Single oral immunization of an attenuated Salmonella Gallinarium formulation consisting of equal quantities of strains secreting H9N2 hemagglutinin-HA1, HA2, and M2eCD154 induces significant protection against H9N2 and partial protection against Salmonella Gallinarium challenge in chickens

2021 ◽  
Vol 240 ◽  
pp. 110318
Author(s):  
Amal Senevirathne ◽  
Chamith Hewawaduge ◽  
Sungwoo Park ◽  
Vijayakumar Jawalagatti ◽  
Chonghan Kim ◽  
...  
Keyword(s):  
Oral Immunization ◽  
Significant Protection ◽  
Partial Protection

scholarly journals Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen ofHaemonchus contortus(rHcp26/23)

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Leticia García-Coiradas ◽  
Francisco Angulo-Cubillán ◽  
Basilio Valladares ◽  
Enrique Martínez ◽  
Concepción de la Fuente ◽  
...  
Keyword(s):  
Recombinant Dna ◽  
Soluble Fraction ◽  
Parasitic Disease ◽  
Significant Protection ◽  
Recombinant Dna Technology ◽  
Specific Antibody Response ◽  
Partial Protection ◽  
Synthetic Protein ◽  
Immunological Assays ◽  
And Control

Haemonchosis, caused by the abomasal nematodeHaemonchus contortus, is a common parasitic disease of sheep. Our previous results showed that a soluble fraction from adult stages of the nematode (p26/23) induced partial protection against challenge. Recombinant DNA technology was applied to obtain a synthetic protein (rHcp26/23). Immunological assays (ELISA, Western blotting, and immunolocalization), using sera from lambs immunized with p26/23, confirmed the identity of the recombinant protein and demonstrated that the synthetic protein is equivalent to the purified protein employed in the previous immunoprophylaxis studies. Vaccination of lambs with 300 μgof rHcp26/23 and Freund's adjuvant elicited a notable specific antibody response. Immunization did not induce any significant protection after challenge with 16000 infective larvae ofH. contortus, and comparable values for parasite faecal egg output, packed cell volume, and abomasal parasite burdens were found in vaccinated and control animals.

scholarly journals Partial Protection against Brucella Infection in Mice by Immunization with Nonpathogenic Alphaproteobacteria

2007 ◽  
Vol 14 (10) ◽  
pp. 1296-1301 ◽  
Author(s):  
M. Victoria Delpino ◽  
Silvia M. Estein ◽  
Carlos A. Fossati ◽  
Pablo C. Baldi
Keyword(s):  
Brucella Abortus ◽  
Sinorhizobium Meliloti ◽  
Brucella Melitensis ◽  
Oral Immunization ◽  
Positive Control ◽  
Oral Route ◽  
Partial Protection ◽  
Mesorhizobium Loti ◽  
The Moment ◽  
Phosphate Buffered Saline

ABSTRACT Previous findings indicate that Brucella antigens and those from nonpathogenic alphaproteobacteria (NPAP) are cross-recognized by the immune system. We hypothesized that immunization with NPAP would protect mice from Brucella infection. Mice were immunized subcutaneously with heat-killed Ochrobactrum anthropi, Sinorhizobium meliloti, Mesorhizobium loti, Agrobacterium tumefaciens, or Brucella melitensis H38 (standard positive control) before intravenous challenge with Brucella abortus 2308. Cross-reacting serum antibodies against Brucella antigens were detected at the moment of challenge in all NPAP-immunized mice. Thirty days after B. abortus challenge, splenic CFU counts were significantly lower in mice immunized with O. anthropi, M. loti, and B. melitensis H38 than in the phosphate-buffered saline controls (protection levels were 0.80, 0.66, and 1.99 log units, respectively). In mice immunized intraperitoneally with cytosoluble extracts from NPAP or Brucella abortus, protection levels were 1.58 for the latter, 0.63 for O. anthropi, and 0.40 for M. loti. To test whether the use of live NPAP would increase protection further, mice were both immunized and challenged by the oral route. Immunization with NPAP induced a significant increase in serum immunoglobulin G (IgG), but not serum or fecal IgA, against Brucella antigens. After challenge, anti-Brucella IgA increased significantly in the sera and feces of mice orally immunized with O. anthropi. For all NPAP, protection levels were higher than those obtained with systemic immunizations but were lower than those obtained by oral immunization with heat-killed B. abortus. These results show that immunization with NPAP, especially O. anthropi, confers partial protection against Brucella challenge. However, such protection is lower than that conferred by immunization with whole Brucella or its cytosoluble fraction.

Influence of thromboxane inhibition on the severity of myocardial ischemia in cats

1980 ◽  
Vol 58 (3) ◽  
pp. 294-300 ◽  
Author(s):  
Edward F. Smith III ◽  
Allan M. Lefer ◽  
J. Bryan Smith
Keyword(s):  
Myocardial Ischemia ◽  
Nitrogen Content ◽  
Creatine Phosphokinase ◽  
Amino Nitrogen ◽  
Myocardial Tissue ◽  
Significant Protection ◽  
Partial Protection ◽  
Content Assessment ◽  
Degree Of Protection ◽  
Acute Mi

The effects of thromboxane (Tx) inhibition or arachidonic acid (AA) infusion were studied in anesthetized cats during acute myocardial ischemia (MI). AA (7.2 mg kg−1 h−1) or imidazole (25 mg kg−1 h−1) infusions were initiated 30 min after occlusion of the left anterior descending coronary artery. Assessment of the degree of protection of the ischemic myocardium was made by measurement of S-T segment elevation, plasma and myocardial creatine phosphokinase (CPK) activities, and myocardial amino-nitrogen content. Assessment of Tx inhibition was performed by radioimmunoassay. Administration of imidazole inhibited the sevenfold increase in plasma thromboxane B2 (TxB2) levels occurring in MI (p < 0.001 at 2–5 h), markedly decreased S-T segment elevations at 2–5 h (p < 0.025), significantly prevented the elevation in plasma CPK (p < 0.05, at 4 and 5 h), and significantly reduced the loss in myocardial CPK activity and myocardial amino-nitrogen content. AA infusion also reduced the increase in TxB2 post-MI, significantly decreased (p < 0.025) S-T segment elevations at 2 5 h, caused a decrease in plasma CPK levels (p < 0.05 at 5 h), but did not prevent loss of myocardial CPK or amino-nitrogen. In summary, the administration of imidazole resulted in significant protection of the myocardium in all indices of ischemic damage measured, while AA infusion resulted in only a partial protection. The mechanism of the imidazole protection of ischemic myocardial tissue appears to be via inhibition of Tx synthesis although we cannot exclude a hemodynamic or cytoprotective mechanism. These results suggest that specific inhibition of Tx formation is beneficial during acute MI.

Applying a Significant Protection Layer on Double Ex Situ Lift-out TEM Specimens to Protect Against Ion Beam Impact

2019 ◽  
Author(s):  
Chun-Hung Lin ◽  
Hsin-Cheng Hsu ◽  
Tsung-Yi Lin ◽  
Ru-Hui Lin ◽  
I-An Chen ◽  
...  
Keyword(s):  
Ion Beam ◽  
Ex Situ ◽  
Preliminary Treatment ◽  
Significant Protection ◽  
Damage Layer ◽  
Protection Method ◽  
Protection Layer ◽  
Tem Lamella ◽  
Beam Damage

Abstract Protection layers on double ex situ lift-out TEM specimens were investigate in this paper and two protection layer approaches for double INLO or double EXLO were introduced. The improved protection methods greatly decreased the damage layer on the top surface from 90 nm to 5 nm (or lower) during FIB milling. According to the property of different sample and its preliminary treatment in the FIB, we have the satisfactory approaches to be applied. Using this improved protection method, we demonstrate the structures within the TEM lamella can be observed without ion beam damage/implantation during FIB

METHOD OF DETERMINING THE IMMUNOGENIC ACTIVITY OF LIVE VACCINE AGAINST RABIES IN WILD ANIMALS IN MICE

2020 ◽  
pp. 53-57
Author(s):  
V. A. Babak ◽  
A. A. Gusev ◽  
I. A. Puntus ◽  
A. S. Smailova
Keyword(s):  
Infection Control ◽  
Rabies Virus ◽  
Experimental Infection ◽  
Oral Immunization ◽  
Live Vaccine ◽  
Wild Animals ◽  
Method Of Evaluation

The results of alternative studies on the immunogenic activity of live rabies vaccines for oral immunization of wild carnivorous animals are presented. The method of evaluation of immunogenicity using a model of oral immunization in mice with experimental infection control rabies virus CVS in the dose of 10–100 MLD50/0,03 ml. Once entered immunizing dose for white mice, weighing 12–14 g were 56.200 MLD50, the titers of VNA ranged from 1:6 to 1:16 (3,0–4,0 log2) and above.

Bronchospasmolytic and Adenosine Binding Activity of 8- (Proline / Pyrazole)-Substituted Xanthine Derivatives

2020 ◽  
Vol 17 ◽  
Author(s):  
Sneha Singh ◽  
Madhwi Ojha ◽  
Divya Yadav ◽  
Sonja Kachler ◽  
Karl-Norbert Klotz ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Radioligand Binding ◽  
Binding Activity ◽  
Receptor Subtypes ◽  
Significant Protection ◽  
Binding Studies ◽  
Standard Drug ◽  
Xanthine Derivatives ◽  
Radioligand Binding Studies ◽  
Micromolar Range

Background: ABSTRACT: Background: 8-Phenyltheophylline derivatives exhibit prophylactic effects at a specific dose but do not produce the cardiovascular or emetic side effects associated with xanthines, thereby exhibiting unique characteristics of potential therapeutic importance. Methods: Novel series of 8-(proline/pyrazole)-substituted xanthine analogs has been synthesized. The affinity and selectivity of compounds to adenosine receptors have been assessed by radioligand binding studies. The synthesized compounds also showed good bronchospasmolytic properties (increased onset of bronchospasm; decreased duration of jerks) with 100% survival of animals in comparison to the standard drug. Besides, compound 8f & 9f showed good binding affinity in comparison to other synthesized compounds in the micromolar range. Results: The maximum binding affinity of these compounds was observed for A2B receptors, which is ~ 7 or 10 times higher as compared to A1, A2A and A3 receptors. The newly synthesized derivatives 8f, 9a-f, 17g-m, and 18g-m displayed significant protection against histamine aerosol induced bronchospasm in guinea pigs. Conclusion: Newly synthesized proline/pyrazole based xanthines compounds showed a satisfactory binding affinity for adenosine receptor subtypes. Replacement or variation of substituted proline ring with substituted pyrazole scaffold at 8thposition of xanthine moiety resulted in the reduction of adenosine binding affinity and bronchospasmolytic effects.

scholarly journals Vaccination-induced variation in the 140 kD merozoite surface antigen of Plasmodium knowlesi malaria.

1987 ◽  
Vol 165 (2) ◽  
pp. 359-367 ◽  
Author(s):  
F W Klotz ◽  
D E Hudson ◽  
H G Coon ◽  
L H Miller
Keyword(s):  
Monoclonal Antibodies ◽  
Malaria Infection ◽  
Rhesus Monkeys ◽  
Plasmodium Knowlesi ◽  
Rabbit Antiserum ◽  
Antigenic Determinants ◽  
Partial Protection ◽  
Erythrocyte Invasion ◽  
Merozoite Surface Antigen

Immunity to 143/140 kD schizont antigens of a monkey malaria, Plasmodium knowlesi, provides partial protection to lethal malaria infection in rhesus monkeys challenged with uncloned parasites. To determine the capacity of a cloned parasite to generate variants of the 143/140 kD antigens, immunized monkeys were challenged with a clone of P. knowlesi. Parasites recovered 8 d after inoculation with a cloned parasite retained the 143/140 kD antigens. Parasites recovered 30 d after challenge had undergone changes in the 143/140 kD antigens. Antibodies that block erythrocyte invasion in vitro of the inoculum parasites did not inhibit invasion of erythrocytes by two isolates recovered from the immunized monkeys. An isolate from one monkey recovered on day 30 contained clones expressing new 76/72 kD antigens reactive with rabbit antiserum against the 143/140 kD proteins, and other clones expressing no antigens crossreactive with antisera against the 143/140 kD proteins. An isolate from another monkey obtained 59 d after challenge expressed new antigens of 160/155, 115/113, and 87/85 kD. Using monoclonal antibodies, we found that epitopes were lost from the variant proteins, but we were unable to determine whether new epitopes had appeared. We conclude that clones of P. knowlesi can rapidly vary antigenic determinants on the 143/140 kD proteins in animals immunized with these antigens.

scholarly journals Perspectives for improvement of Mycoplasma hyopneumoniae vaccines in pigs

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Dominiek Maes ◽  
Filip Boyen ◽  
Bert Devriendt ◽  
Peter Kuhnert ◽  
Artur Summerfield ◽  
...  
Keyword(s):  
Immune Responses ◽  
Respiratory Infections ◽  
Mycoplasma Hyopneumoniae ◽  
Administration Route ◽  
Limited Effect ◽  
Partial Protection ◽  
Carrier Molecule ◽  
Humoral Responses ◽  
Porcine Respiratory ◽  
Selection Of

AbstractMycoplasma hyopneumoniae (M. hyopneumoniae) is one of the primary agents involved in the porcine respiratory disease complex, economically one of the most important diseases in pigs worldwide. The pathogen adheres to the ciliated epithelium of the trachea, bronchi, and bronchioles, causes damage to the mucosal clearance system, modulates the immune system and renders the animal more susceptible to other respiratory infections. The pathogenesis is very complex and not yet fully understood. Cell-mediated and likely also mucosal humoral responses are considered important for protection, although infected animals are not able to rapidly clear the pathogen from the respiratory tract. Vaccination is frequently practiced worldwide to control M. hyopneumoniae infections and the associated performance losses, animal welfare issues, and treatment costs. Commercial vaccines are mostly bacterins that are administered intramuscularly. However, the commercial vaccines provide only partial protection, they do not prevent infection and have a limited effect on transmission. Therefore, there is a need for novel vaccines that confer a better protection. The present paper gives a short overview of the pathogenesis and immune responses following M. hyopneumoniae infection, outlines the major limitations of the commercial vaccines and reviews the different experimental M. hyopneumoniae vaccines that have been developed and tested in mice and pigs. Most experimental subunit, DNA and vector vaccines are based on the P97 adhesin or other factors that are important for pathogen survival and pathogenesis. Other studies focused on bacterins combined with novel adjuvants. Very few efforts have been directed towards the development of attenuated vaccines, although such vaccines may have great potential. As cell-mediated and likely also humoral mucosal responses are important for protection, new vaccines should aim to target these arms of the immune response. The selection of proper antigens, administration route and type of adjuvant and carrier molecule is essential for success. Also practical aspects, such as cost of the vaccine, ease of production, transport and administration, and possible combination with vaccines against other porcine pathogens, are important. Possible avenues for further research to develop better vaccines and to achieve a more sustainable control of M. hyopneumoniae infections are discussed.

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