A comparison of acute and long-term effects of industrial multiwalled carbon nanotubes on human lung and immune cells in vitro

We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH2)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH2s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH2s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH2s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH2s/ORC composites with increasing the concentration of MWCNT-NH2s in composite. The in-vitro procoagulation test shows that the MWCNT-NH2s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH2s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH2s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH2s/ORC gauze is discussed.

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Enhanced laser thermal ablation for the in vitro treatment of liver cancer by specific delivery of multiwalled carbon nanotubes functionalized with human serum albumin

International Journal of Nanomedicine ◽

10.2147/ijn.s15841 ◽

2011 ◽

pp. 129 ◽

Cited By ~ 47

Author(s):

Cornel Iancu ◽

Constantin ◽

Catoi Cornel ◽

Flaviu ◽

Rares ◽

...

Keyword(s):

Carbon Nanotubes ◽

Human Serum Albumin ◽

Liver Cancer ◽

Serum Albumin ◽

Human Serum ◽

Multiwalled Carbon Nanotubes ◽

Thermal Ablation ◽

Multiwalled Carbon ◽

In Vitro Treatment

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Different Cellular Response of Human Mesothelial Cell MeT-5A to Short-Term and Long-Term Multiwalled Carbon Nanotubes Exposure

BioMed Research International ◽

10.1155/2017/2747215 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Li Ju ◽

Wei Wu ◽

Min Yu ◽

Jianlin Lou ◽

Hao Wu ◽

...

Keyword(s):

Carbon Nanotubes ◽

Cell Motility ◽

Multiwalled Carbon Nanotubes ◽

Mesothelial Cell ◽

Continuous Exposure ◽

Short Term ◽

Multiwalled Carbon ◽

Long Term Treatment ◽

Human Mesothelial Cell

Despite being a commercially important product, multiwalled carbon nanotubes (MWCNTs) continue to raise concerns over human health due to their structural similarity to asbestos. Indeed, exposure to MWCNT has been shown to induce lung cancer and even mesothelioma, but contradictory results also exist. To clarify the potentially carcinogenic effects of rigid and rod-like MWCNT and to elucidate the underlying mechanisms, the effects of MWCNT on human mesothelial cell MeT-5A were examined throughout 3 months of continuous exposure, including cytotoxicity, genotoxicity, and cell motility. It was found that MWCNT did not affect MeT-5A cell proliferation at 10 μg/cm2 within 72 h treatment, but under the same condition, MWCNT induced genotoxicity and perturbed cell motility. In addition, MeT-5A cells demonstrated different cellular responses to MWCNT after short-term and long-term exposure. Taken together, our results indicated a possible carcinogenic potential for MWCNT after long-term treatment, in which Annexin family proteins might be involved.

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Nitrogen-Doped Multiwalled Carbon Nanotubes Inhibit Fat Deposition via Immunomodulatory Actions

10.21203/rs.3.rs-960318/v1 ◽

2021 ◽

Author(s):

Haifang Li ◽

Xue Xiao ◽

Geng Hu ◽

Dalin He ◽

Wenqian Zhang ◽

...

Keyword(s):

Carbon Nanotubes ◽

Multiwalled Carbon Nanotubes ◽

Mammalian Cells ◽

Fat Metabolism ◽

Fat Deposition ◽

The Body ◽

Inflammatory Factors ◽

Multiwalled Carbon ◽

Nitrogen Doped

Abstract Multiwalled carbon nanotubes (MWCNTs) offer immense opportunities to deliver drug and biomolecules to targeted tissues. However, it’s unclear for us about their effects on fat metabolism. Here, we demonstrate that nitrogen-doped carboxylate-functionalized MWCNTs (N-MWCNTs) inhibit fat deposition both in vivo and in vitro primarily by suppressing adipogenesis. N-MWCNTs show good biocompatability in HEK293 mammalian cells. Intramuscular administration of N-MWCNTs does not affect the body weight gain and feed intake of mice, but reduces the fat mass. In in vitro-cultured adipocytes, N-MWCNTs suppress fat accumulation, accompanying with decreased and increased expression of adipogenic and lipolysis genes, respectively. Transcriptome analysis further certified the N-MWCNT alteration of fat metabolism-related genes. Interestingly, we observed the phagocytosis of N-MWCNTs by macrophage-like cells via TEM imaging. The mRNA sequencing data also showed remarkable variation of the genes involved in TLRs pathway, ultimately leading to down- or up-regulation of inflammatory factors, of which Tnfα, Il1, Il7, Il10, and Il12 are decreased, whereas Il6 and Il11 are increased. In conclusion, N-MWCNTs induce the production of inflammatory cytokines through immune responses, which trigger the reduction of fat deposition. These findings support the usage of N-MWCNTs as a promising delivery for anti-obesity agents.

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Profibrotic Activity of Multiwalled Carbon Nanotubes Upon Prolonged Exposures in Different Human Lung Cell Types

Applied In Vitro Toxicology ◽

10.1089/aivt.2017.0033 ◽

2019 ◽

Vol 5 (1) ◽

pp. 47-61 ◽

Cited By ~ 11

Author(s):

Savvina Chortarea ◽

Fikad Zerimariam ◽

Hana Barosova ◽

Dedy Septiadi ◽

Martin J.D. Clift ◽

...

Keyword(s):

Carbon Nanotubes ◽

Multiwalled Carbon Nanotubes ◽

Human Lung ◽

Cell Types ◽

Multiwalled Carbon

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Integration of Multiwalled Carbon Nanotubes in Bulk Heterojunction CdSe/PCPDTBT Hybrid Solar Cells

Materials Science Forum ◽

10.4028/www.scientific.net/msf.929.150 ◽

2018 ◽

Vol 929 ◽

pp. 150-157

Author(s):

Alfian Ferdiansyah Madsuha ◽

Nofrijon Sofyan ◽

Akhmad Herman Yuwono ◽

Michael Krueger

Keyword(s):

Carbon Nanotubes ◽

Solar Cells ◽

Multiwalled Carbon Nanotubes ◽

Bulk Heterojunction ◽

Photovoltaic Performance ◽

Hybrid Solar Cells ◽

Cdse Qds ◽

Multiwalled Carbon ◽

Simple Method

In this work, the development of solution-processed bulk heterojunction hybrid solar cells based on CdSe quantum dot (QD) and conjugated polymer poly [2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta [2,1-b;3,4-b] dithiophene)-alt-4,7-(2,1,3-benzothiadiazole)], PCPDTBT was performed. The photoactive layer was formed by integrating CdSe QDs onto multiwalled carbon nanotubes (CNTs). A simple method of thiol functionalization in the interface CNTs and CdSe QDs has been investigated. Integration of CNTs enhances long-term performance of solar cells devices. Initial PCE values of about 1.9 % under AM1.5G illumination have been achieved for this hybrid CNT-CdSe photovoltaic device. In addition, the long-term stability of the photovoltaic performance of the devices was investigated and found superior to CdSe QD only based devices. About 84 % of the initial PCE remained after storage in a glove box for one year without any further encapsulation. It is concluded that the improvement is mainly due to a strong binding between thiol functionalized CNTs and CdSe QDs, resulting preservation of the nanomorphology of the hybrid film over time.

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