Deoxyribonucleic acid secondary structure in the region of the replication point

1966 ◽  
Vol 17 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Chev Kidson
Keyword(s):  
Secondary Structure ◽  
Deoxyribonucleic Acid ◽  
Replication Point

scholarly journals AMINOACRIDINE DYES AND THE SECONDARY STRUCTURE OF DNA IN SITU

1971 ◽  
Vol 19 (12) ◽  
pp. 761-765 ◽  
Author(s):  
MANUEL DIAZ ◽  
JOSE HIERRO ◽  
GRACIELA DEMICHELI DE DIAZ
Keyword(s):  
Secondary Structure ◽  
Acid Solution ◽  
Nitrous Acid ◽  
Deoxyribonucleic Acid ◽  
New Method ◽  
Green Fluorescence ◽  
Double Stranded Dna

A new method is proposed to study the secondary structure of deoxyribonucleic acid (DNA) in situ in fixed chromatin. It is based on acriflavine staining and on differentiation with a nitrous acid solution of the fixed cytologic preparation. The presence of green fluorescence after this treatment is regarded as indicative of double stranded DNA. Experiments are described with DNA-acriflavine mixtures in solution, DNA-agar models and cytologic preparations submitted to different pretreatments. The feasibility and limitations of the method are discussed in the light of the results reported upon.

scholarly journals The effects of deoxyribonucleic acid secondary structure on tertiary structure

1976 ◽  
Vol 159 (3) ◽  
pp. 615-620 ◽  
Author(s):  
A M Campbell
Keyword(s):  
Ionic Strength ◽  
Secondary Structure ◽  
Deoxyribonucleic Acid ◽  
Chromosome Structure ◽  
Tertiary Structure ◽  
Limiting Factors ◽  
Branch Points ◽  
Linear Dna ◽  
Dna Strands ◽  
Molecular Dimensions

The secondary structure of supercoiled DNA was varied by changes in ionic strength. For I = 0.075-0.4 the structure remained in the previously established branched form with only minor alterations in molecular dimensions. In 4M-NaCl, which induces linear DNA to change its secondary structure to the C structure and brings about an increase in the superhelix density of the molecule, no extra branches were observed on the molecules. The limiting factors that dictate supercoil structure seem to be the number and position of potential branch points and the proximity with which the two intertwining DNA strands can approach each other on the arms of the branches. This value is close to 10nm under the conditions described, and is 14-15nm at I = 0.2. It is suggested that such values should be borne in mind when models of chromosome structure are being constructed.

scholarly journals Deoxyribonucleic Acid Polymerase Associated with Avian Tumor Viruses: Secondary Structure of the Deoxyribonucleic Acid Product

1971 ◽  
Vol 7 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Lois Fanshier ◽  
Axel-Claude Garapin ◽  
Jerome McDonnell ◽  
Anthony Faras ◽  
Warren Levinson ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Deoxyribonucleic Acid ◽  
Acid Product ◽  
Tumor Viruses

THE ULTRAVIOLET SPECTRA OF RIBONUCLEIC ACIDS IN SOLID STATE AND IN SOLUTION

1965 ◽  
Vol 43 (12) ◽  
pp. 3151-3159 ◽  
Author(s):  
Michael Falk
Keyword(s):  
Aqueous Solution ◽  
Solid State ◽  
Secondary Structure ◽  
Relative Humidity ◽  
Ribonucleic Acid ◽  
Room Temperature ◽  
Deoxyribonucleic Acid ◽  
Ribonucleic Acids ◽  
Solid Films ◽  
Spectral Changes

The ultraviolet absorption spectra of nine samples of soluble, ribosomal, and viral ribonucleic acid (RNA) were studied over the range 3 500 to 1 830 Å. The spectra and the spectral changes which occur upon denaturation were the same for all types of RNA examined, which suggests that their secondary structure is comparable. Dehydration of solid films of RNA at room temperature caused spectral changes resembling those which occur when RNA is heated in aqueous solution. This indicates that the dehydration of RNA, like the dehydration of deoxyribonucleic acid (DNA), causes some loss of the regular stacking and pairing of the nucleotide bases in the helical regions. In contrast to DNA, the rehydration of RNA was not always complete at 98% relative humidity. The decrease of absorbance in the 2 900 Å region upon denaturation, previously reported by Rich and Kasha, was not confirmed with any of the RNA samples studied.

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

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