Multiple functionalities of functional nucleic acids for developing high-performance lateral flow assays

2022 ◽  
pp. 116529
Author(s):  
Jia Wang ◽  
Longjiao Zhu ◽  
Tianshun Li ◽  
Xiangyang Li ◽  
Kunlun Huang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
High Performance ◽  
Lateral Flow ◽  
Lateral Flow Assays ◽  
Functional Nucleic Acids

scholarly journals Integrating high-performing electrochemical transducers in lateral flow assay

2021 ◽  
Author(s):  
Antonia Perju ◽  
Nongnoot Wongkaew
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Analytical Performance ◽  
Label Free ◽  
High Performing ◽  
Lateral Flow Assays ◽  
Electrochemical Transducers

AbstractLateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

scholarly journals A Low-Cost, High-Performance System for Fluorescence Lateral Flow Assays

Biosensors ◽  
2013 ◽  
Vol 3 (4) ◽  
pp. 360-373 ◽  
Author(s):  
Linda Lee ◽  
Eric Nordman ◽  
Martin Johnson ◽  
Mark Oldham
Keyword(s):  
High Performance ◽  
Low Cost ◽  
Lateral Flow ◽  
Lateral Flow Assays ◽  
Performance System

scholarly journals Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1058
Author(s):  
Zidane Qriouet ◽  
Yahia Cherrah ◽  
Hassan Sefrioui ◽  
Zineb Qmichou
Keyword(s):  
High Performance ◽  
Drugs Of Abuse ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Gas Chromatography Mass Spectrometry ◽  
Resource Limited ◽  
Short Period ◽  
Reaction Gas Chromatography ◽  
Lateral Flow Assays

Lateral flow assays (lateral flow immunoassays and nucleic acid lateral flow assays) have experienced a great boom in a wide variety of early diagnostic and screening applications. As opposed to conventional examinations (High Performance Liquid Chromatography, Polymerase Chain Reaction, Gas chromatography-Mass Spectrometry, etc.), they obtain the results of a sample’s analysis within a short period. In resource-limited areas, these tests must be simple, reliable, and inexpensive. In this review, we outline the production process of antibodies against drugs of abuse (such as heroin, amphetamine, benzodiazepines, cannabis, etc.), used in lateral flow immunoassays as revelation or detection molecules, with a focus on the components, the principles, the formats, and the mechanisms of reaction of these assays. Further, we report the monoclonal antibody advantages over the polyclonal ones used against drugs of abuse. The perspective on aptamer use for lateral flow assay development was also discussed as a possible alternative to antibodies in view of improving the limit of detection, sensitivity, and specificity of lateral flow assays.

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

Inductively coupled plasma optical emission spectroscopy as a tool for evaluating lateral flow assays

2021 ◽  
Author(s):  
Jenna M. DeSousa ◽  
Micaella Z. Jorge ◽  
Hayley B. Lindsay ◽  
Frederick R. Haselton ◽  
David W. Wright ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Emission Spectroscopy ◽  
Optical Emission Spectroscopy ◽  
Optical Emission ◽  
Lateral Flow ◽  
Gold Content ◽  
Icp Oes ◽  
Inductively Coupled ◽  
Lateral Flow Assays ◽  
Plasma Optical Emission Spectroscopy

This work demonstrates the first use of ICP-OES to quantitatively analyze gold content on lateral flow assays.

Gold nanorods-based lateral flow biosensors for sensitive detection of nucleic acids

2021 ◽  
Vol 188 (4) ◽  
Author(s):  
Qingcai Yu ◽  
Jing Zhang ◽  
Wanwei Qiu ◽  
Kun Li ◽  
Lisheng Qian ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Gold Nanorods ◽  
Lateral Flow ◽  
Sensitive Detection

Functional nucleic acids tailoring and its application

2019 ◽  
Vol 118 ◽  
pp. 138-157 ◽  
Author(s):  
Xingning Xiao ◽  
Longjiao Zhu ◽  
Wanchong He ◽  
Yunbo Luo ◽  
Wentao Xu
Keyword(s):  
Nucleic Acids ◽  
Functional Nucleic Acids

Functional nucleic acids for cancer theranostics

2020 ◽  
Vol 403 ◽  
pp. 213080 ◽  
Author(s):  
Tianhuan Peng ◽  
Zhengyu Deng ◽  
Jiaxuan He ◽  
Yingying Li ◽  
Yan Tan ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Cancer Theranostics ◽  
Functional Nucleic Acids
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