Determination of pentamidine transfer in the in vitro perfused human cotyledon with high-performance liquid chromatography

1989 ◽  
Vol 160 (3) ◽  
pp. 759-761 ◽  
Author(s):  
Stephen J. Fortunato ◽  
Roger E. Bawdon
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

scholarly journals High-performance Liquid Chromatography Analysis and Antimicrobial Activities of Libyan Cistus salviifolius Extract

2021 ◽  
pp. 32-40
Author(s):  
Abdullah Alamami ◽  
Fatma Elshibani ◽  
Salmin Alshalmani ◽  
Mohamed Ali Sharkasi ◽  
Naema Elremali ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Cistus Salviifolius

Aims: This research is focused on the in vitro evaluation of Cistus salviifolius L. antimicrobial properties and the determination of the contents of phenols and flavonoids. Study Design: This research is analytical study aimed to illustrate the antimicrobial properties and to analyze the methanolic extract contents of aerial parts of Cistus salviifolius L. by high-performance liquid chromatography techniques. Duration: The study was performed within six months in the Faculty of Pharmacy, Benghazy University. Methodology: Antimicrobial properties was tested against twelve organisms using Kirby-Bauer disk diffusion sensitivity test and the determination of the contents of phenols and flavonoids was evaluated by running high-performance liquid chromatography techniques. Results: The findings indicated that catechin is the most abundant flavonoid in C. salviifolius, while gallic acid was the major phenol in the methanolic extract of the plant. The results also revealed that the methanol extracts had a significant antimicrobial potential particularly against Bacillus subtilis and Escherichia coli with MIC (0.98 and 0.49) µg/ml respectively, furthermore the extract was effective against Aspergillus fumigatus with MIC 0.98 µg/ml.       Conclusion: C. salviifolius was highly rich with flavonoids and phenols and has a significant antimicrobial effect.

Sign in / Sign up

Export Citation Format

Share Document