Glycoprotein isolated from Solanum nigrum L. inhibits the DNA-binding activities of NF-κB and AP-1, and increases the production of nitric oxide in TPA-stimulated MCF-7 cells

Aim. Studying of black nightshade young plants’ response to the induced mechanical stresses. Methods. Researches conducted in small plot fi eld experiments. Results. Change of sensitivity level of Solanum nigrum L. plants depending on phases of their development at the moment of damage of elevated parts has been proven. Owing to loss of the surface capable to photosynthesis, there is an essential decrease in volumes of photosynthesis at plants of weed survived and their possibilities of ontogeny passage. The deep induced dis- stresses reduce biological effi ciency of plants, their ability to accumulate weight and to form seeds and even lead them to death. Conclusions. The defi ned principles of response of weed plants to the induced mechanical dis-stresses are can be used for working out and ecological receptions of crops protection from weeds.

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Phytochemical composition of leaves and stems of Solanum nigrum L. and Solanum dulcamara L. (Solanaceae) from Bulgaria

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1031/1/012091 ◽

2021 ◽

Vol 1031 (1) ◽

pp. 012091

Author(s):

V T Popova ◽

M A Stoyanova ◽

T A Ivanova ◽

A S Stoyanova ◽

I Z Dimitrova-Dyulgerova

Keyword(s):

Solanum Nigrum ◽

Solanum Dulcamara ◽

Phytochemical Composition ◽

Solanum Nigrum L

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Cytokine and inflammatory mediators are associated with cytotoxic, anti-inflammatory and apoptotic activity of honeybee venom

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0182 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Mohamed A. Salama ◽

Mohamed A. Younis ◽

Roba M. Talaat

Keyword(s):

Nitric Oxide ◽

Cell Lines ◽

Cancer Cell ◽

Hela Cells ◽

No Production ◽

Hep G2 ◽

Normal Cells ◽

Tnf Α ◽

Ifn Γ ◽

Mcf 7

AbstractObjectiveThe present study aimed to evaluate cytotoxic, apoptotic, and anti-inflammatory properties of bee venom (BV) as well as changes in cytokine secretion levels and nitric oxide (NO) production using three different cancer cell lines [liver (Hep-G2), breast (MCF-7), and cervical (HPV-18 infected HeLa cells)] and two normal cells (splenocytes and macrophages (MQ).MethodsCytotoxic activity of BV against tumor cell lines and normal splenocytes/MQ was tested by MTT assay. By ELISA (ELISA); Tumor necrosis factor (TNF-α), Interleukine (IL-10) and interferon (IFN-γ) were measured. Caspase three expressions was evaluated using reverse transcription-polymerase chain reaction (RT-PCR). Nitric oxide (NO) was estimated using a colorimetric assay.ResultsBV has a significant cytotoxic effect on all cell lines in a dose- and time-dependent manner; none of them was toxic for normal cells. Treating Hep-G2 cells with BV showed a reduction in IL-10, elevation in TNF-α with no change in IFN-γ level. MCF-7 cells have low IL-10 and TNF-α and high IFN-γ production level. Elevation of IL-10 and IFN-γ coincides with a reduction in TNF-α level was demonstrated in HeLa cells. The expression of Caspase three was dramatically increased with elevation in BV concentration in all tested cancer cell lines. A gradual decrease in NO production by MQ with increasing BV dose was observed.ConclusionTaken together, our results stressed on the importance of BV as a potent anti-tumor agent against various types of cancers (Liver, Breast, and Cervix). Further steps towards the use of BV for pharmacological purposes must be done.

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