Determination of abamectin residues in fruits and vegetables by high-performance liquid chromatography

Three carbamate pesticides, aldicarb (A0), ethiofencarb (E0), methiocarb (M0) and six of their oxidized metabolites, sulphoxides (A1, E1, M1) and sulphones (A2, E2, M2) were simultaneously determined. Five grams of a sample were homogenized with acetone, and then treated with dichloromethane-hexane mixture (1:1) and sodium chloride (NaCl), homogenized and centrifuged. The organic layer was removed and the aqueous residue was reextracted a second time with dichloromethane - hexane mixture. The combined organic extracts were evaporated in vacuo. The residue was dissolved in dichloromethane and charged on a Sep-Pak® aminopropyl cartridge. Carbamates were eluted from the cartridge with 1% methanol in dichloromethane. The eluate was evaporated to dryness and 0.5 ml of methanol and 1.5 ml of 0.001 N-hydrochloric acid (HCl) solution were added. Individual carbamates were separated by gradient elution high performance liquid chromatography (HPLC) using octyldecylsaline (ODS) column. Derivatization of separated carbamates to fluorescent derivatives was achieved in-line. Recovery of pesticides and their oxidized metabolites from rice, apple, cabbage and other foods ranged from 60 to 103% following fortification at 20 ppb. Detection limits were 1 ppb for A1, A2, E1, E2, 2 ppb for A0, E0, M1 and 4 ppb for M0 and M2 (S/N>3).

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Rapid and Simple Method for Determination of Thiram in Fruits and Vegetables with High-Performance Liquid Chromatography with Ultraviolet Detection

Journal of Agricultural and Food Chemistry ◽

10.1021/jf980910o ◽

1998 ◽

Vol 46 (12) ◽

pp. 5302-5304 ◽

Cited By ~ 14

Author(s):

Susanne B. Ekroth ◽

Birgit Ohlin ◽

Bengt-Göran Österdahl

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Fruits And Vegetables ◽

Simple Method ◽

Ultraviolet Detection

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Evaluation of Some Pesticide Residues in Fruits import by High Performance Liquid Chromatography

Al-Mustansiriyah Journal of Science ◽

10.23851/mjs.v27i4.14 ◽

2017 ◽

Vol 27 (4) ◽

Author(s):

Lamees M. A. Salman ◽

Iyad Abdul-Muhsan Ahmed

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Pesticide Residues ◽

High Performance ◽

Fruits And Vegetables ◽

Current Knowledge ◽

Maximum Residue Limits ◽

Exposure Risks ◽

Core Mixture

Pesticide residues have been found in various fruits and vegetables. This study collected 24 samples and reported a method based on High Performance Liquid Chromatography (HPLC). For determination of pesticide residues used in Some fruits which were collected from different markets of Baghdad city to make) 24) samples from (peel , core, mixture) for each type of fruits markets as: Oranges( Egypt , Africa), Pomegranate ( Egypt), Mango ( Kenya ), Pears (China), Plum fruits ( Africa ) , Kiwi (Turkey ) .That detective of ( 5 ) different pesticides (diazinon, malathion, chlorpyrifos ,parathion and cypermethrin). The results were detected of multi-residues of pesticides on the fruit in ( peel , core , mixture ) may be in the limit of Maximum residue limits(MRL) or higher of it . The pesticides detected that exceeding the limits are: cypermethrin in Kiwi (peel, core and mixture)at(0.204,0.038, 0.537), in pomegranate detected in (peel and mixture) at (0.509,0.189) mg/kg , Diazinon detccted in Egyptian orange in (peel ,core and mixture) at (0.031,0.207, 0.099) mg/kg . Malathion and Parathion was not detected at any type of fruits and that results was compared with codex of FAO/WHO(2013). That data is important to monitor residues in food and to fill gaps in current knowledge would be helpful in assessing human exposure risks from ingestion of contaminated Fruits Imported to our country.

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