T1259 Serum Metabolite Profiles Differentiate Crohn's Disease From Ulcerative Colitis and From Healthy Controls

2010 ◽  
Vol 138 (5) ◽  
pp. S-523 ◽  
Author(s):  
Colette Johnston ◽  
Warwick Dunn ◽  
David Broadhurst ◽  
Marie Brown ◽  
Royston Goodacre ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Healthy Controls ◽  
Metabolite Profiles

PTU-033 Serum metabolite profiles differentiate Crohn's disease from ulcerative colitis and from healthy controls

Gut ◽  
2010 ◽  
Vol 59 (Suppl 1) ◽  
pp. A61.2-A61
Author(s):  
C Johnston ◽  
W Dunn ◽  
D Broadhurst ◽  
M Brown ◽  
A Makin ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Healthy Controls ◽  
Metabolite Profiles

Μελέτη έκφρασης ορολογικών δεικτών σε ασθενείς με ΙΦΝΕ από την ΒΔ Ελλάδα και συσχέτιση με κλινικοεργαστηριακά δεδομένα και περιβαλλοντικούς παράγοντες

2019 ◽  
Author(s):  
Καλλιρρόη Κυριακίδη
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Research Laboratory ◽  
Scientific Data ◽  
Diagnosis And Treatment ◽  
Surgical Removal ◽  
Healthy Controls ◽  
Serological Markers ◽  
Number Of Patients

Ulcerative colitis and Crohn's disease belong to the same group of Idiopathic Inflammatory Bowel Disease (IBD) with unknown aetiopathogenesis. IBD is a chronic disease with outbursts and recessions. Although symptoms are usually treated with appropriate treatment, they impair the daily activities of the person causing significant morbidity. According to epidemiological studies that have been conducted both internationally and in our area, the number of patients with IBD is constantly increasing, suggesting the existence of an unknown environmental agent that goes into everyday life and causes disease in genetically predisposed individuals. In recent years, research into the aetiopathogenesis of IBD has increased sharply as the problem is continually increasing and therapeutically complicated. Diagnosis and treatment of IBD is very difficult, and despite the various diagnostic tests and available pharmaceutical preparations, some patients are forced to undergo surgical removal of the bowel and other types of disability. For the above reasons, patients are monitored in specialized reference centers by specialized scientists. FThe purpose of this study is to study serological markers, their expression and their utility in the prevention, diagnosis and treatment of patients with IBD. More specifically, some known serological markers with proven specificity / sensitivity were studied in patient with IBD. In the present study, 116 biological samples of serum from patients with Crohn's disease (NK) and 92 biological serum samples from patients with ulcerative colitis (UC) were studied and 99 healthy controls (CTRLS) were also studied. Patients were diagnosed with IBD based on clinical, endoscopic, radiological and pathological criteria and voluntarily participated in this research, which was conducted at the Immunology Research Laboratory of the Medical School of the University of Ioannina in collaboration with the Gastroenterological Clinic of the General Hospital of Ioannina. The samples came from populations in Northwest Greece. The study concerned the existence of ANCA antibodies by the immunofluorescence technique using a kit with ethanol, in particular the separation of pANCA and cANCA antibodies. We also studied the presence of ASCA antibodies using a kit using the ELISA technique. We studied all patients and healthy controls for the presence of ANCA and ASCA antibodies. The results of the study enriched one of the largest databases of patients with IBD, in addition the results of the serological markers were combined with other clinical data from the database by the same patients and healthy controls. This objective was achieved as the Immunology Research Laboratory is one of the largest centers of study and recording of IBD on a pan-Hellenic scale. The study, recording and processing of the data was done using appropriate research statistical methods and modern data analysis of scientific data.

scholarly journals Systemic Molecular Mediators of Inflammation Differentiate Between Crohn’s Disease and Ulcerative Colitis, Implicating Threshold Levels of IL-10 and Relative Ratios of Pro-inflammatory Cytokines in Therapy

2019 ◽  
Vol 14 (1) ◽  
pp. 118-129 ◽  
Author(s):  
Miranda G Kiernan ◽  
J Calvin Coffey ◽  
Shaheel M Sahebally ◽  
Paul Tibbitts ◽  
Emma M Lyons ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Inflammatory Cytokines ◽  
Healthy Controls ◽  
Mediators Of Inflammation ◽  
Faecal Diversion ◽  
Inflammatory Bowel ◽  
Circulating Fibrocytes ◽  
Pro Inflammatory Cytokines

Abstract Background and Aims Faecal diversion is associated with improvements in Crohn’s disease but not ulcerative colitis, indicating that differing mechanisms mediate the diseases. This study aimed to investigate levels of systemic mediators of inflammation, including fibrocytes and cytokines, [1] in patients with Crohn’s disease and ulcerative colitis preoperatively compared with healthy controls and [2] in patients with Crohn’s disease and ulcerative colitis prior to and following faecal diversion. Methods Blood samples were obtained from healthy individuals and patients with Crohn’s disease or ulcerative colitis. Levels of circulating fibrocytes were quantified using flow cytometric analysis and their potential relationship to risk factors of inflammatory bowel disease were determined. Levels of circulating cytokines involved in inflammation and fibrocyte recruitment and differentiation were investigated. Results Circulating fibrocytes were elevated in Crohn’s disease and ulcerative colitis patients when compared with healthy controls. Smoking, or a history of smoking, was associated with increases in circulating fibrocytes in Crohn’s disease, but not ulcerative colitis. Cytokines involved in fibrocyte recruitment were increased in Crohn’s disease patients, whereas patients with ulcerative colitis displayed increased levels of pro-inflammatory cytokines. Faecal diversion in Crohn’s disease patients resulted in decreased circulating fibrocytes, pro-inflammatory cytokines, and TGF-β1, and increased IL-10, whereas the inverse was observed in ulcerative colitis patients. Conclusions The clinical effect of faecal diversion in Crohn’s disease and ulcerative colitis may be explained by differing circulating fibrocyte and cytokine responses. Such differences aid in understanding the disease mechanisms and suggest a new therapeutic strategy for inflammatory bowel disease.

scholarly journals P147 Metabolomics discriminate children with Crohn's disease from ulcerative colitis and from healthy controls – preliminary study

2017 ◽  
Vol 11 (suppl_1) ◽  
pp. S150-S150
Author(s):  
U. Daniluk ◽  
M. Ciborowski ◽  
J. Daniluk ◽  
K. Pietrowska ◽  
A. Kretowski ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Healthy Controls ◽  
Preliminary Study

P194 Alexithymia traits are associated with symptom severity and disease activity in IBD patients

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S260-S260
Author(s):  
E Vinni ◽  
K Karaivazoglou ◽  
T Lourida ◽  
M Kalogeropoulou ◽  
E Tourkochristou ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Disease Activity ◽  
Symptom Severity ◽  
University Hospital ◽  
Control Group ◽  
Healthy Controls ◽  
Psychological Burden ◽  
Emotional Recognition

Abstract Background Alexithymic traits are quite prevalent in patients with inflammatory bowel diseases (IBD) and have been linked with a heightened psychological burden including anxiety, depression, somatization and obsessive-compulsive symptoms. However, there are limited data regarding the association of alexithymia with gastrointestinal symptom severity and disease activity. Methods The current study was conducted at the Division of Gastroenterology of the University Hospital of Patras, in Greece with the collaboration of the Department of Psychiatry. Adult IBD outpatients and healthy controls were enrolled to the study. Alexithymic traits were assessed with the Toronto Alexithymia Scale (TAS-20). Disease activity was assessed with the calculation of the Truelove-Witts Index for ulcerative colitis (UC) patients and the Harvey-Bradsaw Index for Crohn’s disease (CD) patients. Results 57 IBD patients and 50 healthy controls entered the study. 26 (45.6%) IBD patients reported clinically significant alexithymia traits. In addition, IBD patients faced increased difficulties in emotional expression (p=0.024) and reported increased overall alexithymic traits (p=0.033) compared to healthy controls. Moreover, we detected a borderline tendency of IBD patients to exhibit more disturbances in emotional recognition (p=0.067) compared to the control group. Difficulties in emotional recognition were significantly correlated with the presence of nausea (p=0.031), belly pain (p=0.015) and bloating (p=0.018), while overall alexithymic traits were strongly associated with the presence of nausea (p=0.008). Finally, increased disease activity was significantly associated with a higher total alexithymia score in Crohn’s disease (p=0.037) and ulcerative colitis (p=0.047) patients. Conclusion IBD patients report increased alexithymic traits which are associated with more severe gastrointestinal symptomatology and increased disease activity.

scholarly journals P018 Per- and polyfluoroalkyl substances (PFAS) are significantly increased in patients with late-onset of ulcerative colitis

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S138-S139
Author(s):  
F Fart ◽  
S Salihovic ◽  
A McGlinchey ◽  
M Orešič ◽  
J Halfvarson ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Bile Acids ◽  
Late Onset ◽  
Acid Synthesis ◽  
Healthy Controls ◽  
Triple Quadrupole Mass Spectrometer ◽  
Serum Samples ◽  
Polyfluoroalkyl Substances

Abstract Background Environmental factors have been implicated in the pathogenesis of inflammatory bowel disease (IBD), particularly late onset disease. Per- and polyfluoroalkyl substances (PFAS) are man-made chemicals with a long biological half-life that have been extensively used since the 1950s and have been proposed to interfere with the bile acid synthesis. Therefore, to investigate if late onset IBD correlates with higher PFAS levels, we measured serum levels of PFAS and bile acids in patients diagnosed with IBD later in life. Methods Serum samples were collected from patients diagnosed with ulcerative colitis (n = 20) and Crohn’s disease (n = 20) at the age of ≥55 years. Blood donors (n = 20) were used as healthy controls and were matched by gender and age. The levels of PFAS and bile acids were assessed by ultra performance liquid chromatography coupled to a triple quadrupole mass spectrometer. Results The total amount of PFAS was significantly higher in patients with ulcerative colitis compared with healthy controls (p = 0.021) or patients with Crohn’s disease (p = 0.015). No difference was found in total PFAS levels between Crohn’s disease patients and healthy controls (p = 0.841). Seven out of 30 bile acids correlated to the total PFAS level. Conclusion Our results demonstrate that PFAS levels are increased in patients with late-onset of ulcerative colitis compared with Crohn’s disease patients and healthy controls. This finding indicates that PFAS might represent an environmental risk factor for ulcerative colitis. However, additional studies assessing the functional consequences of increased PFAS in late-onset ulcerative colitis are required to confirm this hypothesis.

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