scholarly journals Elevated activity levels of activating autoantibodies to the GnRH receptor in patients with polycystic ovary syndrome

F&S Reports ◽  
2020 ◽  
Vol 1 (3) ◽  
pp. 299-304
Author(s):  
Elizabeth A. Weedin ◽  
Heather R. Burks ◽  
Xichun Yu ◽  
Hong Liang Li ◽  
Christopher E. Aston ◽  
...  
2021 ◽  
Author(s):  
Hongliang Li ◽  
Yankai Guo ◽  
Gege Zhang ◽  
Jielin Deng ◽  
Hayley Fischer ◽  
...  

2020 ◽  
Vol 4 (8) ◽  
Author(s):  
David C Kem ◽  
Hongliang Li ◽  
Xichun Yu ◽  
Elizabeth Weedin ◽  
Anna C Reynolds ◽  
...  

Abstract Objective Is polycystic ovary syndrome (PCOS) associated with activating autoantibodies (AAb) to the second extracellular loop (ECL2) of gonadotropin-releasing hormone receptor (GnRHR)? Design and Methods We retrospectively screened sera from 40 patients with PCOS and 14 normal controls (NCs) with regular menses using enzyme-linked immunosorbent assay (ELISA) for the presence of GnRHR-ECL2-AAb. We obtained similar data from 40 non-PCOS ovulatory but infertile patients as a control group (OIC) of interest. We analyzed GnRHR-ECL2-AAb activity in purified immunoglobulin (Ig)G using a cell-based GnRHR bioassay. Results The mean ELISA value in the PCOS group was markedly higher than the NC (P = .000036) and the OIC (P = .0028) groups. IgG from a sample of 5 PCOS subjects, in contrast to a sample of 5 OIC subjects, demonstrated a dose-dependent increase in GnRHR-stimulating activity qualitatively similar to the acute action of the natural ligand GnRH and the synthetic agonist leuprolide. The GnRHR antagonist cetrorelix significantly suppressed (P < .01) the elevated GnRHR activity induced by IgG from 7 PCOS patients while the IgG activity level from 7 OIC subjects was unchanged. Five other OIC subjects had relatively high ELISA values at or above the 95% confidence limits. On further study, 3 had normal or low activity while 2 had elevated IgG-induced GnRHR activity. One suppressed with cetrorelix while the other did not. The copresence of PCOS IgG increased the responsiveness to GnRH and shifted the dosage response curve to the left (P < .01). Conclusions GnRHR-ECL2-AAb are significantly elevated in patients with PCOS compared with NCs. Their presence raises important etiological, diagnostic, and therapeutic implications.


2016 ◽  
Vol 8 (10) ◽  
pp. 127 ◽  
Author(s):  
Farnaz Shishehgar ◽  
Fahimeh Ramezani Tehrani ◽  
Parvin Mirmiran ◽  
Sepideh Hajian ◽  
Ahmad Reza Baghestani ◽  
...  

<p>Polycystic Ovary Syndrome is the most common endocrinopathy in women of reproductive age. Although physical activity and weight loss has been proposed as the first line management in patients with PCOS, studies on physical activity in patients with PCOS are limited. The aims of this study were to compare levels of physical activity and its related factors between women with polycystic ovary syndrome and eumenorrheic non hirsute women. We compared the physical activity of our study group using the international physical activity questionnaire (IPAQ), using T-test or Mann-Whitney to compare the means of two groups. Step wise multivariate logistic regression was used to determine the relationship between physical activity and body mass index and demographic factors. The results showed that physical activity levels in PCOS women compared to controls were similar; however the time spent sitting in PCOS women was significantly higher than controls; (6.94±2.2 versus 6.09±2.63 hours, P&lt;0.001).</p><p>Low levels of physical activity were reported by 59.2% of cases and 57.1% of controls. A positive association between physical activity levels and BMI (P=0.001) and age (P=0.03) was observed in PCOS women. In controls, physical activity levels was found to be associated with BMI (P=0.01), education (P=0.03) and pregnancy status (P=0.03). Considering the results of this study, it was concluded that In PCOS women, the impacts of demographic factors on physical activity may be less important than controls.</p>


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