Corrigendum to “Influence of operating conditions on the persistence of E. coli, enterococci, Clostridium perfringens and Clostridioides difficile in semi-continuous mesophilic anaerobic reactors” [Waste Manage. 134 (2021) 32–41]

RESUMO Esta pesquisa teve como objetivo avaliar o desempenho da radiação ultravioleta (UV) para a desinfecção de efluente final de estação de tratamento de esgoto (ETE) sanitário municipal, em escala de bancada de laboratório e operação em batelada. Foram analisadas as interferências dos parâmetros operacionais tempo de exposição (s) à radiação e altura de lâmina líquida (cm) do efluente no reator UV. A eficiência do processo de desinfecção foi avaliada empregando os microrganismos indicadores Clostridium perfringens (C. perfringens), colifagos, Escherichia coli (E. coli) e coliformes totais (CT). Após a desinfecção, foram avaliados os fenômenos de recuperação microbiológica fotorreativação e recuperação no escuro para E. coli e CT. Os resultados indicaram efetiva inativação dos microrganismos indicadores à radiação UV no decorrer do tempo de exposição, o que foi comprovado estatisticamente pela ANOVA de medidas repetidas. C. perfringens foi o microrganismo que apresentou a maior resistência à inativação. Nos ensaios de recuperação microbiológica, ambos os mecanismos foram considerados insignificantes, o que foi comprovado estatisticamente pelos testes t de Student (dados paramétricos) e Wilcoxon (dados não paramétricos). Em todas as análises, o nível de significância foi de 5%.

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Growth of Selected Cross-Contaminating Bacterial Pathogens on Beef and Fish at 15 and 35°C+

Journal of Food Protection ◽

10.4315/0362-028x-57.4.337 ◽

1994 ◽

Vol 57 (4) ◽

pp. 337-340 ◽

Cited By ~ 6

Author(s):

AJIBOLA O. FAPOHUNDA ◽

KENNETH W. MCMILLIN ◽

DOUGLAS L. MARSHALL ◽

W. M. WAITES

Keyword(s):

Escherichia Coli ◽

Clostridium Perfringens ◽

Aeromonas Hydrophila ◽

Growth Response ◽

Cross Contamination ◽

Limited Growth ◽

E Coli ◽

Fish Samples ◽

Conditioning Temperature ◽

The Tropics

Isolates of Escherichia coli and Clostridium perfringens from beef and Aeromonas hydrophila from fish were examined for their ability to survive and grow as cross-contaminates on nonnative tissues at simulated ambient (35°C) and aging/conditioning (15°C) temperatures of handling and retailing found in the tropics. Growth of all isolates over a 10-h period was greater (P < 0.05) on their native tissues at both temperatures. The aging/conditioning temperature effectively limited growth of E. coli and A. hydrophila to less than l-logl0 CFU/g and prevented growth of C. perfringens on beef and fish samples. All three isolates demonstrated characteristic mesophilic growth response on both tissues at 35°C during the 10-h retail period. The study suggests that two muscle food products could be jointly handled to efficiently use available storage/haulage capacity in tropical countries. Potential savings in space, labor and energy would be made if cross-contamination between the two products is minimized by available packaging and sanitizing technologies.

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Fecal PCR testing for detection of Clostridium perfringens and Clostridioides difficile toxin genes and other pathogens in foals with diarrhea: 28 cases

Journal of Veterinary Diagnostic Investigation ◽

10.1177/10406387211047529 ◽

2021 ◽

pp. 104063872110475

Author(s):

K. Gary Magdesian ◽

Samantha Barnum ◽

Nicola Pusterla

Keyword(s):

Blood Cell ◽

Clostridium Perfringens ◽

Red Blood Cell ◽

Total Protein ◽

Toxin Gene ◽

Significant Morbidity ◽

Gene Sequences ◽

Clostridioides Difficile ◽

Gastrointestinal Pathogens ◽

Cell Concentrations

Clostridium perfringens and Clostridioides difficile cause significant morbidity and mortality in foals. Antemortem diagnosis of C. perfringens infection has been complicated by a paucity of tests available for toxin detection. Fecal PCR panels have assays for a variety of C. perfringens toxin gene sequences as well as for several other foal gastrointestinal pathogens. We evaluated results of a comprehensive fecal diarrhea PCR panel in 28 foals that had been presented to a referral hospital because of diarrhea. Sixteen (57%) foals were positive for C. perfringens and/or C. difficile toxin gene sequences on fecal PCR, including 3 foals positive for NetF toxin. These foals were younger ( p = 0.0029) and had higher hematocrits ( p = 0.0087), hemoglobin ( p = 0.0067), and red blood cell concentrations ( p = 0.028) than foals with diarrhea that tested negative for clostridial toxins. The foals had lower total protein concentrations ( p = 0.045) and were more likely to have band neutrophils on a CBC ( p = 0.013; OR: 16.2). All 3 foals with NetF toxin gene sequences detected in feces survived to discharge, indicating that diarrhea caused by NetF toxigenic C. perfringens isolates is not uniformly fatal.

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Evaluation of the Microbial Quality of Tajik Sambusa and Control of Clostridium perfringens Germination and Outgrowth by Buffered Sodium Citrate and Potassium Lactate†

Journal of Food Protection ◽

10.4315/0362-028x-71.1.77 ◽

2008 ◽

Vol 71 (1) ◽

pp. 77-82 ◽

Cited By ~ 4

Author(s):

SHAKHLO N. YARBAEVA ◽

PADMANABHA R. VELUGOTI ◽

HARSHAVARDHAN THIPPAREDDI ◽

JULIE A. ALBRECHT

Keyword(s):

Organic Acid ◽

Clostridium Perfringens ◽

Spore Germination ◽

Sodium Citrate ◽

Ground Beef ◽

E Coli ◽

Potassium Lactate ◽

Before And After ◽

Organic Acid Salts ◽

Acid Salts

Clostridium perfringens spore destruction, aerobic plate counts (APCs), and counts of Enterobacteriaceae, coliforms, and Escherichia coli during baking of sambusa (a traditional Tajik food) were evaluated. Control of germination and outgrowth of C. perfringens spores in sambusa during cooling at room or refrigerated temperatures was evaluated using organic acid salts (buffered sodium citrate [Ional] and 1 and 2% potassium lactate, wt/wt). Sambusa were prepared with 40 g of either inoculated or noninoculated meat and baked for 45 min at 180°C. For evaluation of destruction of C. perfringens spores during heating and germination and outgrowth of spores during cooling, ground beef was inoculated and mixed with a three-strain cocktail of C. perfringens spores. Aerobic bacteria, Enterobacteriaceae, coliforms, and E. coli were enumerated in noninoculated sambusa before and after baking and after cooling at room or refrigeration temperatures. After baking, APCs and Enterobacteriaceae and coliform counts were reduced by 4.32, 2.55, and 1.96 log CFU/g, respectively. E. coli counts were below detectable levels in ground beef and sambusa samples. Enterobacteriaceae, coliform, and E. coli counts were below detectable levels (<0.04 log CFU/g) in sambusa after cooling by both methods. Total C. perfringens populations increased (4.67 log CFU/g) during cooling at room temperature, but minimal increases (0.31 log CFU/g) were observed during cooling under refrigeration. Incorporation of 2% (wt/wt) buffered sodium citrate controlled C. perfringens spore germination and outgrowth (0.25 log CFU/g), whereas incorporation of up to 2% (wt/wt) potassium lactate did not prevent C. perfringens spore germination and outgrowth. Incorporation of organic acid salts at appropriate concentrations can prevent germination and outgrowth of C. perfringens in improperly cooled sambusa.

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Isolation of Clostridium perfringens and Clostridioides difficile in diarrheic and nondiarrheic cats

Anaerobe ◽

10.1016/j.anaerobe.2020.102164 ◽

2020 ◽

Vol 62 ◽

pp. 102164 ◽

Cited By ~ 1

Author(s):

Rodrigo Otávio Silveira Silva ◽

Marcio Garcia Ribeiro ◽

Carolina Lechinski de Paula ◽

Isadora Honorato Pires ◽

Carlos Augusto Oliveira Junior ◽

...

Keyword(s):

Clostridium Perfringens ◽

Clostridioides Difficile

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Fecal shedding of Salmonella spp., Clostridium perfringens, and Clostridioides difficile in dogs fed raw meat-based diets in Brazil and their owners’ motivation

PLoS ONE ◽

10.1371/journal.pone.0231275 ◽

2020 ◽

Vol 15 (4) ◽

pp. e0231275

Author(s):

Flavia Mello Viegas ◽

Carolina Pantuzza Ramos ◽

Rafael Gariglio Clark Xavier ◽

Emily Oliveira Lopes ◽

Carlos Augusto Oliveira Júnior ◽

...

Keyword(s):

Clostridium Perfringens ◽

Salmonella Spp ◽

Fecal Shedding ◽

Raw Meat ◽

Clostridioides Difficile

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High Resolution Melt Assays to Detect and Identify Vibrio parahaemolyticus, Bacillus cereus, Escherichia coli, and Clostridioides difficile Bacteria

Microorganisms ◽

10.3390/microorganisms8040561 ◽

2020 ◽

Vol 8 (4) ◽

pp. 561

Author(s):

Allison C. Bender ◽

Jessica A. Faulkner ◽

Katherine Tulimieri ◽

Thomas H. Boise ◽

Kelly M. Elkins

Keyword(s):

Escherichia Coli ◽

High Resolution ◽

Bacillus Cereus ◽

Vibrio Parahaemolyticus ◽

Bacterial Species ◽

Human Pathogens ◽

High Resolution Melt ◽

E Coli ◽

Clostridioides Difficile ◽

Sensitivity Specificity

Over one hundred bacterial species have been determined to comprise the human microbiota in a healthy individual. Bacteria including Escherichia coli, Bacillus cereus, Clostridioides difficile, and Vibrio parahaemolyticus are found inside of the human body and B. cereus and E. coli are also found on the skin. These bacteria can act as human pathogens upon ingestion of contaminated food or water, if they enter an open wound, or antibiotics, and environment or stress can alter the microbiome. In this study, we present new polymerase chain reaction (PCR) high-resolution melt (HRM) assays to detect and identify the above microorganisms. Amplified DNA from C. difficile, E. coli, B. cereus, and V. parahaemolyticus melted at 80.37 ± 0.45 °C, 82.15 ± 0.37 °C, 84.43 ± 0.50 °C, and 86.74 ± 0.65 °C, respectively. A triplex PCR assay was developed to simultaneously detect and identify E. coli, B. cereus, and V. parahaemolyticus, and cultured microorganisms were successfully amplified, detected, and identified. The assays demonstrated sensitivity, specificity, reproducibility, and robustness in testing.

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Susceptibility of Escherichia coli and Clostridium perfringens to sucrose monoesters of capric and lauric acid

Czech Journal of Animal Science ◽

10.17221/7588-cjas ◽

2014 ◽

Vol 59 (No. 8) ◽

pp. 374-380

Author(s):

E. Skřivanová ◽

Š. Pražáková ◽

O. Benada ◽

P. Hovorková ◽

MarounekM

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Clostridium Perfringens ◽

Lauric Acid ◽

Cytoplasmic Membrane ◽

E Coli ◽

Enteropathogenic Bacteria ◽

Viable Cells ◽

Transmission Electron ◽

Cytoplasmic Structures

The sucrose monoesters of capric and lauric acid were tested for their antibacterial activity towards two foodborne enteropathogenic bacteria – Escherichia coli (CCM 3954 – serotype O6 and E22 – serotype O103) and Clostridium perfringens (CNCTC 5459 and CIP 105178). Antibacterial activity was evaluated by the plating technique. Sucrose monocaprate significantly decreased the number of viable cells of E. coli at all tested concentrations (0.1–5 mg/ml). The overnight incubation of C. perfringens with the sucrose ester of lauric acid at 0.1–5 mg/ml reduced the number of viable cells below the detection limit (2 log<sub>10</sub> CFU/ml). Incubating E. coli CCM 3954 and C. perfringens CNCTC 5459 with monoesters (0.1 and 2 mg/ml) did not influence the K<sup>+</sup> permeability of the cytoplasmic membrane in cells during a 2.5-minute treatment. A 30-minute incubation of E. coli CCM 3954 and C. perfringens CNCTC 5459 with esters (0.1 and 2 mg/ml) revealed damage to cytoplasmic structures, as observed by transmission electron microscopy.  

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Fate of Pathogens Inoculated onto Vacuum-Packaged Sliced Hams to Simulate Contamination During Packaging1

Journal of Food Protection ◽

10.4315/0362-028x-42.6.464 ◽

1979 ◽

Vol 42 (6) ◽

pp. 464-469 ◽

Cited By ~ 18

Author(s):

M. E. STILES ◽

L.-K. NG

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Salmonella Typhimurium ◽

Bacillus Cereus ◽

Clostridium Perfringens ◽

Subsequent Treatment ◽

Vacuum Packaging ◽

E Coli ◽

Survival And Growth ◽

And Staphylococcus Aureus

Ham and chopped ham from two manufacturers were contaminated with five enteropathogens: Bacillus cereus, Clostridium perfringens, Escherichia coli, Salmonella typhimurium and Staphylococcus aureus, at time of slicing and vacuum-packaging, to simulate contamination by manufacturer. Subsequent treatment of the samples, representing sound and undesirable retail handling and consumer use conditions, indicated marked differences in the fate of the pathogens between these products and within product type between the two manufacturers. Greatest differences were observed between the chopped ham products. All pathogens, except C. perfringens, grew actively in fresh ham and chopped ham with abusive holding at 30 and 21 C. After storage at 4 or 10 C for 30 days, B. cereus and C. perfringens were no longer detected, even after subsequent holding at 30 or 21 C for 24 h. E. coli survival and growth was variable, S. typhimurium survived well and grew under some conditions and S. aureus was generally inhibited at high levels of competition.

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