Immobilized fusion protein affinity chromatography combined with HPLC–ESI-Q-TOF-MS/MS for rapid screening of PPARγ ligands from natural products

Talanta ◽  
2017 ◽  
Vol 165 ◽  
pp. 508-515 ◽  
Author(s):  
Junfeng Zhu ◽  
Xiaojiao Yi ◽  
Wenhui Liu ◽  
Yingchun Xu ◽  
Shuqing Chen ◽  
...  
Keyword(s):  
Natural Products ◽  
Fusion Protein ◽  
Affinity Chromatography ◽  
Rapid Screening ◽  
Protein Affinity ◽  
Tof Ms

Daintain/AIF-1 (Allograft Inflammatory Factor-1) Promotes Erythrocyte Lysis and Heme Release Probably via Binding to Hemoglobin

2012 ◽  
Vol 67 (9-10) ◽  
pp. 525-528
Author(s):  
Yan-Ying Zhao ◽  
Wei Wang ◽  
Dong-Jing Yan ◽  
Zheng-Wang Chen
Keyword(s):  
Fusion Protein ◽  
Affinity Chromatography ◽  
Inflammatory Factor ◽  
Interacting Protein ◽  
Maldi Tof Ms ◽  
Nickel Affinity Chromatography ◽  
Erythrocyte Lysis ◽  
Free Heme ◽  
Tof Ms

Free heme is potentially cytotoxic, particularly in the presence of oxidants or activated phagocytes. Daintain/AIF-1 (allograft inflammatory factor-1) is a macrophage factor that has been implicated in the regulation of inflammation. In the present study, daintain/AIF-1 was found to induce cytolysis of erythrocytes, resulting in heme release in vitro. Furthermore, the interacting protein of daintain/AIF-1 was purified by daintain/AIF-1-6 histidine antigen fusion protein nickel affinity chromatography. MALDI-TOF-MS analysis identified hemoglobin subunit β-1 as an interacting protein of daintain/AIF-1. These data suggest that daintain/AIF-1 may be involved in heme-associated diseases

scholarly journals Escherichia coli dnaB protein. Affinity chromatography on immobilized nucleotides

1978 ◽  
Vol 253 (16) ◽  
pp. 5847-5851 ◽  
Author(s):  
E. Lanka ◽  
C. Edelbluth ◽  
M. Schlicht ◽  
H. Schuster
Keyword(s):  
Escherichia Coli ◽  
Affinity Chromatography ◽  
Protein Affinity

scholarly journals Quadrupole Time-of-Flight Mass Spectrometry: A Paradigm Shift in Toxicology Screening Applications

2019 ◽  
Vol 40 (3) ◽  
pp. 135-146 ◽  
Author(s):  
Darren Allen ◽  
Brett McWhinney
Keyword(s):  
Mass Spectrometry ◽  
Sensitivity And Specificity ◽  
Biological Samples ◽  
Time Of Flight ◽  
New Drugs ◽  
Rapid Screening ◽  
New Psychoactive Substances ◽  
Flight Mass Spectrometry ◽  
Analytical Approaches ◽  
Tof Ms

The screening of biological samples for the presence of illicit or legal substances is an important frontline tool in both clinical and forensic toxicology. In the clinical setting, drug screening is a useful tool for the clinician in improving patient care and guiding treatment. Analytical approaches for the screening of drugs in biological samples are extensive and well documented, though many rapid screening techniques often lack appropriate sensitivity and specificity, requiring careful clinical interpretation. The continuous emergence of new psychoactive substances presents a considerable analytical challenge in maintaining up-to-date methods for the detection of relevant drugs. Adapting and validating methods for the detection of new substances can be a complicated and costly undertaking. There is also a considerable lag time between the emergence of new drugs and the release of commercial assays for detection. Quadrupole time-of-flight mass spectrometry (Q-TOF-MS) has gained considerable attention over the last decade as an analytical technique that is capable of meeting the challenges of a rapidly changing drug landscape. Exhibiting both high sensitivity and specificity in drug detection, Q-TOF-MS also allows methods to be rapidly updated for newly emerging psychoactive agents. The coupling of Q-TOF-MS with techniques such as liquid or gas chromatography can provide both rapid and comprehensive screening solutions that are gaining popularity in the clinical laboratory setting.

Magnetic nanoparticles-based lactate dehydrogenase microreactor as a drug discovery tool for rapid screening inhibitors from natural products

Talanta ◽  
2020 ◽  
Vol 209 ◽  
pp. 120554 ◽  
Author(s):  
Guorong Cheng ◽  
Zifeng Pi ◽  
Zhong Zheng ◽  
Shu Liu ◽  
Zhiqiang Liu ◽  
...  
Keyword(s):  
Natural Products ◽  
Drug Discovery ◽  
Magnetic Nanoparticles ◽  
Lactate Dehydrogenase ◽  
Rapid Screening

scholarly journals Rapid Screening and Structural Characterization of Antioxidants from the Extract ofSelaginella doederleiniiHieron with DPPH-UPLC-Q-TOF/MS Method

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Gang Wang ◽  
Shun Yao ◽  
Xiu-Xiu Zhang ◽  
Hang Song
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rapid Screening ◽  
Flight Mass Spectrometry ◽  
First Time ◽  
Strong Capacity ◽  
Tof Ms

2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants fromSelaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found fromSelaginella doederleiniifor the first time and others including amentoflavone (1), robustaflavone (2), 4′-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

scholarly journals Mori Ramulus (Chin.Ph.)—the Dried Twigs of Morus alba L./Part 1: Discovery of Two Novel Coumarin Glycosides from the Anti-Hyperuricemic Ethanol Extract

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 629
Author(s):  
Jianbiao Yao ◽  
Houhong He ◽  
Jin Xue ◽  
Jianfang Wang ◽  
Huihui Jin ◽  
...  
Keyword(s):  
Natural Products ◽  
Ethanol Extract ◽  
Accurate Method ◽  
Morus Alba ◽  
Strongly Correlated ◽  
Bioactive Constituents ◽  
Antiplatelet Aggregation ◽  
Pharmacologically Active ◽  
First Time ◽  
Tof Ms

In Traditional Chinese Medicine (TCM), Mori ramulus (Chin.Ph.)—the dried twigs of Morus alba L.—is extensively used as an antirheumatic agent and also finds additional use in asthma therapy. As a pathological high xanthine oxidase (XO, EC 1.1.3.22) activity is strongly correlated to hyperuricemy and gout, standard anti-hyperuremic therapy typically involves XO inhibitors like allopurinol, which often cause adverse effects by inhibiting other enzymes involved in purine metabolism. Mori ramulus may therefore be a promissing source for the development of new antirheumatic therapeutics with less side effects. Coumarins, one of the dominant groups of bioactive constituents of M. alba, have been demonstrated to possess anti-inflammatory, antiplatelet aggregation, antitumor, and acetylcholinesterase (AChE) inhibitory activities. The combination of HPLC (DAD) and Q-TOF technique could give excellent separating and good structural characterization abilities which make it suitable to analyze complex multi-herbal extracts in TCM. The aim of this study was to develop a HPLC (DAD)/ESI-Q-TOF-MS/MS method for the identification and profiling of pharmacologically active coumarin glycosides in Mori ramulus refined extracts for used in TCM. This HPLC (DAD)/ESI-Q-TOF-MS/MS method provided a rapid and accurate method for identification of coumarin glycosides—including new natural products described here for the first time—in the crude extract of M. alba L. In the course of this project, two novel natural products moriramulosid A (umbelliferone-6-β-d-apiofuranosyl-(1→6)-β-d-glucopyranoside) and moriramulosid B (6-[[6-O-(6-deoxy-α-l-mannopyranosyl)-β-d-glucopyranosyl]oxy]-2H-1-benzopyran-1-one) were newly discovered and the known natural product Scopolin was identified in M. alba L. for the first time.

scholarly journals Simple and Rapid Screening for Psychotropic Natural Products Using Direct Analysis in Real Time (DART)-TOFMS

2009 ◽  
Vol 129 (6) ◽  
pp. 719-725 ◽  
Author(s):  
Maiko KAWAMURA ◽  
Ruri KIKURA-HANAJIRI ◽  
Yukihiro GODA
Keyword(s):  
Natural Products ◽  
Real Time ◽  
Direct Analysis ◽  
Rapid Screening
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