Prenatal exposure to di- n -butyl phthalate disrupts the development of adult Leydig cells in male rats during puberty

Toxicology ◽  
2017 ◽  
Vol 386 ◽  
pp. 19-27 ◽  
Author(s):  
Xiaomin Chen ◽  
Linxi Li ◽  
Huitao Li ◽  
Hongguo Guan ◽  
Yaoyao Dong ◽  
...  
Keyword(s):  
Prenatal Exposure ◽  
Leydig Cells ◽  
Male Rats ◽  
Adult Leydig Cells ◽  
Butyl Phthalate

scholarly journals Effects of Osteocalcin on Synthesis of Testosterone and INSL3 during Adult Leydig Cell Differentiation

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Gulfidan Coskun ◽  
Leman Sencar ◽  
Abdullah Tuli ◽  
Dilek Saker ◽  
Mustafa Muhlis Alparslan ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Postnatal Development ◽  
Leydig Cell ◽  
Leydig Cells ◽  
Development Process ◽  
Gnrh Antagonist ◽  
Serum Testosterone ◽  
Male Rats ◽  
Significant Difference ◽  
Adult Leydig Cells

Proliferation and differentiation of adult Leydig cells are mainly completed in puberty. In many studies, apart from normal postnatal development process, it is widely indicated that, through administrating EDS, Leydig cell population is eliminated and regenerated. It is believed that osteocalcin released from osteoblasts, which is responsible for modulating bone metabolism, induces testosterone production in Leydig cells, independent of the HPG axis. In addition, INSL3 produced by Leydig cells, such as testosterone, plays a critical role in bone metabolism and is known to reflect the development process and functional capacities of Leydig cells. This study is aimed at investigating OC-mediated testosterone regulation and INSL3 synthesis during differentiation of adult Leydig cells that are independent of LH. For this purpose, male rats were divided into 2 groups: prepubertal normal rats and adult EDS-injected rats. Each group was divided into 4 subgroups in which GnRH antagonist or OC was applied. After adult Leydig cells completed their development, testicular tissue samples obtained from the sacrificed rats were examined by light-electron microscopic, immunohistochemical, and biochemical methods. Slight upregulation in 3βHSD, INSL3, and GPRC6A expressions along with the increase in serum testosterone levels was observed in groups treated with osteocalcin against GnRH antagonist. In addition, biochemical and microscopic findings in osteocalcin treated groups were similar to those in control groups. While there was no significant difference in the number of Leydig cells reported, the presence of a significant upregulation in INSL3 and GPRC6A expressions and the increase in serum testosterone and ucOC levels were observed. After evaluation of findings altogether, it is put forward that, for the first time in this study, although osteocalcin treatment made no significant difference in the number of Leydig cells, it increased the level of testosterone through improving the function of existing adult Leydig cells during normal postnatal development process and post-EDS regeneration. This positive correlation between osteocalcin-testosterone and osteocalcin-INSL3 is concluded to be independent of LH at in vivo conditions.

scholarly journals Prenatal exposure to di-n-butyl phthalate induces erectile dysfunction in male adult rats

2021 ◽  
Vol 219 ◽  
pp. 112323
Author(s):  
Xiang Zhou ◽  
Tongtong Zhang ◽  
Lebin Song ◽  
Yichun Wang ◽  
Qijie Zhang ◽  
...  
Keyword(s):  
Erectile Dysfunction ◽  
Prenatal Exposure ◽  
Adult Rats ◽  
Male Adult ◽  
Butyl Phthalate

scholarly journals The Leydig Cell MEK/ERK Pathway Is Critical for Maintaining a Functional Population of Adult Leydig Cells and for Fertility

2011 ◽  
Vol 25 (7) ◽  
pp. 1211-1222 ◽  
Author(s):  
Soichi Yamashita ◽  
Ping Tai ◽  
Jean Charron ◽  
CheMyong Ko ◽  
Mario Ascoli
Keyword(s):  
Leydig Cell ◽  
Leydig Cells ◽  
Erk Pathway ◽  
Adult Leydig Cells

scholarly journals Development of fetal and adult Leydig cells

2019 ◽  
Vol 18 (4) ◽  
pp. 323-330 ◽  
Author(s):  
Yuichi Shima
Keyword(s):  
Leydig Cells ◽  
Adult Leydig Cells

scholarly journals High Doses of Caffeine during the Peripubertal Period in the Rat Impair the Growth and Function of the Testis

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Minji Park ◽  
Yuri Choi ◽  
Hyeonhae Choi ◽  
Ju-Yearn Yim ◽  
Jaesook Roh
Keyword(s):  
Leydig Cells ◽  
Ex Vivo ◽  
Body Weight Gain ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
High Doses ◽  
And Function ◽  
The Impact ◽  
Caffeine Exposure

Prenatal caffeine exposure adversely affects the development of the reproductive organs of male rat offspring. Thus, it is conceivable that peripubertal caffeine exposure would also influence physiologic gonadal changes and function during this critical period for sexual maturation. This study investigated the impact of high doses of caffeine on the testes of prepubertal male rats. A total of 45 immature male rats were divided randomly into three groups: a control group and 2 groups fed 120 and 180 mg/kg/day of caffeine, respectively, via the stomach for 4 weeks. Caffeine caused a significant decrease in body weight gain, accompanied by proportional decreases in lean body mass and body fat. The caffeine-fed animals had smaller and lighter testes than those of the control that were accompanied by negative influences on the histologic parameters of the testes. In addition, stimulated-testosterone ex vivo production was reduced in Leydig cells retrieved from the caffeine-fed animals. Our results demonstrate that peripubertal caffeine consumption can interfere with the maturation and function of the testis, possibly by interrupting endogenous testosterone secretion and reducing the sensitivity of Leydig cells to gonadotrophic stimulation. In addition, we confirmed that pubertal administration of caffeine reduced testis growth and altered testis histomorphology.

Genetic research and structural dysplasia assessment of anorectal malformations in neonatal male rats induced by di(n-butyl) phthalate

2014 ◽  
Vol 31 (3) ◽  
pp. 261-268 ◽  
Author(s):  
Zhi-Hong Liu ◽  
En-Hui Li ◽  
Dong-Liang Xu ◽  
Wen-Lan Sun ◽  
Yan Hong ◽  
...  
Keyword(s):  
Genetic Research ◽  
Anorectal Malformations ◽  
Male Rats ◽  
Butyl Phthalate

Protective effects of Lactobacillus plantarum strain P1 against toxicity of the environmental oestrogen di-n-butyl phthalate in rats

2020 ◽  
Vol 11 (8) ◽  
pp. 803-813
Author(s):  
X. Shi ◽  
C. Hu ◽  
S. Cai ◽  
X. Tao ◽  
Y. Zhou ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Therapeutic Strategy ◽  
Male Rats ◽  
Protective Effects ◽  
Oral Gavage ◽  
Butyl Phthalate ◽  
Maize Oil

Phthalates are contaminants widely distributed in the food-chain, and they are considered as important environmental oestrogens in our lives. In the present study, eight strains of lactic acid bacteria were isolated for their ability to adsorb di-n-butyl-phthalate (DBP), and one of the strains, Lactobacillus plantarum strain P1, was selected for more detailed analyses of its phthalate adsorption capacity in vitro. This study also evaluated the in vivo protective effects of strain P1 against DBP toxicity in rats. Sixteen rats were divided into four groups, and animals received by oral gavage every other day for a period of one month saline with or without strain P1 at 2×1011 cfu/kg followed by maize oil with or without DBP (50 mg/kg). Strain P1 could adsorb more DBP than saline alone, and the concentration of mono-n-butyl phthalate in urine was decreased in animals receiving P1. Furthermore, oestrogenic effects of the different treatments were assessed through counting of sperm and observation of testis, and strain P1 could protect the sexual organs of male rats. Our results suggested that P1 is effective against phthalate toxicity due to its ability to adsorb DBP in vivo and could be considered as a new dietary therapeutic strategy against environmental phtalate toxicity.

Risperidone induced reproductive toxicity in male rats targeting leydig cells and hypothalamic–pituitary–gonadal axis by inducing oxidative stress

Andrologia ◽  
2020 ◽  
Author(s):  
Gözde Görmüş ◽  
Sinem Ilgın ◽  
Merve Baysal ◽  
Abdullah Burak Karaduman ◽  
Volkan Kılıç ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Leydig Cells ◽  
Reproductive Toxicity ◽  
Male Rats
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