Electrochemical sensor for tricyclic antidepressants with low nanomolar detection limit: Quantitative Determination of Amitriptyline and Nortriptyline in blood

Talanta ◽  
2021 ◽  
pp. 123072
Author(s):  
Marcin Guzinski ◽  
Ernő Lindner ◽  
Bradford Pendley ◽  
Edward Chaum
Keyword(s):  
Detection Limit ◽  
Quantitative Determination ◽  
Electrochemical Sensor ◽  
Tricyclic Antidepressants ◽  
Nanomolar Detection

Liquid Chromatographic Determination of Sulfamoyldapsone in Swine Tissues

1987 ◽  
Vol 70 (6) ◽  
pp. 1031-1032
Author(s):  
Yuuko S Endoh ◽  
Ryozo Yamaoka ◽  
Nobuo Sasaki
Keyword(s):  
Detection Limit ◽  
Quantitative Determination ◽  
Chromatographic Determination ◽  
Alumina Column ◽  
Average Recovery ◽  
Diaminodiphenyl Sulfone ◽  
Alumina Column Chromatography ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for the quantitative determination of sulfamoyldapsone (2-sulfamoyl-4,4'-diaminodiphenyl sulfone) in swine muscle, liver, kidney, and fat. Sulfamoyldapsone was extracted from tissues with acetonitrile saturated with n-hexane. The extract was washed with n-hexane saturated with acetonitrile, concentrated, and cleaned up by alumina column chromatography. Sulfamoyldapsone was separated on an ODS column by using acetonitrile-methanol-water (6 + 18 + 76) and was detected at 292 nm. Overall average recovery of sulfamoyldapsone added to tissues at levels of 0.1 and 0.5 /μg/g was 93.3% ± 6.0. Detection limit was 0.02 μg/g in these tissues.

scholarly journals Spectrometric Determination of Heavy Metals Present in Mango Fruit of Nepali Origin

2020 ◽  
Vol 1 (1) ◽  
pp. 72-77
Author(s):  
Naresh Pant ◽  
Anup Subedee ◽  
Ram Bahadur Gharti ◽  
Santu Shrestha
Keyword(s):  
Heavy Metals ◽  
Detection Limit ◽  
Quantitative Determination ◽  
Standard Method ◽  
Random Sampling ◽  
Sampling Method ◽  
Spectrometric Method ◽  
Mango Fruit ◽  
Atomic Absorption Spectrometric

Quantitative determination of heavy metals; Fe, Zn, Co, Pb and Ni in Mango fruit of Nepali origin, locally sourced was carried out. Fifteen Mango samples were collected by random sampling method, converted into analyte sample by standard method and analyzed by using Atomic absorption spectrometric method. The amount of heavy metals Fe, Zn, Co and Ni present in sample was, 0.570±0.48, 0.510±0.031, 0.431±0.021, 0.106±0.003 mg/kg respectively. The results indicated the concentration of Zn & Co were higher (WHO 0.320 & 0.05), and the concentration of Nickel (Ni) was below the maximum permissible limit issued by WHO. The concentration of the Lead (Pb) was found below the detection limit of the instrument used.

Quantitative Endotoxin Determination In Blood With A Chromogenic Substrate

1981 ◽  
Author(s):  
L L M Thomas ◽  
A Sturk ◽  
M C Schaap ◽  
H ten Cate ◽  
J W ten Cate
Keyword(s):  
Detection Limit ◽  
Quantitative Determination ◽  
Detection Threshold ◽  
Blood Cultures ◽  
Chromogenic Substrate

A method, its optimalization and a solution to specific problems is described for the quantitative determination of endotoxins in blood. The method is based upon the endotoxin-dependent activation of a proenzyme, present in limulus ame- bocyte lysate. This activated enzyme is measured with the chromogenic substrate S2222. Possible inhibitors and activated coagulationfactors interfering in the assay are removed by dilution and boiling. The method is shown to be fast (2½ hr), sensitive and reproducible with a detection limit of 10 pg/ml.The endotoxin assay was found to have a good correlation with results of blood cultures.The detection threshold of the endotoxin assay compared with the number of circulating gramnegative organisms and CRD-measurements will also be shown.

scholarly journals Direct Assay for Cobalamin Bound to Transcobalamin (Holo-Transcobalamin) in Serum

2002 ◽  
Vol 48 (3) ◽  
pp. 526-532 ◽  
Author(s):  
Marius Ulleland ◽  
Ingar Eilertsen ◽  
Edward V Quadros ◽  
Sheldon P Rothenberg ◽  
Sergey N Fedosov ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Detection Limit ◽  
Quantitative Determination ◽  
Reliable Method ◽  
Solid Phase ◽  
Reference Interval ◽  
Magnetic Microspheres ◽  
Affinity Constant ◽  
Capture Assay

Abstract Background: Only cobalamin carried by transcobalamin (holo-transcobalamin) is available for cellular uptake and hence is physiologically relevant. However, no reliable or accurate methods for quantifying holo-transcobalamin are available. We report a novel holo-transcobalamin assay based on solid-phase capture of transcobalamin. Methods: A monoclonal antibody specific for human transcobalamin with an affinity constant >1010 L/mol was immobilized on magnetic microspheres to capture and concentrate transcobalamin. The cobalamin bound to transcobalamin was then released and assayed by a competitive binding radioassay. The quantification of holo-transcobalamin was accomplished using calibrators composed of recombinant, human holo-transcobalamin. Results: The assay was specific for holo-transcobalamin and had a detection limit of 5 pmol/L. Within-run and total imprecision (CV) was 5% and 8–9%, respectively. The working range (CV <20%) was 5–370 pmol/L. Dilutions of serum were linear in the assay range. The recovery of recombinant, human holo-transcobalamin added to serum was 93–108%. A 95% reference interval of 24–157 pmol/L was established for holo-transcobalamin in 105 healthy volunteers 20–80 years of age. For 72 of these sera, holo-haptocorrin and total cobalamin were also determined. Whereas holo-haptocorrin correlated well (r2 = 0.87) with total cobalamin, holo-transcobalamin correlated poorly (r2 = 0.23) with total cobalamin or holo-haptocorrin. Conclusions: The solid-phase capture assay provides a simple, reliable method for quantitative determination of holo-transcobalamin in serum.

Development of an Analytical Protocol for the Determination of Polybrominated Biphenyls in Water by Enzyme-Linked Immunosorbent Assay

2011 ◽  
Vol 347-353 ◽  
pp. 1537-1541 ◽  
Author(s):  
Han Yu Chen ◽  
Hui Sheng Zhuang
Keyword(s):  
Detection Limit ◽  
Quantitative Determination ◽  
Incubation Time ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Concentration ◽  
Polybrominated Biphenyls ◽  
Analytical Protocol ◽  
Optimized Conditions

A novel immunoassay has been developed for the quantitative determination of polybrominated biphenyls (PBBs) using indirect competitive format. A new method was developed to synthesize PBBs congener (PCB15) hapten and its artificial immunogen, then the polyclonal antibodies. The assay was optimized concerning the coating conjugate and antibody concentration, incubation time and temperature, the tolerance to organic solvents and so on. Under optimized conditions, PBB15 can be determined in the concentration range of 0.01-100 μg L-1 with a detection limit of 0.02 μg L-1. The cross-reactivities of the assays were below 8%. While water samples could be analyzed directly.

Molecularly imprinted electrochemical sensor for selective determination of oxidized glutathione

2015 ◽  
Vol 7 (5) ◽  
pp. 2210-2214 ◽  
Author(s):  
Hong Hai ◽  
Xiaodong An ◽  
Jianping Li
Keyword(s):  
Detection Limit ◽  
Electrochemical Sensor ◽  
The Other ◽  
Oxidized Glutathione ◽  
Molecularly Imprinted ◽  
Selective Determination

A molecularly imprinted sensor was prepared for the selective determination of oxidized glutathione with a detection limit of 1.8 × 10−9 mol L−1, which was lower than most of the other methods.

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