Changes in connective tissue components in ulcer tissue during the healing process of acetic acid ulcer in rats

Abstract Objectives Vanadium has been reported to possess relevant therapeutic properties such as anti-diabetic and anti-tumoral. This study aimed at determining the effects of vanadium on experimentally induced colitis in rats. Methods Forty-five male Wistar rats (103 ± 3.90 g, n=15) were used for this study and were divided into three groups. Group 1 (Untreated control) had nothing added to their drinking, while groups 2 and 3 received sodium metavanadate at a dose of 50 and 200 mg/L respectively in their drinking water for 10 weeks. Colitis was thereafter induced by intra colonic administration of 1.50 mL of 6% acetic acid. Animals were sacrificed on day 0 (pre-induction), three- and seven-days post induction. Blood samples were collected for haematological variables and the distal 8 cm of the colon was collected for macroscopic, histological and biochemical (malondialdehyde-MDA, superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase- GPx and nitrite concentration- NO) assessment. Results Low dose vanadium proved beneficial in ameliorating acetic acid-induced colitis by improving both histopathological and haematological changes. Gross observation showed a faster healing rate in vanadium treated groups (50 and 200 mg/L) compared with untreated control at day 3 (40 and 26.20 vs. 2.50%) and day 7 (80 and 66.70 vs. 42%) respectively. Vanadium also appears to exert its beneficial effects on acetic acid-induced colitis via up regulation of antioxidant enzymes (SOD, CAT, GPx) and NO while decreasing the over production of MDA. Conclusions Vanadium at small concentration functions as an essential trace element and may be able to promote healing process during ulcerative colitis.

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Ulcer Protective and Ulcer Healing Activities of Aqueous and Methanolic Extracts of Leaves of Rumex Bequaertii De Wild (Polygonaceae) in Rats

Journal of Biology and Life Science ◽

10.5296/jbls.v6i2.7044 ◽

2015 ◽

Vol 6 (2) ◽

pp. 61

Author(s):

Gilbert Ateufack ◽

Breuil R. Dongmo Feudjio ◽

William Nana Yousseu ◽

Albert D. Atsamo ◽

Albert Kamanyi

Keyword(s):

Acetic Acid ◽

Significant Variation ◽

Healing Process ◽

Negative Control ◽

Chronic Ulcer ◽

Male Rats ◽

Control Group ◽

Traditional Use ◽

Mucus Production ◽

Methanolic Extracts

The aqueous and methanolic extracts of Rumex bequaertii leaves were investigated for their ability to prevent and treated ulceration of the gastric mucosa in animal models.For this, adult male rats three to four months old and weighing on average 180 g were used for both tests. The extracts were tested orally at doses of 125, 250 and 500 mg/kg, on ulcerations experimentally induced by HCl/ethanol and pylorus ligation (acute ulcer) and acetic acid (chronic ulcer).These extracts showed protective and healing properties on ulcer induced models. A dose of 500 mg/kg of the two extracts inhibited ulceration induced by HCl/ethanol by 98.86 and 91.59 % respectively. With pyloric ligation, no significant variation of the volume of gastric juices, pH and gastric acidity was observed compared to the negative control rats. The aqueous extract alone at different doses presented a significant reduction of the ulceration surface resulting from chronic ulcer induced with acetic acid compared to the negative control group that received distilled water. For all three models of gastric ulcer induction used, the mass of mucus significantly increased in the groups that received the extracts. There was no significant variation in plasmatic nitric oxide (NO) concentrationand gastric supernatant of rats treated with both extracts compared to both control groups. These results reveal that the healing process of the extracts is not achieved through the NO pathway. This process could be due to increase in mucus production and thus supports its traditional use of the plant

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Effects of Pfaffia glomerata (Spreng) pedersen aqueous extract on healing acetic acid-induced ulcers

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132008000400005 ◽

2008 ◽

Vol 51 (4) ◽

pp. 479-483 ◽

Cited By ~ 1

Author(s):

Cristina Setim Freitas ◽

Cristiane Hatsuko Baggio ◽

Samanta Luiza Araújo ◽

Maria Consuelo Andrade Marques

Keyword(s):

Acetic Acid ◽

Gastric Ulcer ◽

Acute Toxicity ◽

Aqueous Extract ◽

Healing Process ◽

Intraperitoneal Administration ◽

Oral Route ◽

Chronic Gastric Ulcer ◽

Pfaffia Glomerata ◽

Tissue Healing

The present study was carried out to evaluate the acute toxicity and the effect of the aqueous extract of the roots from Pfaffia glomerata (Spreng) Pedersen (Amaranthaceae) (AEP) on the prevention of acetic acid-induced ulcer and on the healing process of previously induced ulcers. The acute toxicity was evaluated in Swiss mice after oral administration of a single dose and the chronic gastric ulcer was induced with local application of acetic acid. The results showed that the LD50 of the extract was 684.6 mg.kg-1 for the intraperitoneal administration and higher than 10 mg.kg-1by the oral route. The administration of the AEP did not prevent ulcers formation. However, the AEP increased of the healing process of previously induced ulcers. The results suggest that AEP chronically administered promote an increase of tissue healing, after the damage induced by acetic acid and the extract seemed to be destituted of toxic effects in the mice by the oral route.

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Healing Process of Experimental Esophageal Ulcers Induced by Acetic Acid in Rats

Scandinavian Journal of Gastroenterology ◽

10.3109/00365528909091112 ◽

1989 ◽

Vol 24 (sup162) ◽

pp. 6-10 ◽

Cited By ~ 9

Author(s):

H. Tsuji ◽

Y. Fuse ◽

K. Kawamoto ◽

H. Fujino ◽

T. Kodama

Keyword(s):

Acetic Acid ◽

Healing Process ◽

Esophageal Ulcers

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Changes in Synthetic Activity of Sulfated Mucosubstances in Healing Process of Acetic Acid Ulcer in Rats and Effect of Anti-Ulcer Agents

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)52306-x ◽

1984 ◽

Vol 34 (4) ◽

pp. 480-482

Author(s):

Toshio SUWA ◽

Kiyomi FUKUSHIMA ◽

Kazuaki KYOGOKU ◽

Yo MORI

Keyword(s):

Acetic Acid ◽

Healing Process ◽

Synthetic Activity

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Effect of Basic Fibroblast Growth Factor on Perforated Chinchilla Tympanic Membranes

Ear Nose & Throat Journal ◽

10.1177/014556139707600812 ◽

1997 ◽

Vol 76 (8) ◽

pp. 559-564 ◽

Cited By ~ 11

Author(s):

Norman R. Friedman ◽

Charles G. Wright ◽

Karen S. Pawlowski ◽

William L. Meyerhoff

Keyword(s):

Growth Factor ◽

Connective Tissue ◽

Tympanic Membrane ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Healing Process ◽

Fibroblast Growth ◽

Short Term ◽

Tympanic Membrane Perforations ◽

Perforated Tympanic Membrane

Basic fibroblast growth factor (bFGF) is a polypeptide mitogen which stimulates proliferation of epidermal and connective tissue cells. When applied to tympanic membrane perforations it has been reported to enhance healing and produce connective tissue hyperplasia. Previous work with animal models has shown that hyperplastic alterations of the tympanic membrane play an essential role in cholesteatoma development. This study was designed to further investigate the hyperplastic effects of bFGF and to determine if it might induce cholesteatoma formation during the healing process. Ten chinchillas received bilateral tympanic membrane perforations. In each animal, three doses of bFGF (400 nanograms per dose) were applied to the perforated tympanic membrane on one side; the opposite (control) ear received saline alone. The animals were terminated at either two or four weeks and studied histologically. Although the dosage and administration schedule used were consistent with previous studies utilizing other rodent species, there was little evidence that bFGF affected tympanic membrane healing in chinchillas. In both control and bFGF-treated ears, dense connective tissue occupied the lamina propria of the tympanic membrane, providing an effective barrier against ingrowth of skin toward the middle ear. No cholesteatomas developed in any animals included in the study. The results of this work indicate that the risk of cholesteatoma formation following administration of bFGF is minimal when it is applied short-term to acute perforations.

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ID: 1058 Effective bone formation through BMP-2-immobilized highly porous GBR membrane

Biomedical Research and Therapy ◽

10.15419/bmrat.v4is.332 ◽

2017 ◽

Vol 4 (S) ◽

pp. 139

Author(s):

Min Ji Kim ◽

Jin Hyun Park ◽

Ho Yong Kim ◽

June Ho Byun ◽

Jin Ho Lee ◽

...

Keyword(s):

Connective Tissue ◽

Bone Formation ◽

Bone Regeneration ◽

Bone Defect ◽

Active Sites ◽

Healing Process ◽

The Body ◽

Bioactive Molecules ◽

Bone Morphogenetic Protein 2 ◽

Gbr Membrane

Sound healing of large bone defects is critical challenges in most of clinical fields. In general healing process of bone regeneration, the rapid infiltration of connective tissue, whereas relatively slow bone regeneration in bone defect leads to the incomplete bone formation. To solve this drawback, guide bone regeneration (GBR) membrane which could prevent rapid infiltration of connective tissue into bone defect, thus GBR membrane is feasible for compact bone regeneration in clinical fields. In recent, the most researchers believed that bioactive molecules-grafted GBR membranes may enhance the bone regeneration. To allow graft of bioactive molecules from porous membrane, chemically modified scaffolds are commonly used. This modification leads to sufficient interaction with active sites of bioactive molecules to stable the immobilization of bioactive molecules in the body. However it is hard to apply to clinical applications because of the toxicity of chemical residue used for the modification. In this study, we developed a GBR membrane with leaf-stacked structure which can allow sustained release of bone morphogenetic protein-2 (BMP-2) without any additional modification. The morphology, mechanical property, BMP-2 release profile, osteogenic differentiation of human periosteum-derived cells, and new bone formation efficiency of the BMP-2-loaded GBR membrane compared with commercial product were investigated.

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