Ex vivo induction of cytokine mRNA expression in human blood samples

2001 ◽  
Vol 249 (1-2) ◽  
pp. 63-71 ◽  
Author(s):  
Christoph Härtel ◽  
Gregor Bein ◽  
Michael Müller-Steinhardt ◽  
Harald Klüter
Keyword(s):  
Mrna Expression ◽  
Human Blood ◽  
Ex Vivo ◽  
Cytokine Mrna ◽  
Blood Samples ◽  
Cytokine Mrna Expression ◽  
Human Blood Samples

scholarly journals Effects of Ginseng and Echinacea on Cytokine mRNA Expression in Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Deniz Uluışık ◽  
Ercan Keskin
Keyword(s):  
Immune System ◽  
Mrna Expression ◽  
Panax Ginseng ◽  
Echinacea Purpurea ◽  
Fischer 344 Rats ◽  
Cytokine Mrna ◽  
Blood Samples ◽  
Fischer 344 ◽  
Cytokine Mrna Expression ◽  
Ad Libitum

The aim of the study was to determine the effect of ginseng and echinacea on the mRNA expression of IL-10, TNF-α, and TGF-β1 in healthy rats. Six-week-old male Fischer 344 rats (n=48) were used. The animals were divided into three equal groups, as follows: control (C); ginseng (G); echinacea (E). While the C group was fed a standard rat diet (Purina)ad libitumfor a period of 40 days, the G and E groups animals received the same diet containing 0.5 g/kg ofPanax ginsengroot powder and 0.75 g/kg ofEchinacea purpurearoot powder, respectively. Blood samples were obtained from 8 rats in each group after 20 and 40 days of treatment, and the mRNA expression of IL-10, TNF-α, and TGF-β1 was determined. After 20 days of treatment, the expression of IL-10 mRNA in the G group was different from the C group (P<0.05); however, after 40 days of treatment, there was no difference between the groups. There was no difference after 20 and 40 days of treatment between the groups with respect to the expression of TGF-β1 mRNA. After 20 days of treatment, the expression of TNF-αmRNA in the E group was higher (P<0.05) than the C group. After 40 days of treatment, the expression of TNF-αmRNA was similar in all of the groups. Based on the current study, the increase in expression of IL-10 mRNA in the G group and the increase in expression of TNF-αmRNA in the E group support the use of these plants for purposes of modulating the immune system. However, a more detailed study regarding the effects of ginseng and echinacea on these cytokines and other cytokines is needed.

scholarly journals Characterization of Five Zoonotic Streptococcus suis Strains from Germany, Including One Isolate from a Recent Fatal Case of Streptococcal Toxic Shock-Like Syndrome in a Hunter

2015 ◽  
Vol 53 (12) ◽  
pp. 3912-3915 ◽  
Author(s):  
Tobias Eisenberg ◽  
Christoph Hudemann ◽  
Hamid M. Hossain ◽  
Angela Hewer ◽  
Khodr Tello ◽  
...  
Keyword(s):  
Human Blood ◽  
Ex Vivo ◽  
Fatal Case ◽  
Streptococcus Suis ◽  
Toxic Shock ◽  
Blood Samples ◽  
Content Type ◽  
Human Blood Samples ◽  
Low Levels

AStreptococcus suisisolate from a German hunter with streptococcal toxic shock-like syndrome (STSLS) and four additional zoonotic isolates were genotyped asmrp+epf* (variant 1890)sly+cps2+. All five zoonotic German strains were characterized by high multiplication in human blood samplesex vivo, but induction of only low levels of proinflammatory cytokines compared to a Chinese STSLS strain.

scholarly journals Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay

2021 ◽  
Author(s):  
Ezgi Eyluel Bankoglu ◽  
Franzisca Stipp ◽  
Johanna Gerber ◽  
Florian Seyfried ◽  
August Heidland ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Comet Assay ◽  
Human Blood ◽  
Ex Vivo ◽  
Human Biomonitoring ◽  
Dna Damage And Repair ◽  
Blood Samples ◽  
Repair Activity ◽  
Human Blood Samples

AbstractThe comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet assay in human biomonitoring became highly attractive due to its various modified versions, which may be useful to determine individual susceptibility in blood samples. However, in human biomonitoring studies, working with large sample numbers that are acquired over an extended time period requires some additional considerations. One of the most important issues is the storage of samples and its effect on the outcome of the comet assay. Another important question is the suitability of different blood preparations. In this study, we analysed the effect of cryopreservation on DNA damage and repair activity in human blood samples. In addition, we investigated the suitability of different blood preparations. The alkaline and FPG as well as two different types of repair comet assay and an in vitro hydrogen peroxide challenge were applied. Our results confirmed that cryopreserved blood preparations are suitable for investigating DNA damage in the alkaline and FPG comet assay in whole blood, buffy coat and PBMCs. Ex vivo hydrogen peroxide challenge yielded its optimal effect in isolated PBMCs. The utilised repair comet assay with either UVC or hydrogen peroxide-induced lesions and an aphidicolin block worked well in fresh PBMCs. Cryopreserved PBMCs could not be used immediately after thawing. However, a 16-h recovery with or without mitotic stimulation enabled the application of the repair comet assay, albeit only in a surviving cell fraction.

scholarly journals Delayed Cytokine mRNA Expression Kinetics after T-Lymphocyte Costimulation: A Quantitative Measure of the Efficacy of Cyclosporin A-based Immunosuppression

2002 ◽  
Vol 48 (12) ◽  
pp. 2225-2231 ◽  
Author(s):  
Christoph Härtel ◽  
Lutz Fricke ◽  
Nina Schumacher ◽  
Holger Kirchner ◽  
Michael Müller-Steinhardt
Keyword(s):  
T Cell ◽  
Mrna Expression ◽  
Kidney Transplant ◽  
Ex Vivo ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression ◽  
Tnf Α ◽  
Ex Vivo Study ◽  
Expression Kinetics

Abstract Background: Because cyclosporin A (CsA) and glucocorticoids inhibit the production of interleukin-2 (IL-2) and other cytokines, quantitative analysis of cytokine mRNA might constitute a pharmacodynamic measure for immunosuppressive drug effects. We investigated whether immunosuppressive drugs influence cytokine mRNA expression kinetics during T-cell costimulation. Methods: We used a human whole blood assay to determine basal (unstimulated) IL-2, IL-4, and tumor necrosis factor-α (TNF-α) mRNA concentrations and expression kinetics after anti-CD3/anti-CD28 monoclonal antibody costimulation in kidney transplant recipients undergoing CsA-based immunosuppressive triple therapy and in healthy controls (ex vivo study I). The effect of CsA on IL-2 mRNA expression kinetics was also determined ex vivo in patients undergoing CsA monotherapy (ex vivo study II) and after in vitro addition of CsA. Results: In ex vivo study I, basal TNF-α mRNA but not IL-2 and IL-4 mRNA was decreased in kidney transplant patients. We observed shifts in peak IL-2 and IL-4 (from 8 to 24 h) and TNF-α (from 4 to 8 h of costimulation) mRNA expression in kidney transplant patients after T-cell costimulation. In patients undergoing CsA monotherapy (ex vivo study II), the inhibitory effect of CsA was detectable as an individually delayed increase in IL-2 mRNA during costimulation. In vitro addition of CsA also induced a dose-independent displacement of IL-2 mRNA expression kinetics (i.e., a delay). Conclusions: A delayed increase in cytokine mRNA expression during T-cell costimulation may represent a sensitive effect of immunosuppression. The single analysis of one absolute or peak mRNA value could be misleading. For prospective studies involving measurement of cytokine mRNA, we therefore suggest the parameter “area of cytokine mRNA expression over time”, which should include absolute cytokine mRNA values at two different time points of mRNA kinetics.

scholarly journals A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16339-16350
Author(s):  
Mengkui Ding ◽  
Ling Zha ◽  
Hui Wang ◽  
Jinyao Liu ◽  
Peiwu Chen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Carcinoembryonic Antigen ◽  
Human Blood ◽  
Shell Structure ◽  
Core Shell ◽  
Label Free ◽  
Blood Samples ◽  
Human Blood Samples ◽  
Core Shell Structure ◽  
Electrochemical Immunosensing

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.

Borrelia burgdorferi Survival in Human Blood Samples

1988 ◽  
Vol 539 (1 Lyme Disease) ◽  
pp. 444-445 ◽  
Author(s):  
GUY BARANTON ◽  
ISABELLE SAINT-GIRONS
Keyword(s):  
Borrelia Burgdorferi ◽  
Human Blood ◽  
Blood Samples ◽  
Human Blood Samples
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