern the reported modifications of lipid metabolism in TKO mice are proposed and discussed.

scholarly journals Tetrahydrobiopterin and alkylglycerol monooxygenase substantially alter the murine macrophage lipidome

2015 ◽  
Vol 112 (8) ◽  
pp. 2431-2436 ◽  
Author(s):  
Katrin Watschinger ◽  
Markus A. Keller ◽  
Eileen McNeill ◽  
Mohammad T. Alam ◽  
Steven Lai ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Metabolism ◽  
Transforming Growth Factor ◽  
Ether Lipid ◽  
Mouse Bone Marrow ◽  
Monooxygenase Activity ◽  
Lipid Species ◽  
Primary Mouse ◽  
Ifn Γ

Tetrahydrobiopterin is a cofactor synthesized from GTP with well-known roles in enzymatic nitric oxide synthesis and aromatic amino acid hydroxylation. It is used to treat mild forms of phenylketonuria. Less is known about the role of tetrahydrobiopterin in lipid metabolism, although it is essential for irreversible ether lipid cleavage by alkylglycerol monooxygenase. Here we found intracellular alkylglycerol monooxygenase activity to be an important regulator of alkylglycerol metabolism in intact murine RAW264.7 macrophage-like cells. Alkylglycerol monooxygenase was expressed and active also in primary mouse bone marrow-derived monocytes and “alternatively activated” M2 macrophages obtained by interleukin 4 treatment, but almost missing in M1 macrophages obtained by IFN-γ and lipopolysaccharide treatment. The cellular lipidome of RAW264.7 was markedly changed in a parallel way by modulation of alkylglycerol monooxygenase expression and of tetrahydrobiopterin biosynthesis affecting not only various ether lipid species upstream of alkylglycerol monooxygenase but also other more complex lipids including glycosylated ceramides and cardiolipins, which have no direct connection to ether lipid pathways. Alkylglycerol monooxygenase activity manipulation modulated the IFN-γ/lipopolysaccharide–induced expression of inducible nitric oxide synthase, interleukin-1β, and interleukin 1 receptor antagonist but not transforming growth factor β1, suggesting that alkylglycerol monooxygenase activity affects IFN-γ/lipopolysaccharide signaling. Our results demonstrate a central role of tetrahydrobiopterin and alkylglycerol monooxygenase in ether lipid metabolism of murine macrophages and reveal that alteration of alkylglycerol monooxygenase activity has a profound impact on the lipidome also beyond the class of ether lipids.

scholarly journals Triple-knockout, synuclein-free mice display compromised lipid pattern

2020 ◽  
Author(s):  
Irina Guschina ◽  
Natalia Ninkina ◽  
Andrei Y. Roman ◽  
Mikhail V. Pokrovskiy ◽  
Vladimir L. Buchman
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Lipid Membranes ◽  
Ether Lipid ◽  
Fatty Acid Analysis ◽  
Membrane Lipid ◽  
Lipid Binding ◽  
Synaptic Functions ◽  
Ethanolamine Plasmalogens ◽  
Lipid Pattern

Abstract Background: Recent studies have implicated synucleins in several reactions during the biosynthesis of lipids and fatty acids in addition to their recognised role in membrane lipid binding and synaptic functions. All members of the synuclein family interact robustly with lipid membranes, and appear to be important for the physiological functions of proteins while influencing the pathological aggregation of α-synuclein. Methods: The following tissues were used for lipid and fatty acid analysis: plasma, liver and two brain areas (cortex and midbrain). Lipid classes were separated using thin-layer chromatography. Fatty acids were analysed using gas chromatography. Results: We describe the importance of long-chain polyunsaturated fatty acids (LCPUFA) and palmitic acid in liver and plasma, reduced triacylglycerol (TAG) accumulation in liver and circulated plasma non-esterified fatty acids in synuclein free mice. In midbrain, observed changes in the relative concentrations of phosphatidylcholine (PC) and cerebrosides (CER) were counterbalanced. In midbrain, we recorded a notable reduction in ethanolamine plasmalogens in synuclein free mice and consider this an important finding considering the abnormal ether lipid metabolism usually associated with neurological disorders.Conclusions: In summary, our data demonstrate that synuclein deficiency can result in alterations of PUFA synthesis, storage lipid accumulation in liver, and reduction of plasmalogens and CER, those polar lipids which are principal compounds of lipid rafts in many tissues. An ablation of all three synuclein family members resulted in more pronounced lipid modifications then previously showed by us γ-synuclein deficiency. Possible mechanisms by which synuclein deficiency may govern the reported modifications of lipid metabolism in TKO mice are proposed and discussed.

scholarly journals Increased expression of HIST1H1D in esophageal carcinoma predicts poor survival: A study base on TCGA database

2021 ◽  
Author(s):  
Shan Huang ◽  
Hanwen Huang ◽  
Mingchu Liao ◽  
Caiqun Tian ◽  
Rong Deng ◽  
...  
Keyword(s):  
Overall Survival ◽  
Lipid Metabolism ◽  
Esophageal Carcinoma ◽  
Acid Metabolism ◽  
Ether Lipid ◽  
Smooth Muscle Contraction ◽  
Poor Survival ◽  
Acid Biosynthesis ◽  
Phenylalanine Metabolism ◽  
Vascular Smooth Muscle Contraction

Abstract Background: Expression level of HIST1H1D, a linker histone H1 gene, was reported to be associated with poor prognosis in some malignant tumors. Online database showed that HIST1H1D was increased in esophageal carcinoma. The current study aimed to evaluated the role of HIST1H1D in esophageal carcinoma using online data from The Cancer Genome Atlas (TCGA).Methods. Wilcoxon signed-rank test, cox regression analysis and multivariant analysis were used to analyze the relationship between clinical characteristic and HIST1H1D expression level. Kaplan-Meier method was used to analyze the association of HIST1H1D and overall survival. Gene set enrichment analysis (GSEA) was used to identify HIST1H1D-related signaling pathway.Results. Compared to normal sample, HIST1H1D was significantly increased in esophageal carcinoma sample (p=0.000). High HIST1H1D expression was associated with poor survival (p=0.035). Univariate analysis showed that high HIST1H1D expression was associated with a poor overall survival (HR:1.19, 95% confidence interval [CI]: 1.05-1.34, p=0.01). Multivariate analysis indicated that HIST1H1D remained an independent prognostic predictor of overall survival (HR:1.18, 95% confidence interval [CI]: 1.02-1.36, p=0.03). GSEA revealed that alpha linolenic acid metabolism, arachidonic acid metabolism, histidine metabolism, vascular smooth muscle contraction, primary bile acid biosynthesis, phenylalanine metabolism and ether lipid metabolism were enriched in HIST1H1D high expression phenotype.Conclusions: HIST1H1D may sever as a potential prognostic predictor of poor survival in esophageal carcinoma. Lipid metabolism, histidine metabolism, vascular smooth muscle contraction, primary bile acid biosynthesis, phenylalanine metabolism and ether lipid metabolism may be the key signaling pathway regulated by HIST1H1D.

scholarly journals Systematic Survey of the Alteration of the Faecal Microbiota in Rats With Gastrointestinal Disorder and Modulation by Multicomponent Drugs

2021 ◽  
Vol 12 ◽  
Author(s):  
Yue Wu ◽  
Yang Wu ◽  
Hongwei Wu ◽  
Changxun Wu ◽  
Enhui Ji ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Throughput Sequencing ◽  
Intestinal Flora ◽  
Ether Lipid ◽  
Gastrointestinal Disorder ◽  
Systematic Evaluation ◽  
Faecal Microbiota ◽  
Sequencing Strategy ◽  
Comprehensive Survey ◽  
Medical Burden

Gastrointestinal disorder (GID) is a global health disease which leads to heavy public medical burden. Disorders in the intestinal flora have been found in gastrointestinal disorder patients. However, the interaction between GID and the intestinal flora in faecal has not been studied comprehensively. In addition, multicomponent drugs represented by traditional Chinese medicine (TCM) are widely used for treating GID, but their modulation of the intestinal flora has not been investigated. Therefore, in this study, a high-throughput sequencing strategy was used to investigate alterations in the intestinal flora in a rat GID model, followed by an investigation of the modulation by a representative TCM, Xiaoerfupi (XEFP) granule. The results showed that in rats with GID, the relative abundances of Erysipelotrichaceae, Lachnospiraceae, Streptococcaceae increased and that of Ruminococcaceae decreased. At the macro level, the levels of LysoPC(16:0), LysoPC(20:2), LysoPC(15:0), LysoPC(20:2 (11Z, 14Z)), LysoPC(20:1), LysoPC(15:0), LysoPC(20:0) and LysoPE (0:0/20:0) in serum increased and levels of PC(36:4), PC(38:4), PC(o-36;4), PE (MonoMe(13,5)/MonoMe(11,5)) decreased. The imbalance of metabolites was restored by XEFP through ether lipid metabolism pathway. Increase in the phyla Firmicutes/Bacteroidetes (F/B) ratio of the GID rats was restored by XEFP as well. Moreover, XEFP can relief the symptoms of GID rats by increasing bacteria Ruminococcaceae and decreasing Streptococcaceae, Erysipelotrichaceae and Lachnospiraceae in faecal microbiota level. This study represents a comprehensive survey of the interaction between GID and the intestinal flora and a systematic evaluation of modulation by a multicomponent drug.

Ether lipid metabolism

1975 ◽  
Vol 409 (3) ◽  
pp. 311-319 ◽  
Author(s):  
Harald H.O. Schmid ◽  
Patricia C. Bandi ◽  
Chang Nan-Chen ◽  
Thomas H. Madson ◽  
Wolfgang J. Baumann
Keyword(s):  
Lipid Metabolism ◽  
Ether Lipid

scholarly journals Exploration of Lipid Metabolism in Gastric Cancer: A Novel Prognostic Genes Expression Profile

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhen Xiong ◽  
Yao Lin ◽  
Yan Yu ◽  
Xianghui Zhou ◽  
Jun Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Lipid Metabolism ◽  
Expression Profile ◽  
External Validation ◽  
Ether Lipid ◽  
Gene Expression Omnibus ◽  
The Cancer Genome Atlas ◽  
Gene Profile ◽  
Significant Difference

BackgroundAlterations in lipid metabolism are increasingly being recognized. However, the application of lipid metabolism in the prognosis of gastric cancer (GC) has not yet been explored.MethodsA total of 204 lipid metabolism relative genes were analyzed in the GC cohort from The Cancer Genome Atlas (TCGA), and four independent cohorts from Gene Expression Omnibus (GEO) and one cohort from Wuhan Union Hospital were applied for external validation. Differential expression and enrichment analyses were performed between GC and normal tissue. The LASSO-Cox proportional hazard regression model was applied to select prognostic genes and to construct a gene expression profile.ResultsOur research indicated that higher expression level of AKR1B1, PLD1, and UGT8 were correlated with worse prognosis of GC patients, while AGPAT3 was correlated with better prognosis. Furthermore, we developed a gene profile composed of AGPAT3, AKR1B1, PLD1, and UGT8 suggested three groups with a significant difference in overall survival (OS). The profile was successfully validated in an independent cohort and performed well in the immunohistochemical cohort. Furthermore, we found that ether lipid metabolism, glycerophospholipid metabolism, and glycerolipid metabolism were upregulated, and fatty acid β-oxidation and other lipid peroxidation processes were reduced in GC.ConclusionCollectively, we found lipid metabolism is reliable and clinically applicable in predicting the prognosis of GC based on a novel gene profile.

Sign in / Sign up

Export Citation Format

Share Document