scholarly journals Low-cost 3D-printed inverted microscope to detect Mycobacterium tuberculosis in a MODS culture

Tuberculosis ◽  
2021 ◽  
pp. 102158
Author(s):  
Mario Salguedo ◽  
Guillermo Zarate ◽  
Jorge Coronel ◽  
Germán Comina ◽  
Robert H. Gilman ◽  
...  
2020 ◽  
Author(s):  
Mario Salguedo ◽  
Guillermo Zarate ◽  
Robert H. Gilman ◽  
Germán Comina ◽  
Jorge Coronel ◽  
...  

AbstractBackgroundThe MODS is an important assay for early diagnosis of tuberculosis and drug susceptibility. MODS is based in the microscopic observation, underneath, of the characteristic cords of Mycobacterium tuberculosis colonies grown in liquid media. An inverted optical microscope is required to observe and interpret MODS cultures. Unfortunately, the cost of commercial inverted microscopes is not affordable in low resource settings in developing countries.MethodologyTo perform a diagnosis of tuberculosis using the MODS assay, images with modest quality are enough for proper interpretation. Therefore, the use of a high cost commercial inverted optical microscope is not indispensable. In this study, we designed a prototype of an optical inverted microscope created with a 3D printer and based on a smartphone. The system was evaluated by comparison of manual interpretations of 226 TB positive MODS culture images and 207 negative MODS culture images.SignificanceThe prototype resulted in a low-cost inverted optical microscope, with simple functioning, and whose parts have been manufactured using 3D printing techniques. The quality of the images was good enough and achieved a 100% concordance between the manual inspection with the developed microscope, and the standard diagnostics of MODS.


2019 ◽  
Author(s):  
Alperen Guver ◽  
Nafetalai Fifita ◽  
Peker Milas ◽  
Michael Straker ◽  
Michael Guy ◽  
...  

AbstractA low-cost Scanning Electrochemical Microscope (SECM) was built with a 0.6 pA current measurement capability potentiostat and submicron resolution motorized stage, using open source software and hardware tools. The high performance potentiostat with a Python graphical user interface was built based on an open source project. Arduino boards, stepper motors, a manual XY micromanipulator stage, 3D printed couplers and gears were used in building the motorized stage. An open source motor control software was used for moving the motorized stage with high precision. An inverted microscope was utilized for viewing a standard microelectrode while scanning. The setup was tested in the formation of a map of electrochemical signals from an array of pores on a parafilm membrane. As the setup will be used in future biosensing experiments, DNA hybridization detection experiments were also performed with the setup.


PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9577 ◽  
Author(s):  
Mirko Zimic ◽  
Abner Velazco ◽  
Germán Comina ◽  
Jorge Coronel ◽  
Patricia Fuentes ◽  
...  

2020 ◽  
Author(s):  
Merel van der Stelt ◽  
Martin P. Grobusch ◽  
Abdul R. Koroma ◽  
Marco Papenburg ◽  
Ismaila Kebbie ◽  
...  

Sensors ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 1977
Author(s):  
Ricardo Oliveira ◽  
Liliana M. Sousa ◽  
Ana M. Rocha ◽  
Rogério Nogueira ◽  
Lúcia Bilro

In this work, we demonstrate for the first time the capability to inscribe long-period gratings (LPGs) with UV radiation using simple and low cost amplitude masks fabricated with a consumer grade 3D printer. The spectrum obtained for a grating with 690 µm period and 38 mm length presented good quality, showing sharp resonances (i.e., 3 dB bandwidth < 3 nm), low out-of-band loss (~0.2 dB), and dip losses up to 18 dB. Furthermore, the capability to select the resonance wavelength has been demonstrated using different amplitude mask periods. The customization of the masks makes it possible to fabricate gratings with complex structures. Additionally, the simplicity in 3D printing an amplitude mask solves the problem of the lack of amplitude masks on the market and avoids the use of high resolution motorized stages, as is the case of the point-by-point technique. Finally, the 3D printed masks were also used to induce LPGs using the mechanical pressing method. Due to the better resolution of these masks compared to ones described on the state of the art, we were able to induce gratings with higher quality, such as low out-of-band loss (0.6 dB), reduced spectral ripples, and narrow bandwidths (~3 nm).


HardwareX ◽  
2021 ◽  
pp. e00214
Author(s):  
David T. McCarthy ◽  
Baiqian Shi ◽  
Miao Wang ◽  
Stephen Catsamas
Keyword(s):  
Low Cost ◽  

Author(s):  
Romain Nicot ◽  
Edwige Hurteloup ◽  
Sébastien Joachim ◽  
Charles Druelle ◽  
Jean-Marc Levaillant

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2518
Author(s):  
Nunzio Cennamo ◽  
Lorena Saitta ◽  
Claudio Tosto ◽  
Francesco Arcadio ◽  
Luigi Zeni ◽  
...  

In this work, a novel approach to realize a plasmonic sensor is presented. The proposed optical sensor device is designed, manufactured, and experimentally tested. Two photo-curable resins are used to 3D print a surface plasmon resonance (SPR) sensor. Both numerical and experimental analyses are presented in the paper. The numerical and experimental results confirm that the 3D printed SPR sensor presents performances, in term of figure of merit (FOM), very similar to other SPR sensors made using plastic optical fibers (POFs). For the 3D printed sensor, the measured FOM is 13.6 versus 13.4 for the SPR-POF configuration. The cost analysis shows that the 3D printed SPR sensor can be manufactured at low cost (∼15 €) that is competitive with traditional sensors. The approach presented here allows to realize an innovative SPR sensor showing low-cost, 3D-printing manufacturing free design and the feasibility to be integrated with other optical devices on the same plastic planar support, thus opening undisclosed future for the optical sensor systems.


Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 572
Author(s):  
Mads Jochumsen ◽  
Taha Al Muhammadee Janjua ◽  
Juan Carlos Arceo ◽  
Jimmy Lauber ◽  
Emilie Simoneau Buessinger ◽  
...  

Brain-computer interfaces (BCIs) have been proven to be useful for stroke rehabilitation, but there are a number of factors that impede the use of this technology in rehabilitation clinics and in home-use, the major factors including the usability and costs of the BCI system. The aims of this study were to develop a cheap 3D-printed wrist exoskeleton that can be controlled by a cheap open source BCI (OpenViBE), and to determine if training with such a setup could induce neural plasticity. Eleven healthy volunteers imagined wrist extensions, which were detected from single-trial electroencephalography (EEG), and in response to this, the wrist exoskeleton replicated the intended movement. Motor-evoked potentials (MEPs) elicited using transcranial magnetic stimulation were measured before, immediately after, and 30 min after BCI training with the exoskeleton. The BCI system had a true positive rate of 86 ± 12% with 1.20 ± 0.57 false detections per minute. Compared to the measurement before the BCI training, the MEPs increased by 35 ± 60% immediately after and 67 ± 60% 30 min after the BCI training. There was no association between the BCI performance and the induction of plasticity. In conclusion, it is possible to detect imaginary movements using an open-source BCI setup and control a cheap 3D-printed exoskeleton that when combined with the BCI can induce neural plasticity. These findings may promote the availability of BCI technology for rehabilitation clinics and home-use. However, the usability must be improved, and further tests are needed with stroke patients.


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