Effect of Low-Intensity Pulsed Ultrasound on Orthodontically Induced Root Resorption in Beagle Dogs

ABSTRACT Objectives: To evaluate the effects of low-intensity pulsed ultrasound (LIPUS) on orthodontically induced tooth root resorption caused by torque in human subjects. Materials and Methods: Ten healthy patients (12–35 years of age) who required extraction of all first premolars as a part of their routine orthodontic treatment were recruited. A 15° twist was applied in the arch wire using 0.019 × 0.025-inch TMA in a 0.022-inch bracket system (Synergy R) that produced a buccal root torque of approximately 5 N/mm at the bracket level. Using a split mouth design, randomization, and blinding, one side of the arch received LIPUS for 20 minutes per day for 4 weeks at an incident intensity of 30 mW/cm2 of the transducers’ surface area. The other side served as a self-control, which received a sham transducer. After 4 weeks, all first premolars were extracted and micro–computed tomographic analysis was performed on these extracted teeth. A linear mixed-model statistical analysis was used. Results: LIPUS-treated teeth showed significantly less total volume of resorption lacunae compared to control teeth by a mean difference of (0.54 ± 0.09 mm3) (P < .001) and percentage of root resorption by a mean difference of (0.33 ± 0.05 mm3) (P < .001). In addition, significantly fewer resorption lacunae were found on all root surfaces in the LIPUS group compared to the control except in the instance of the distal surface. Limitations: This study was performed on limited number of cases during a 4-week period. Conclusions: LIPUS minimizes root resorption when applied during torque tooth movement over a 4-week period.

Download Full-text

Effect of Low Intensity Pulsed Ultrasound (LIPUS) on Tooth Movement and Root Resorption: A Prospective Multi-Center Randomized Controlled Trial

Journal of Clinical Medicine ◽

10.3390/jcm9030804 ◽

2020 ◽

Vol 9 (3) ◽

pp. 804 ◽

Cited By ~ 4

Author(s):

Tarek El-Bialy ◽

Khaled Farouk ◽

Terry D. Carlyle ◽

William Wiltshire ◽

Robert Drummond ◽

...

Keyword(s):

Tooth Movement ◽

Root Resorption ◽

Controlled Trial ◽

Control Group ◽

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity ◽

Control Side ◽

Before And After ◽

Group 1

The aim of this study was to evaluate the possible effect of low intensity pulsed ultrasound (LIPUS) on tooth movement and root resorption in orthodontic patients. Twenty-one patients were included in a split-mouth study design (group 1). Ten additional patients were included with no LIPUS device being used and this group was used as the negative control group (group 2). Group 1 patients were given LIPUS devices that were randomly assigned to right or left side on upper or lower arches. LIPUS was applied to the assigned side that was obtained by randomization, using transducers that produce ultrasound with a pulse frequency of 1.5 MHz, a pulse repetition rate of 1 kHz, and average output intensity of 30 mW/cm2. Cone-beam computed tomography (CBCT) images were taken before and after treatment. The extraction space dimensions were measured every four weeks and root lengths of canines were measured before and after treatment. The data were analyzed using paired t-test. The study outcome showed that the mean rate of tooth movement in LIPUS side was 0.266 ± 0.092 mm/week and on the control side was 0.232 ± 0.085 mm/week and the difference was statistically significant. LIPUS increased the rate of tooth movement by an average of 29%. For orthodontic root resorption, the LIPUS side (0.0092 ± 0.022 mm/week) showed a statistically significant decrease as compared to control side (0.0223 ± 0.022 mm/week). The LIPUS application accelerated tooth movement and minimized orthodontically induced tooth root resorption at the same time.

Download Full-text

Effects of Low-Intensity Pulsed Ultrasound on Orthodontic Tooth Movement and Orthodontically Induced Inflammatory Root Resorption in Ovariectomized Osteoporotic Rats

Ultrasound in Medicine & Biology ◽

10.1016/j.ultrasmedbio.2015.11.018 ◽

2016 ◽

Vol 42 (3) ◽

pp. 808-814 ◽

Cited By ~ 5

Author(s):

Feras Y. Dahhas ◽

Tarek El-Bialy ◽

Ahmed R. Afify ◽

Ali H. Hassan

Keyword(s):

Tooth Movement ◽

Root Resorption ◽

Orthodontic Tooth Movement ◽

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity

Download Full-text

Low-intensity pulsed ultrasound attenuates replacement root resorption of avulsed teeth stored in dry condition in dogs

Scientific Reports ◽

10.1038/s41598-021-92471-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Saemi Seong ◽

Dohyun Kim ◽

Dasun Lee ◽

Hyung-Ryong Kim ◽

Yooseok Shin

Keyword(s):

Computed Tomography ◽

Salt Solution ◽

Root Resorption ◽

Storage Conditions ◽

Histological Findings ◽

Micro Computed Tomography ◽

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity ◽

Computed Tomography Images

AbstractThis study aimed to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on replacement root resorption after replantation of avulsed teeth stored in a dry condition in dogs. A total of 73 premolar roots from four male mongrel dogs were intentionally avulsed with forceps and divided into four groups—HN, HL, DN, and DL—according to storage conditions and whether or not they received LIPUS treatment. Thirty-eight roots were kept in Hanks’ Balanced Salt Solution for 30 min (HN and HL groups), whereas the remaining 35 roots were left to dry in the air for an hour (DN and DL groups) prior to replantation. Following replantation, the roots in the HL and DL groups (21 and 18 roots, respectively) received a 20-min daily LIPUS treatment for 2 weeks. The animals were euthanized 4 weeks after the operation. Micro-computed tomography images were acquired for each root and the amount of replacement root resorption was measured three-dimensionally. Histological assessments were also carried out. There was significantly less replacement root resorption for the roots in the DL group compared to the DN group (p < 0.01). Histological findings in the DN group demonstrated evident replacement root resorption, whereas the DL group revealed less severe resorption compared to the DN group. Within the limitations, these results suggest that LIPUS could attenuate the replacement resorption of avulsed teeth stored in a dry condition, thereby improving their prognosis.

Download Full-text

Faculty Opinions recommendation of Effects and Mechanisms of Low-Intensity Pulsed Ultrasound for Chronic Prostatitis and Chronic Pelvic Pain Syndrome.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726483280.793536472 ◽

2017 ◽

Author(s):

Jaspreet Sandhu

Keyword(s):

Pelvic Pain ◽

Chronic Prostatitis ◽

Chronic Pelvic Pain ◽

Chronic Pelvic Pain Syndrome ◽

Pain Syndrome ◽

Pelvic Pain Syndrome ◽

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity

Download Full-text

Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

Bioactive Materials ◽

10.1016/j.bioactmat.2021.04.016 ◽

2021 ◽

Vol 6 (11) ◽

pp. 4073-4082

Author(s):

Kunzhan Cai ◽

Yilai Jiao ◽

Quan Quan ◽

Yulin Hao ◽

Jie Liu ◽

...

Keyword(s):

Pulsed Ultrasound ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity

Download Full-text

Development and Validation of Low-Intensity Pulsed Ultrasound Systems for Highly Controlled in vitro Cell Stimulation

Ultrasonics ◽

10.1016/j.ultras.2021.106495 ◽

2021 ◽

pp. 106495

Author(s):

F. Fontana ◽

F. Iberite ◽

A. Cafarelli ◽

A. Aliperta ◽

G. Baldi ◽

...

Keyword(s):

Pulsed Ultrasound ◽

Cell Stimulation ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity ◽

Development And Validation

Download Full-text

Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing

Scientific Reports ◽

10.1038/s41598-021-89672-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Tatsuya Shimizu ◽

Naomasa Fujita ◽

Kiyomi Tsuji-Tamura ◽

Yoshimasa Kitagawa ◽

Toshiaki Fujisawa ◽

...

Keyword(s):

Fracture Healing ◽

Transcriptional Control ◽

Target Genes ◽

Pulsed Ultrasound ◽

Activated T Cells ◽

In Vivo Models ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity ◽

Ultrasound Stimulation

AbstractUltrasound stimulation is a type of mechanical stress, and low-intensity pulsed ultrasound (LIPUS) devices have been used clinically to promote fracture healing. However, it remains unclear which skeletal cells, in particular osteocytes or osteoblasts, primarily respond to LIPUS stimulation and how they contribute to fracture healing. To examine this, we utilized medaka, whose bone lacks osteocytes, and zebrafish, whose bone has osteocytes, as in vivo models. Fracture healing was accelerated by ultrasound stimulation in zebrafish, but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocytes, we performed RNA sequencing of a murine osteocytic cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation, and functional cluster analysis identified among them several molecular signatures related to immunity, secretion, and transcription. Notably, most of the isolated transcription-related genes were also modulated by LIPUS in vivo in zebrafish. However, expression levels of early growth response protein 1 and 2 (Egr1, 2), JunB, forkhead box Q1 (FoxQ1), and nuclear factor of activated T cells c1 (NFATc1) were not altered by LIPUS in medaka, suggesting that these genes are key transcriptional regulators of LIPUS-dependent fracture healing via osteocytes. We therefore show that bone-embedded osteocytes are necessary for LIPUS-induced promotion of fracture healing via transcriptional control of target genes, which presumably activates neighboring cells involved in fracture healing processes.

Download Full-text