Comparison of hemagglutination inhibition and microbead array assays for the measurement of influenza antibody levels in HIV-infected adults

Vaccine ◽  
2016 ◽  
Vol 34 (31) ◽  
pp. 3584-3591
Author(s):  
Anona Thorne ◽  
Fahad Chowdhury ◽  
Joel Singer ◽  
Yoav Keynan ◽  
Keith R. Fowke ◽  
...  
Keyword(s):  
Hemagglutination Inhibition ◽  
Antibody Levels ◽  
Microbead Array

scholarly journals Preexisting Immunity, More Than Aging, Influences Influenza Vaccine Responses

2015 ◽  
Vol 2 (2) ◽  
Author(s):  
Adrian J. Reber ◽  
Jin Hyang Kim ◽  
Renata Biber ◽  
H. Keipp Talbot ◽  
Laura A. Coleman ◽  
...  
Keyword(s):  
Older Adults ◽  
B Cells ◽  
Immune Responses ◽  
Influenza Vaccine ◽  
Hemagglutination Inhibition ◽  
Negative Impact ◽  
Memory B Cells ◽  
Vaccine Response ◽  
Vaccine Responses ◽  
Antibody Levels

Abstract Background.  Influenza disproportionately impacts older adults while current vaccines have reduced effectiveness in the older population. Methods.  We conducted a comprehensive evaluation of cellular and humoral immune responses of adults aged 50 years and older to the 2008–2009 seasonal trivalent inactivated influenza vaccine and assessed factors influencing vaccine response. Results.  Vaccination increased hemagglutination inhibition and neutralizing antibody; however, 66.3% of subjects did not reach hemagglutination inhibition titers ≥ 40 for H1N1, compared with 22.5% for H3N2. Increasing age had a minor negative impact on antibody responses, whereas prevaccination titers were the best predictors of postvaccination antibody levels. Preexisting memory B cells declined with age, especially for H3N2. However, older adults still demonstrated a significant increase in antigen-specific IgG+ and IgA+ memory B cells postvaccination. Despite reduced frequency of preexisting memory B cells associated with advanced age, fold-rise in memory B cell frequency in subjects 60+ was comparable to subjects age 50–59. Conclusions.  Older adults mounted statistically significant humoral and cell-mediated immune responses, but many failed to reach hemagglutination inhibition titers ≥40, especially for H1N1. Although age had a modest negative effect on vaccine responses, prevaccination titers were the best predictor of postvaccination antibody levels, irrespective of age.

scholarly journals Influenza Virus Hemagglutinin Stalk-Specific Antibodies in Human Serum are a Surrogate Marker for In Vivo Protection in a Serum Transfer Mouse Challenge Model

mBio ◽  
2017 ◽  
Vol 8 (5) ◽  
Author(s):  
Henning Jacobsen ◽  
Madhusudan Rajendran ◽  
Angela Choi ◽  
Haakon Sjursen ◽  
Karl A. Brokstad ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Human Serum ◽  
Hemagglutination Inhibition ◽  
Surrogate Marker ◽  
Virus Challenge ◽  
Vaccination Trial ◽  
Serum Transfer ◽  
Stalk Domain ◽  
Antibody Levels

ABSTRACT The immunogenicity of current influenza virus vaccines is assessed by measuring an increase of influenza virus-specific antibodies in a hemagglutination inhibition assay. This method exclusively measures antibodies against the hemagglutinin head domain. While this domain is immunodominant, it has been shown that hemagglutination inhibition titers do not always accurately predict protection from disease. In addition, several novel influenza virus vaccines that are currently under development do not target the hemagglutinin head domain, but rather more conserved sites, including the hemagglutinin stalk. Importantly, antibodies against the hemagglutinin stalk do not show activity in hemagglutination inhibition assays and will require different methods for quantification. In this study, we tested human serum samples from a seasonal influenza virus vaccination trial and an avian H5N1 virus vaccination trial for antibody activities in multiple types of assays, including binding assays and also functional assays. We then performed serum transfer experiments in mice which then received an H1N1 virus challenge to assess the in vivo protective effects of the antibodies. We found that hemagglutinin-specific antibody levels measured in an enzyme-linked immunosorbent assay (ELISA) correlated well with protection from weight loss in mice. In addition, we found that weight loss was also inversely correlated with the level of serum antibody-dependent cellular cytotoxicity (ADCC) as measured in a reporter assay. These findings indicate that protection is in part conferred by Fc-dependent mechanisms. In conclusion, ELISAs can be used to measure hemagglutinin-specific antibody levels that could serve as a surrogate marker of protection for universal influenza virus vaccines. IMPORTANCE Influenza viruses are a serious concern for public health and cause a large number of deaths worldwide every year. Current influenza virus vaccines can confer protection from disease, but they often show low efficacy due to the ever-changing nature of the viruses. Novel vaccination approaches target conserved epitopes of the virus, including the hemagglutinin stalk domain, to elicit universally protective antibodies that also bind to mutated viruses or new subtypes of viruses. Importantly, the hemagglutination inhibition assay—the only assay that has been accepted as a correlate of protection by regulatory authorities—cannot measure antibodies against the hemagglutinin stalk domain. Therefore, novel correlates of protection and assays to measure vaccine immunogenicity need to be developed. In this study, we correlated the results from multiple assays with protection in mice after transfer of human serum and a lethal virus challenge to investigate potential novel serological surrogate markers for protection.

scholarly journals Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach

2020 ◽  
Author(s):  
Chanakan Areewong ◽  
Amarin Rittipornlertrak ◽  
Boondarika Nambooppha ◽  
Itsarapan Fhaikrue ◽  
Tawatchai Singhla ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Hemagglutination Inhibition ◽  
Indirect Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Panthera Tigris ◽  
Serum Samples ◽  
Feline Panleukopenia Virus ◽  
Elisa Testing ◽  
Antibody Levels

Abstract Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae including tigers (Panthera tigris). Vaccination against FPV among wild felid species has long been practiced in zoos worldwide. However, few studies have assessed tiger immune response post-vaccination due to the absence of a serological diagnostic tool. To address these limitations, this study aimed to develop an in-house indirect enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody levels against the feline panleukopenia vaccine by employing the synthesized subunit capsid protein VP2. An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced in this study and employed in the assay. It was then compared to a commercial HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity were evaluated using the Bayesian model with conditional dependence being assumed between both HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) tests. Results: The posterior estimates for sensitivity and specificity of two indirect ELISA HRP-conjugated antibodies were higher than those of the HI test. The sensitivity and specificity of indirect ELISA for HRP-anti-tiger IgG and HRP-anti-cat IgG were 86.5%, 57.2% and 86.7%, 64.6%, respectively, while the results of the HI test were 79.1 and 54.1%. In applications, 89.6% (198/221) of tiger serum samples were determined to be seropositive by indirect ELISA testing. Conclusion: The results support evidence that an in-house indirect ELISA developed in this study could be used as a serological tool for the effective detection of tiger antibodies.

scholarly journals One-Step Immunochromatography Assay Kit for Detecting Antibodies to Canine Parvovirus

2006 ◽  
Vol 13 (4) ◽  
pp. 520-524 ◽  
Author(s):  
Jin-Sik Oh ◽  
Gun-Woo Ha ◽  
Young-Shik Cho ◽  
Min-Jae Kim ◽  
Dong-Jun An ◽  
...  
Keyword(s):  
Gold Standard ◽  
Hemagglutination Inhibition ◽  
Reliable Method ◽  
Vaccination Schedule ◽  
Single Step ◽  
Canine Parvovirus ◽  
Maternal Antibodies ◽  
Special Equipment ◽  
One Step ◽  
Antibody Levels

ABSTRACT This study was performed to determine the feasibility of using whole serum to detect antibodies to canine parvovirus (CPV) under nonlaboratory conditions and to evaluate the performance characteristics of an immunochromatography assay kit. Precise detection of levels of antibody against CPV in puppies can be used to determine a vaccination schedule, because maternal antibodies frequently result in the failure of protective vaccination, and can also be used to determine the antibody levels of infected puppies. Several methods for the titration of CPV antibodies have been reported, including the hemagglutination inhibition (HI) assay, which is considered the “gold standard.” These methods, however, require intricate and time-consuming procedures. In this study, a total of 386 serum specimens were tested. Compared to the HI assay, the rapid assay had a 97.1% sensitivity and a 76.6% specificity (with a cutoff HI titer of 1:80). This single-step assay could be performed rapidly and easily without special equipment. The kit provides a reliable method for detection of anti-CPV antibody where laboratory support and personnel are limited.

scholarly journals Serum Hemagglutination Inhibition Activity Correlates with Protection from Gastroenteritis in Persons Infected with Norwalk Virus

2011 ◽  
Vol 19 (2) ◽  
pp. 284-287 ◽  
Author(s):  
Rita Czakó ◽  
Robert L. Atmar ◽  
Antone R. Opekun ◽  
Mark A. Gilger ◽  
David Y. Graham ◽  
...  
Keyword(s):  
Blood Group ◽  
Hemagglutination Inhibition ◽  
Serum Antibody ◽  
Blood Group Antigen ◽  
Antibody Responses ◽  
Norwalk Virus ◽  
Inhibition Activity ◽  
Whitney Test ◽  
Antibody Levels ◽  
Blocking Assay

ABSTRACTA hemagglutination inhibition (HAI) assay to assess serum antibody responses following Norwalk virus (NV) infection was developed. HAI activity increased significantly in individuals experimentally infected with NV (n= 18) and correlated with antibody levels measured in a histo-blood group antigen (HBGA) blocking assay. Prechallenge HAI antibody levels also correlated with protection from the development of gastroenteritis (Mann-Whitney test,P= 0.02). The HAI assay is another assay suitable for the detection of antibody that correlates with protection from Norwalk virus-associated disease.

scholarly journals Single-radial hemolysis as a cost-effective determinant of Rubella antibody status

1979 ◽  
Vol 9 (1) ◽  
pp. 115-119
Author(s):  
J M Forger ◽  
R F Gilfillan
Keyword(s):  
General Population ◽  
Hemagglutination Inhibition ◽  
Cost Effective ◽  
Antibody Levels ◽  
Made In ◽  
Rubella Antibody

Single-radial hemolysis was examined for sensitivity, reliability, and cost for determination of rubella antibody levels in the general population. Results obtained with single-radial hemolysis plates made in this laboratory, and those under development by a commercial manufacturer, were compared with those obtained by the hemagglutination inhibition method normally used for rubella antibody determinations. The results suggest that single-radial hemolysis is comparable to hemagglutination inhibition with respect to sensitivity and reliability; it is superior to hemagglutination inhibition in terms of ease of manipulation and economy of use.

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