Cadmium as oxidative stress inducer in brain of subacutely exposed rats and its implication on zinc level

We investigated oxidative stress markers and metal ions in patients with Alzheimer’s disease (AD). The serum levels of ceruloplasmin (CER), C-reactive protein (CRP), uric acid (UA), homocysteine (Hcy), copper, iron, and zinc were determined in 125 patients with AD (mild, n = 2 8; moderate, n = 42; and severe, n = 55) and 40 healthy control (HC) participants. Compared to HC, CER and UA levels were significantly lower in moderate and severe AD groups, whereas CRP and Hcy levels were significantly higher in the severe AD group. Copper level was significantly higher in moderate and severe AD groups than the other groups. Compared to HC, iron level was significantly higher in patients with AD, whereas zinc level was significantly lower in patients with AD. In patients with AD, the severity of cognitive impairment was positively correlated with CER, UA, and zinc levels, whereas it was negatively correlated with copper level. Taken together, our findings provide a novel approach to assess AD progression.

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Paraquat: An Oxidative Stress Inducer

Herbicides - Properties, Synthesis and Control of Weeds ◽

10.5772/32590 ◽

2012 ◽

Cited By ~ 11

Author(s):

Ramiro Lascano ◽

Nacira Munoz ◽

German Robert ◽

Marianela Rodriguez ◽

Mariana Melchiorre ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Stress Inducer

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Correlation between Serum Zinc Level and Erythrocyte Superoxide Dismutase (SOD) Activity in Non-frail and Frail Geriatric Patients

World Nutrition Journal ◽

10.25220/wnj.v01i1.0008 ◽

2017 ◽

Vol 1 (1) ◽

pp. 34

Author(s):

Rosnah Pinontoan ◽

Savitri Sayogo ◽

Arya Govinda Roosheroe

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Venous Blood ◽

Serum Zinc ◽

Zinc Level ◽

Zn Deficiency ◽

Serum Zinc Level ◽

Physical Measurements ◽

Significant Difference ◽

The Mean

Introduction: Frailty syndrome (FS), as a health problem in elderly, can interfere with the quality of life. Many factors play roles in its occurrence, one of which is oxidative stress. Superoxide dismutase (SOD), in which one of its components is zinc (Zn), is one of the endogenous antioxidants that plays a role in preventing oxidative stress. This study was aimed to know the correlation between serum zinc level (sZn) and erythrocyte SOD (eSOD) activity in non-frail and frail geriatric patients.Methods: This was cross-sectional study with consecutive sampling method conducted in the Geriatric Clinic Cipto Mangunkusumo hospital, from August through September 2014, on 60 patients (30 patients for each group of non-frail and frail). Data were collected from interviews, physical measurements, and venous blood sampling.Results: Among 90% of the subjects had low Zn intake, at the same time, the mean of sZn was 8.41 ± 1.36mol/l and 93.3% of the subjects had experienced Zn deficiency. The mean of eSOD activity was 1526 ± 508U/gHb, and there was no significant difference between the groups. There were no significant correlation between sZn and eSOD activity both in the overall subject (r = -0.04; p = 0.076), as well as the non-frail group (r = -0.099; p=0.604) and frail group (r = 0.01; p = 0.957).Conclusion: Serum zinc level did not have any significant correlation with the eSOD activity, neither on overall or both non-frail and frail groups.

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Abstract C11: The oxidative stress inducer elesclomol requires copper chelation for its anticancer activity

10.1158/1535-7163.targ-09-c11 ◽

2009 ◽

Cited By ~ 3

Author(s):

Masazumi Nagai ◽

Nha Vho ◽

Elena Kostik ◽

Suqin He ◽

Jane Kepros ◽

...

Keyword(s):

Oxidative Stress ◽

Anticancer Activity ◽

Copper Chelation ◽

Oxidative Stress Inducer

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Zinc Transporter ZIP12 Maintains Zinc Homeostasis and Protects Spermatogonia From Oxidative Stress During Spermatogenesis

10.21203/rs.3.rs-800872/v1 ◽

2021 ◽

Author(s):

Xinye Zhu ◽

Chengxuan Yu ◽

Wangshu Wu ◽

Lei Shi ◽

Chenyi Jiang ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Viability ◽

Male Fertility ◽

Sperm Quality ◽

Zinc Level ◽

Zinc Transporter ◽

Zinc Homeostasis ◽

Mouse Testis ◽

Zinc Transporters ◽

Wide Range

Abstract Background: Overwhelming evidences now suggest oxidative stress is a major cause of sperm dysfunction and male infertility. Zinc is an important non-enzyme antioxidant with a wide range of biological functions and plays a significant role in preserving male fertility. Notably, zinc trafficking through the cellular and intracellular membrane is endorsed by precise families of zinc transporters, i.e. SLC39s/ZIPs and SLC30s/ZnTs. However, the expression and function of zinc transporters in the male germ cells were rarely reported. The aim of this study is to determine the crucial zinc transporter responsible for the maintenance of spermatogenesis.Methods: In the present study, we investigated the expression of all fourteen ZIP members in mouse testis and further analyzed the characteristic of ZIP12 expression in testis and spermatozoa by qRT-PCR, immunoblot and immunohistochemistry analyses. To explore the antioxidant role of ZIP12 in spermatogenesis, an obese mouse model fed with high-fat-diet was employed to confirm the correlation between ZIP12 expression level and sperm quality. Furthermore, ZIP12 expression in response to oxidative stress in a spermatogonia cell line, C18-4 cells, was determined and its function involved in regulating cell viability and apoptosis was investigated by RNAi experiment. Results: We initially found that ZIP12 expression in mouse testis was significantly high compared to other members of ZIPs and its mRNA and protein were intensively expressed in testis rather than the other tissues. Importantly, ZIP12 was intensively abundant in spermatogonia and spermatozoa, both in mice and humans. Moreover, ZIP12 expression in testis significantly decreased in obese mice, which associated with reduced sperm zinc content, excessive sperm ROS, poor sperm quality and male subfertility. Similarly, its expression in C18-4 cells significantly declined in response to oxidative stress. Additionally, reduced ZIP12 expression by RNAi associated with a decline in zinc level subsequently caused low cell viability and high cell apoptosis in C18-4 cells. Conclusions: The zinc transporter ZIP12 is intensively expressed in testis, especially in spermatogonia and spermatozoa. ZIP12 may play a key role in maintaining intracellular zinc level in spermatogonia and spermatozoa, by which it resists oxidative stress during spermatogenesis and therefore preserves male fertility.

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Synthesis of Novel Selenocyanates and Evaluation of Their Effect in Cultured Mouse Neurons Submitted to Oxidative Stress

10.26434/chemrxiv.12251171 ◽

2020 ◽

Author(s):

Tiago Elias Allievi Frizon ◽

José H. Cararo ◽

Sumbal Saba ◽

Gustavo C. Dal-Pont ◽

Monique Michels ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Thiobarbituric Acid ◽

Positive Control ◽

Protein Carbonyl ◽

Oxidative Challenge ◽

Hydroxy Esters ◽

Old Mice ◽

Oxidative Stress Inducer

Herein we report the synthesis of novel selenocyanates and assessment of their effect on the oxidative challenge elicited by hydrogen peroxide (H2O2) in cultured mouse neurons. First, α-methylene-β-hydroxy esters were prepared as precursors of allylic bromides. A reaction involving the generated bromides and sodium selenocyanate was conducted to produce the desired selenocyanates (3a-f). We next prepared cultures of neurons from 7-day-old-mice (n = 36). H2O2 (10⁻5 M) was added into the culture flasks as an oxidative stress inducer, alone or combined with one of each designed compounds. PhSe)2 was used as positive control. It was carried out assessment of lipid (thiobarbituric acid reactive species, 4-hydroxy-2’-nonenal, 8-isoprostane), DNA (8-hydroxy-2’-deoxyguanosine) and protein (carbonyl) modification parameters. Finally, catalase and superoxide dismutase activities were also evaluated. Among the compounds, 3b, 3d and 3f exhibited the most pronounced pattern of antioxidant activity, similar to (PhSe)2. These novel aromatic selenocyanates could be promising to be tried in most sophisticated in vitro studies or even at preclinical level.

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