Evaluation of a high IgE-responder mouse model of allergy to bovine β-lactoglobulin (BLG): development of sandwich immunoassays for total and allergen-specific IgE, IgG1 and IgG2a in BLG-sensitized mice

2000 ◽  
Vol 235 (1-2) ◽  
pp. 21-32 ◽  
Author(s):  
Karine Adel-Patient ◽  
Christophe Créminon ◽  
Hervé Bernard ◽  
Gilles Clément ◽  
Luc Négroni ◽  
...  
Keyword(s):  
Mouse Model ◽  
Specific Ige ◽  
Β Lactoglobulin ◽  
Responder Mouse

scholarly journals Suppression of Airway Allergic Reactions by a Photocatalytic Filter Using Mouse Model

Toxics ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 40
Author(s):  
Taisuke Tomonaga ◽  
Hiroto Izumi ◽  
Chinatsu Nishida ◽  
Kaori Kato ◽  
Kazuhiro Yatera ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Mouse Model ◽  
Respiratory Tract ◽  
Bronchoalveolar Lavage ◽  
Experimental Model ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Specific Ige ◽  
Allergic Reactions ◽  
Living Organisms

Photocatalytic filters installed in air purifiers have been used to purify spaces by decomposing allergenic substances. However, we have not found any reports that evaluate the effectiveness of photocatalytic filters in suppressing allergic reactions in living organisms. In this study, we intratracheally instilled ovalbumin (OVA) into OVA-sensitized mice after the OVA was photocatalyzed by a titanium dioxide (TiO2) filter, and verified the experimental model for evaluating the allergy-suppressing effect of photocatalysts. Mice were sensitized to OVA (10 µg/mouse) four times, and were intratracheally instilled with OVA (10 µg/mouse) after photocatalysis three times. Non-sensitized animals were instilled with normal saline following the same exposure schedule. The mice were dissected 24 h after final exposure. The OVA after photocatalysis significantly decreased the number of eosinophils in bronchoalveolar lavage fluid, and the concentration of OVA-specific IgE and IgG1 in serum, which were elevated in untreated OVA. Moreover, our experimental model showed the suppression of allergic reactions in mice, along with the decomposition of OVA after photocatalysis using the photocatalytic filter. Taken together, our experimental model for evaluating allergic reactions in the respiratory tract suggested that the allergy-suppressing effect of the photocatalytic filter can be evaluated.

scholarly journals Characterization of a Lactococcus lactis Strain That Secretes a Major Epitope of Bovine Beta-Lactoglobulin and Evaluation of Its Immunogenicity in Mice

2003 ◽  
Vol 69 (11) ◽  
pp. 6620-6627 ◽  
Author(s):  
Jean-Marc Chatel ◽  
Sebastien Nouaille ◽  
Karine Adel-Patient ◽  
Yves Le Loir ◽  
Herman Boe ◽  
...  
Keyword(s):  
Immune Response ◽  
Fusion Protein ◽  
Lactococcus Lactis ◽  
Immunoglobulin E ◽  
Specific Ige ◽  
Specific Immune Response ◽  
Lactis Strain ◽  
Beta Lactoglobulin ◽  
Β Lactoglobulin

ABSTRACT Bovine β-lactoglobulin (Blg) is one of the major cow's milk allergens. Peptide 41-60 of Blg (Blg41-60) was described as a murine T-cell determinant and a murine, rat, and human immunoglobulin E (IgE) epitope. The aim of this study was the expression of Blg41-60 as a fusion protein in the food-grade bacterium Lactococcus lactis and the characterization of its immunogenicity in mice. We constructed a recombinant strain of L. lactis capable of inducible production and secretion of Blg41-60::Nuc, a fusion protein between Blg41-60 and the mature part of the staphylococcal nuclease (Nuc). The highest production yield of Blg41-60::Nuc (32.5 mg/liter) was reached 4 h after induction. At this time, up to 75% of Blg41-60::Nuc was secreted. When monoclonal antibodies specific for Blg41-60 were used, purified Blg41-60::Nuc and synthetic Blg41-60 exhibited very similar immunoreactivities. Subcutaneous coadministration of purified Blg41-60::Nuc and killed nonrecombinant L. lactis resulted in the induction of specific anti-Blg41-60 IgG2a and IgG1. The IgG1/IgG2a ratio and the lack of specific IgE suggest a Th1-type immune response, i.e., a nonallergic response. Similar administrations of the killed Blg41-60::Nuc-producing L. lactis strain did not elicit a specific immune response, whereas a transitory mucosal IgA-specific immune response was induced in mice after oral administration of the live Blg41-60::Nuc-producing L. lactis strain.

Allergy

PEDIATRICS ◽  
1991 ◽  
Vol 87 (6) ◽  
pp. 985-992
Keyword(s):  
Solid Phase ◽  
Milk Proteins ◽  
Subsequent Development ◽  
Specific Ige ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
Β Lactoglobulin

Pathophysiology MATERNAL IMMUNE STATUS AGAINST β-LACTOGLOBULIN AND COW'S MILK ALLERGY IN THE INFANT Casmir GJA, Duchateau J, Cuvelier P, Vis HL. Ann Allergy. 1989;63:517-519 Purpose of the Study The reported frequency of cow's milk allergy in children varies between 0.3% and 7.5% (population with earlier weaning has higher incidence). A thorough family history of atopy and cord IgE level at birth have been used to predict infants "at risk." Previous studies have shown that high cord IgE level (exceeding 1 IU/mL) was predictive of subsequent development of atopic disease. The purpose of this study was to follow-up the predictive relationship of maternal IgG-anti-BLG (IgG against β-lactoglobulin which is a major cow's milk antigen) and allergic manifestations in the infant. Study Population: Subject Section Mothers. All women were selected from the obstetric department of St Pierre Hospital in Brussels, Belgium. Infants. 69 infants were chosen who were born to these mothers by vaginal delivery and were fed cow's milk. Methods Clinical Evaluation. Every month during the first 6 months of life, infants were examined clinically for allergic symptoms. Gastrointestinal signs (diarrhea, vomiting, colic), atopic dermatitis, and respiratory problems (asthma, chronic cough, rhinitis) were recorded. Symptomatic children were treated with Alfare (Nestle) containing hydrolysates of cow's milk proteins with minimal allergenicity. Biologic Investigations. IgG-anti-BLG levels in mothers' sera were measured using solid-phase radioimmunoassay method. These levels were expressed in arbitrary units per milliliter. All children were screened for total IgE levels at 5 days of age. Specific IgE anti-cow's milk radioallergosorbent tests (RASTs) were performed in all patients at 1 month of age.

Oral treatment with β-lactoglobulin peptides prevents clinical symptoms in a mouse model for cow's milk allergy

2013 ◽  
Vol 24 (7) ◽  
pp. 656-664 ◽  
Author(s):  
Laura A. P. M. Meulenbroek ◽  
Betty C. A. M. van Esch ◽  
Gerard A. Hofman ◽  
Constance F. den Hartog Jager ◽  
Alma J. Nauta ◽  
...  
Keyword(s):  
Mouse Model ◽  
Clinical Symptoms ◽  
Oral Treatment ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
Β Lactoglobulin

Treatment with synthetic β-lactoglobulin peptides can prevent clinical symptoms in a mouse model for cow's milk allergy

2014 ◽  
Vol 2 (3) ◽  
pp. 84-85
Author(s):  
L.A.P.M. Meulenbroek ◽  
B.C.A.M. van Esch ◽  
G.A. Hofman ◽  
C.F. den Hartog Jager ◽  
A.J. Nauta ◽  
...  
Keyword(s):  
Mouse Model ◽  
Clinical Symptoms ◽  
Cow’S Milk Allergy ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Cow's Milk Allergy ◽  
Β Lactoglobulin

The effect of treatments on the allergenicity of ß-lactoglobulin in Moroccan population

2018 ◽  
Vol 48 (4) ◽  
pp. 538-548
Author(s):  
Ouarda Azdad ◽  
Najlae Mejrhit ◽  
Lotfi Aarab
Keyword(s):  
Design Methodology ◽  
Specific Ige ◽  
Cross Sectional Study ◽  
Cow Milk ◽  
Sectional Study ◽  
Cross Sectional ◽  
Content Type ◽  
Moroccan Population ◽  
Conformational Epitopes ◽  
Β Lactoglobulin

Purpose This study aims to evaluate the effect of heating and pepsin hydrolysis on the allergenicity of purified cow’s milk ß-lactoglobulin using individual sera from Moroccan population. Design/methodology/approach A cross-sectional study was carried out in Hospitals of Fez-Meknes region, to evaluate specific IgE to raw cow milk, as well as to heated and pepsin-hydrolyzed β-lactoglobulin. Findings Results showed that 6.6 per cent of studied patients presented high values of serial IgE. The effect of treatments on the allergenicity of ß-lactoglobulin showed that heating at 90°C and pepsin hydrolysis, for 1 h each, showed an important decrease in the recognition of human IgE with a maximum of reduction of 81 and 91 per cent, respectively. Originality/value This reduction of immunoreactivity of human IgE to treated ß-lactoglobulin suggested that this Moroccan population recognized mostly conformational epitopes.

3-Hydroxyphthaloyl β-Lactoglobulin. IV. Antiviral Activity in the Mouse Model of Genital Herpesvirus Infection

1998 ◽  
Vol 9 (4) ◽  
pp. 69-79 ◽  
Author(s):  
H Kokuba ◽  
L Aurelian ◽  
AR Neurath
Keyword(s):  
Antiviral Activity ◽  
Mouse Model ◽  
Major Protein ◽  
Viral Glycoprotein ◽  
Lesion Development ◽  
Simultaneous Exposure ◽  
Virus Shedding ◽  
Prophylactic Measures ◽  
Β Lactoglobulin ◽  
Hsv 1

The spread of sexually transmitted infections caused by herpes simplex virus type 2 (HSV-2) has continued unabated despite educational efforts generated in response to the human immunodeficiency virus (HIV) epidemic. Given the absence of effective vaccines, this indicates the need to develop prophylactic measures such as topical antiviral agents. Chemical modification of bovine β-lactoglobulin (β-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor designated 3HP-β-LG. This agent was shown to also have antiviral activity against HSV-2 and HSV-1 in vitro. Recent studies indicate that 3HP-β-LG binds to HSV-1 virions, which, at least in part, involves the viral glycoprotein gE. Here we show that 3HP-β-LG inhibits HSV-2 infection in the mouse model of genital HSV-2 infection. Simultaneous exposure to HSV-2 and 3HP-β-LG caused a significant decrease in the proportion of infected animals (27% virus shedding, 5% lesion development and 0% fatality for 3HP-β-LG as compared to 80% shedding, 60% lesion development and 53% fatality in micetreated with PBS). The proportion of animals with HSV-2 infection after treatment with β-LG was similar to that in the PBS-treated group. Pretreatment with 3HP-β-LG formulated in a gel, which prolongs the presence of the agent in the vagina, also significantly reduced the proportion of HSV-2-infected mice (5% virus shedding, 5% lesion development and 0% fatality for 3HP-β-LG as compared to 70% shedding, 60% lesion development and 40% fatality in vehicle-treated mice). These differences were significant ( P≤0.0005, 0.002 and 0.008 for shedding, lesion development and fatality, respectively). Virus titres in the minority of mice that developed infection were similar to those in untreated mice. HSV-2 infection was not inhibited by treatment of an ongoing infection, indicating that 3HP-β-LG interferes with the initial infection. These data suggest that 3HP-β-LG may be an efficacious agent for preventing vaginal transmission of genital herpesvirus infections.

scholarly journals Mouse Chow Composition Influences Immune Responses and Food Allergy Development in a Mouse Model

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1775 ◽  
Author(s):  
Eleonore Weidmann ◽  
Nazanin Samadi ◽  
Martina Klems ◽  
Denise Heiden ◽  
Klara Seppova ◽  
...  
Keyword(s):  
Food Allergy ◽  
Mouse Model ◽  
Immune Responses ◽  
Oral Tolerance ◽  
Specific Ige ◽  
Allergy Prevention ◽  
Lower Fatty Acid ◽  
Immunological Effect ◽  
Gastric Acid Suppression ◽  
Antibody Levels

Our diet is known to substantially influence the immune response not only by support of mucosal barriers but also via direct impact on immune cells. Thus, it was of great interest to compare the immunological effect of two mouse chows with substantial differences regarding micro-, macronutrient, lipid and vitamin content on the food allergic response in our previously established mouse model. As the two mouse chows of interest, we used a soy containing feed with lower fatty acid (FA) amount (soy-containing feed) and compared it to a soy free mouse chow (soy-free feed) in an established protocol of oral immunizations with Ovalbumin (OVA) under gastric acid suppression. In the animals receiving soy-containing feed, OVA-specific IgE, IgG1, IgG2a antibody levels were significantly elevated and food allergy was evidenced by a drop of body temperature after oral immunizations. In contrast, mice on soy-free diet had significantly higher levels of IL-10 and were protected from food allergy development. In conclusion, soy-containing feed was auxiliary during sensitizations, while soy-free feed supported oral tolerance development and food allergy prevention.

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