The effects of ascorbic acid and sodium azide on seedling growth of irradiated and non-irradiated barley seeds

Antimutagenicity of vitaminâ€C (ascorbic acid) and Lâ€cysteine (amino acid) was determined against the mutagenicity of sodium azide (NaN3; pH 6.0) in Trigonella foenumâ€graecum L. Toxicity to seed germination and seedling growth were taken as as the parameters. Sodium azide (mutagenic agent) induces inhibitory effects on the seed germination and causes formation of abnormal seedlings significantly with increasing concentrations. Sodium azide mutagenized seeds of T. foenumâ€graecum postâ€treated with four different aqueous concentrations of vitaminâ€C (0.0005M, 0.0010M, 0.0015M and 0.0100M) and Lâ€cysteine (0.0005M, 0.0010M, 0.0015M and 0.0100M), exhibited antimutagenic activity by way of promotory effects on seed germination as well as on seedling growth as compared to contol. Lâ€cystein was found to be more potent as antimutagen (antitoxic) than vitaminâ€C.

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Biochemical Basis of Heterosis in Sorghum: Changes in Chlorophylls and Ascorbic Acid Turnover during Seedling Growth*

Annals of Botany ◽

10.1093/oxfordjournals.aob.a086023 ◽

1981 ◽

Vol 47 (3) ◽

pp. 321-328 ◽

Cited By ~ 5

Author(s):

K. C. BHATT ◽

P. P. VAISHNAV ◽

Y. D. SINGH ◽

J. J. CHINOY

Keyword(s):

Ascorbic Acid ◽

Seedling Growth ◽

Biochemical Basis

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THE EFFECT OF ASCORBIC ACID OXIDATION ON THE INCORPORATION OF SULFATE BY SLICES OF CALF COSTAL CARTILAGE

The Journal of General Physiology ◽

10.1085/jgp.42.2.303 ◽

1958 ◽

Vol 42 (2) ◽

pp. 303-318 ◽

Cited By ~ 16

Author(s):

S. J. Klebanoff ◽

D. D. Dziewiatkowski ◽

G. J. Okinaka

Keyword(s):

Hydrogen Peroxide ◽

Ascorbic Acid ◽

Sodium Azide ◽

Costal Cartilage ◽

Dehydroascorbic Acid ◽

Inhibitory Effect ◽

Considerable Extent ◽

Acid Oxidation ◽

Marked Inhibition ◽

Production Of Hydrogen

A marked inhibition of the incorporation of S35-sulfate by normal calf costal cartilage was produced by potassium ascorbate in the presence of catalytic amounts of cupric ions. The effect of the various components of the ascorbic acid oxidizing system (potassium ascorbate, cupric ions, cuprous ions, hydrogen peroxide, dehydroascorbic acid) was investigated. The results of experiments in which hydrogen peroxide, catalase, or sodium azide were used singly or in combination suggest that the inhibition produced by the ascorbic acid oxidizing system is due, to a considerable extent, to the production of hydrogen peroxide. Dehydroascorbic acid was also found to inhibit the incorporation of S35-sulfate by cartilage slices. However, the gradual fall in pH which resulted from the addition of dehydroascorbic acid could account to a large extent for the inhibitory effect observed because the incorporation of S35-sulfate by cartilage slices decreases sharply as the pH is lowered. The incorporation of S35-sulfate by cartilage slices is inhibited also by increasing the concentration of phosphate.

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Effect of Different Colour Shading on Seedling Growth, Fruit Morphology and Phytochemical Properties of Tomato (Solanum lycopersicum L. var KWR)

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i530520 ◽

2020 ◽

pp. 72-84

Author(s):

P. K. Dissanayake ◽

W. G. C. Wekumbura

Keyword(s):

Ascorbic Acid ◽

Seed Germination ◽

Seedling Growth ◽

Acid Content ◽

Ascorbic Acid Content ◽

Consumer Preference ◽

Small Scale ◽

Fruit Morphology ◽

Tomato Plants ◽

Fruit Setting

Context: Producing high quality and chemical free tomato would favorably enhance consumer preference. There are most promising findings available on growth and quality attributes of tomato and other plants under different light spectrum in closed house conditions using different light sources. Installment of light emitting devices and running cost with high power consumption might not favorable for commercial cultivators and small scale growers. Aim: In present study we used colour transparent sheets such as colourless (control), red, blue, green and yellow as shading material to transmit respective spectrum of light on tomato plants to study the effect. Methodology: Tomato seed germination study was done under different colour shading in petri plates. Seedling and plant growth parameters and phytochemical properties of tomato plants grown under different transparent colour shadings were studied such as number of leaves, leaf area, weight of shoot and root, chlorophyll content, ascorbic acid content of fruits, lycopene contents of fruits, fruit physical parameters and flowering. Results: Seed germination was significantly high under yellow, colourless and red shading over blue and green. Seedling growth was significantly high under yellow shading. Fruit Ascorbic acid content was positively enhanced by yellow, green and colourless shading, whereas chlorophyll and lycopene content significantly high under green and red. Number of flower setting per plant was high under green shading (40±5.77), whereas lowest under yellow shading (22.33±6.38). Fruit setting significantly high under yellow shading (14±2) and lowest was red (9±1). Blue shading showed significantly high effect on fruit morphological traits such as pericarp thickness and fruit size. Conclusion: Yellow colour shading enhances seedling growth and fruit setting of tomato, while green having positive impact on fruit morphology. However, different characters of tomato were differently affected by different colour shading in greenhouse condition and for the better effect appropriate colour shading should be selected at particular stage of growth.

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The Effect of Zinc Oxide Nanoparticles (ZnO NPs) on Vigna mungo L. Seedling Growth and Antioxidant Activity

Nanoscience &Nanotechnology-Asia ◽

10.2174/2210681208666180820150647 ◽

2020 ◽

Vol 10 (2) ◽

pp. 117-122

Author(s):

Kantabathini Venkata Pavani ◽

Mallula Beulah ◽

Govinda Udayar Sai Poojitha

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Glutathione Reductase ◽

Seedling Growth ◽

Vigna Mungo ◽

Guaiacol Peroxidase ◽

Zno Nps ◽

Wide Range ◽

The Impact

Aim: The purpose of this study was to test the phytotoxicity effect of ZnONPs on Vigna mungo L. seedling growth and antioxidant activity. Methods: Vigna mungo L. Seeds were treated with to a wide range of ZnO NPs ranging 5 to25mg/100ml for 8hours. Vigna mungo seeds that were soaked in ZnO NPs solution were sown in pots (20 cm × 40 cm) filled with red soil and a layer of coco peat. The effect of ZnO NPs on morphological, biochemical and antioxidant activity in Vigna mungo L. plants was investigated after 15,30,45 and 60 days. Results: The impact of ZnO NPs on plant growth characteristics and biochemical changes in Vigna mungo L. plants was investigated after 15,30,45 and 60 days. The ZnONPs exposure significantly enhanced germination percentage by 111.3% but root length (75.25%), shoot length (89.81%), number of leaves (91.66%), length of leaves (76%), width of leaves (67.27%), fresh weight of plant (27.96%) and dry weight of plant (28.23%) decreased in the treated plants after 60 days exposure to 25mg/100ml compared to the untreated control. Interestingly, treated plants after 60 days exposure to 25mg/100ml increased significantly the chlorophyll (115.0%), reducing sugars (244.4%), total sugars (212.72%) protein (181.8%). Treatment to Vigna mungo L. seeds with ZnONPs has been found to induce the activities of antioxidant enzymes such as Guaiacol peroxidase, Glutathione Reductase, Catalase and increase in the ascorbic acid and hydrogen peroxide contents. TEM images revealed that the aggregated ZnO NPs to be deposited inside the seed. Conclusion: Vigna mungo seeds treated with different concentrations of ZnO NPs showed decreased root growth and increased germination index, shoot and leaf growth. There was a significant change in Glutathione reductase, Guaiacol peroxidase and Catalase activity and ascorbic acid and hydrogen peroxide of Vigna mungo exposed to ZnONPs. Aggregated nanoparticles penetration into the intracellular region of the seed was observed.A complete study on the toxic effects of ZnO NPs can help significantly in the safe disposal of ENPs for the reduction of adverse effects in both environmental and agricultural systems.

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Zinc and ascorbic acid treatment alleviates systemic inflammation and gastrointestinal and renal oxidative stress induced by sodium azide in rats

Beni-Suef University Journal of Basic and Applied Sciences ◽

10.1186/s43088-021-00108-9 ◽

2021 ◽

Vol 10 (1) ◽

Author(s):

Akinleye Stephen Akinrinde ◽

Joseph Fapuro ◽

Kehinde Olugboyega Soetan

Keyword(s):

Ascorbic Acid ◽

Systemic Inflammation ◽

Sodium Azide ◽

Serum Levels ◽

Protein Carbonyl ◽

Glutathione S Transferase ◽

Protein Levels ◽

Protein Thiols ◽

Group A ◽

Group B

Abstract Background Sodium azide (NaN3) is a chemical of rapidly increasing economic importance but with high toxic attributes. In this study, the effects of zinc (Zn) and ascorbic acid (AsA) supplementation on sodium azide (NaN3)-induced toxicity in the stomach, colon and kidneys were evaluated in Wistar rats. Twenty-eight rats were randomly allocated to four experimental groups as follows: group A (control) given distilled water only; group B (NaN3 only, 20 mg/kg); group C (NaN3 + zinc sulphate, ZnSO4 80 mg/kg); and group D (NaN3 + AsA 200 mg/kg). Results NaN3 was found to significantly (p < 0.05) induce increases in serum nitric oxide (NO), advanced oxidation protein products (AOPP), myeloperoxidase (MPO) and total protein levels, along with significant (p < 0.05) increase in gastric, colonic and renal malondialdehyde (MDA) and protein carbonyl (PCO) levels. In addition, NaN3 induced significant (p < 0.05) reduction in superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities in the colon and kidneys. Treatment with Zn or AsA caused significant (p < 0.05) reduction in serum levels of oxidative and inflammatory markers, as well as tissue PCO and MDA levels. Moreover, co-treatment with Zn or AsA significantly (p < 0.05) restored colonic and renal levels of antioxidant enzymes, reduced glutathione and protein thiols. Conclusions This study shows that Zn or AsA supplementation alleviated NaN3 toxicity by suppressing systemic inflammation and preventing oxidative damage in the stomach, colon and kidneys of rats.

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