The Effect of Zinc Oxide Nanoparticles (ZnO NPs) on Vigna mungo L. Seedling Growth and Antioxidant Activity

2020 ◽  
Vol 10 (2) ◽  
pp. 117-122
Author(s):  
Kantabathini Venkata Pavani ◽  
Mallula Beulah ◽  
Govinda Udayar Sai Poojitha
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Glutathione Reductase ◽  
Seedling Growth ◽  
Vigna Mungo ◽  
Guaiacol Peroxidase ◽  
Zno Nps ◽  
Wide Range ◽  
The Impact

Aim: The purpose of this study was to test the phytotoxicity effect of ZnONPs on Vigna mungo L. seedling growth and antioxidant activity. Methods: Vigna mungo L. Seeds were treated with to a wide range of ZnO NPs ranging 5 to25mg/100ml for 8hours. Vigna mungo seeds that were soaked in ZnO NPs solution were sown in pots (20 cm × 40 cm) filled with red soil and a layer of coco peat. The effect of ZnO NPs on morphological, biochemical and antioxidant activity in Vigna mungo L. plants was investigated after 15,30,45 and 60 days. Results: The impact of ZnO NPs on plant growth characteristics and biochemical changes in Vigna mungo L. plants was investigated after 15,30,45 and 60 days. The ZnONPs exposure significantly enhanced germination percentage by 111.3% but root length (75.25%), shoot length (89.81%), number of leaves (91.66%), length of leaves (76%), width of leaves (67.27%), fresh weight of plant (27.96%) and dry weight of plant (28.23%) decreased in the treated plants after 60 days exposure to 25mg/100ml compared to the untreated control. Interestingly, treated plants after 60 days exposure to 25mg/100ml increased significantly the chlorophyll (115.0%), reducing sugars (244.4%), total sugars (212.72%) protein (181.8%). Treatment to Vigna mungo L. seeds with ZnONPs has been found to induce the activities of antioxidant enzymes such as Guaiacol peroxidase, Glutathione Reductase, Catalase and increase in the ascorbic acid and hydrogen peroxide contents. TEM images revealed that the aggregated ZnO NPs to be deposited inside the seed. Conclusion: Vigna mungo seeds treated with different concentrations of ZnO NPs showed decreased root growth and increased germination index, shoot and leaf growth. There was a significant change in Glutathione reductase, Guaiacol peroxidase and Catalase activity and ascorbic acid and hydrogen peroxide of Vigna mungo exposed to ZnONPs. Aggregated nanoparticles penetration into the intracellular region of the seed was observed.A complete study on the toxic effects of ZnO NPs can help significantly in the safe disposal of ENPs for the reduction of adverse effects in both environmental and agricultural systems.

scholarly journals Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from Suaeda vermiculata for Antioxidant and Cytotoxic Activities

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1501 ◽  
Author(s):  
Hamdoon A. Mohammed ◽  
Mohsen S. Al-Omar ◽  
Mahmoud Zaki El-Readi ◽  
Ahmad H. Alhowail ◽  
Maha A. Aldubayan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Cellulose ◽  
Cytotoxic Effect ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Water Soluble ◽  
Pheophytin A ◽  
Wide Range ◽  
Mcf 7

Background: This study is designed to discover a method for delivering an efficient potent pheophytin a (pheo-a) into more absorbed and small polymeric ethyl cellulose (EC) microparticles. Methods: Silica gel and Sephadex LH-20 columns were used to isolate pheo-a from the chloroform extract of the edible plant, Suaeda vermiculata. Pheo-a was incorporated into EC microparticles using emulsion-solvent techniques. The antioxidant activity of pheo-a microparticles was confirmed by the level of superoxide radical (SOD), nitric oxide (NO), and reducing power (RP) methods. Meanwhile, the cytotoxic effect of the product was investigated on MCF-7 cells using MTT assay. Results: Pheo-a was isolated from S. vermiculata in a 12% concentration of the total chloroform extract. The structures were confirmed by NMR and IR spectroscopic analysis. The formulated microparticles were uniform, completely dispersed in the aqueous media, compatible as ingredients, and had a mean diameter of 139 ± 1.56 µm as measured by a particle size analyzer. Pheo-a demonstrated a valuable antioxidant activity when compared with ascorbic acid. The IC50 values of pheo-a microparticles were 200.5 and 137.7 µg/mL for SOD, and NO respectively. The reducing power of pheo-a microparticles was more potent than ascorbic acid and had a 4.2 µg/mL for IC50 value. Pheo-a microparticles did not show notable cytotoxicity on the MCF-7 cell line (IC50 = 35.9 µg/mL) compared with doxorubicin (IC50 = 3.2 µg/mL). Conclusions: the results showed that water-soluble pheo-a microparticles were prepared with a valuable antioxidant activity in a wide range of concentrations with a noteworthy cytotoxic effect.

scholarly journals Amaranth Leaf Extract Protects Against Hydrogen Peroxide Induced Oxidative Stress in Drosophila Melanogaster

2021 ◽  
Author(s):  
Johnmark Ndinawe ◽  
Hellen W. Kinyi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Drosophila Melanogaster ◽  
Leaf Extract ◽  
Dependent Manner ◽  
Polyphenol Compounds ◽  
Dose Dependent

Abstract ObjectiveAmaranths leaves are rich in ascorbic acid and polyphenol compounds which have antioxidant activity. The aim of this study was to evaluate their in vivo antioxidant activity. The effect of consumption of Amaranth leaf extract on in vivo antioxidant activity, catalase enzyme activity and H2O2 induced oxidative stress in Drosophila melanogaster flies was assessed.ResultsConsumption of Amaranth leaf extract was associated with increased survival on exposure to H202 in a dose dependent manner in Drosophila melanogaster flies.

scholarly journals Development of a novel and rapid phenotype-based screening method to assess rice seedling growth

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Lena Vlaminck ◽  
Chananchida Sang-Aram ◽  
Deborah Botterman ◽  
Christine Jewel C. Uy ◽  
Mary Kay Harper ◽  
...  
Keyword(s):  
Plant Growth ◽  
Seedling Growth ◽  
Growth Regulation ◽  
Marine Invertebrates ◽  
Screening Method ◽  
Rice Seedling ◽  
Plant Growth Promoting Bacteria ◽  
Stress Tests ◽  
Wide Range ◽  
The Impact

Abstract Background Rice (Oryza sativa) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for Arabidopsis thaliana. Here, we present a phenotype-based screening method to study shoot-related parameters of rice seedlings via an automated computer analysis. Results The phenotype-based screening method was validated by testing several compounds in pharmacological experiments that interfered with hormone homeostasis, confirming that the assay was consistent with regard to the anticipated plant growth regulation and revealing the robustness of the set-up in terms of reproducibility. Moreover, abiotic stress tests using NaCl and DCMU, an electron transport blocker during the light dependent reactions of photosynthesis, confirmed the validity of the new method for a wide range of applications. Next, this method was used to screen the impact of semi-purified fractions of marine invertebrates on the initial stages of rice seedling growth. Certain fractions clearly stimulated growth, whereas others inhibited it, especially in the root, illustrating the possible applications of this novel, robust, and fast phenotype-based screening method for rice. Conclusions The validated phenotype-based and cost-efficient screening method allows a quick and proper analysis of shoot growth and requires only small volumes of compounds and media. As a result, this method could potentially be used for a whole range of applications, ranging from discovery of novel biostimulants, plant growth regulators, and plant growth-promoting bacteria to analysis of CRISPR knockouts, molecular plant breeding, genome-wide association, and phytotoxicity studies. The assay system described here can contribute to a better understanding of plant development in general.

scholarly journals Capsaicinoids, Polyphenols and Antioxidant Activities of Capsicum annuum: Comparative Study of the Effect of Ripening Stage and Cooking Methods

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 364 ◽  
Author(s):  
Mansor Hamed ◽  
Diganta Kalita ◽  
Michael E. Bartolo ◽  
Sastry S. Jayanty
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Capsicum Annuum ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Mature Stage ◽  
Total Phenolic ◽  
Cooking Methods ◽  
Wide Range ◽  
Green Stage

Peppers (Capsicum annuum L.) are an important crop usually consumed as food or spices. Peppers contain a wide range of phytochemicals, such as capsaicinoids, phenolics, ascorbic acid, and carotenoids. Capsaicinoids impart the characteristic pungent taste. The study analyzed capsaicinoids and other bioactive compounds in different pepper cultivars at both the mature green and red stages. The effect of roasting on their nutritional content was also investigated. In the cultivars tested, the levels of capsaicin ranged from 0 to 3636 µg/g in the mature green stage and from 0 to 4820 µg/g in the red/yellow stage. The concentration of dihydrocapsaicin ranged from 0 to 2148 µg/g in the mature green stage and from 0 to 2162 µg/g in the red/yellow stage. The levels of capsaicinoid compounds in mature green and red /yellow stages were either reduced or increased after roasting depending on the cultivar. The ranges of total phenolic and total flavonoids compounds were 2096 to 7689, and 204 to 962 µg/g, respectively, in the green and red/yellow mature stage pods. Ascorbic acid levels in the peppers ranged from 223 to 1025 mg/ 100 g Dry Weight (DW). Both raw and roasted peppers possessed strong antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl) reagent (DPPH, 61–87%) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS, 73–159 µg/g) assays. Ascorbic acid and antioxidant activity decreased after roasting in the mature green and red stages, whereas total phenolics and flavonoids increased except in the mature green stage of Sweet Delilah and yellow stage of Canrio.

Oxidative stress and antioxidant enzymes in triticale shoots under chloride salinization

2020 ◽  
Vol 63 (7) ◽  
pp. 99-105
Author(s):  
Viktor V. Ivanishchev ◽  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Cluster Analysis ◽  
Antioxidant Enzymes ◽  
Glutathione Reductase ◽  
Guaiacol Peroxidase ◽  
Short Term ◽  
Stress Indicators ◽  
Oxidative Stress Indicators

We studied alterations in oxidative stress indicators (hydrogen peroxide, superoxide radical, lipid peroxidation – LPO) and alterations in the activity of antioxidant enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase) in triticale shoots (Triticosecale) during short-term (0-96 h) sodium chloride stress (120 mM) with statistical methods: principal component analysis (PCA) and cluster analysis. Analysis of alterations in the activity of enzymes with the PCA method does not allow them to be unambiguously included in a single group, despite the fact that they all belong to antioxidant enzymes. The inclusion of oxidative stress indicators in this analysis did not make the picture simpler. Using the cluster analysis method, it can be concluded that under conditions of short-term chloride stress in the shoots of triticale, much more catalase (than other enzymes studied) is associated with the protection of membranes from lipid peroxidation than with the utilization of hydrogen peroxide. This is also reflected by the highest correlation coefficients: catalase – LPO (0.94), catalase – hydrogen peroxide (0.79). The formation of primary clusters between ascorbate peroxidase and glutathione reductase reflect the significance of the association of the ascorbate – glutathione cycle with the processes of utilization of reactive oxygen species (primarily hydrogen peroxide) under experimental conditions. It was also shown that under conditions of short-term chloride stress in the shoots of triticale, guaiacol peroxidase plays the least role in the utilization of hydrogen peroxide. In this case, salt ions again form a single primary cluster, which combines with other clusters at the maximum Euclidean distance in the experiment.

scholarly journals Meloidogyne javanica parasitism on the vegetative growth and nutritional quality of carrots

2020 ◽  
Vol 50 (9) ◽  
Author(s):  
Paula Juliana Grotto Débia ◽  
Beatriz Cervejeira Bolanho Barros ◽  
Heriksen Higashi Puerari ◽  
Claudia Regina Dias-Arieira
Keyword(s):  
Antioxidant Activity ◽  
Nutritional Quality ◽  
Meloidogyne Javanica ◽  
Proximate Analysis ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Negative Effects ◽  
Wide Range ◽  
The Impact

ABSTRACT: Meloidogyne javanica is a plant-parasitic nematode that infects a wide range of vegetables. Its negative effects on crop yield and value are well documented. However, few studies have investigated the impact of the parasite on the nutritional value of vegetables. This study aimed to assess the effect of M. javanica parasitism on the vegetative characteristics, nematological parameters, chemistry composition and antioxidant activity of carrots. Seedlings were inoculated with 0 (control), 1000, 2500, or 5000 eggs and eventual second-stage juveniles (J2) of M. javanica. At 60 days after inoculation, plants were harvested and evaluated. Plants inoculated with 2500 eggs and J2 of M. javanica had higher root and tuber fresh weight than the control. Gall number increased with increasing inoculum density. The number of nematodes in the roots increased until 3000 specimens, decreasing thereafter. Proximate analysis revealed that plants inoculated with 1000 eggs and J2 of M. javanica or more had higher protein content in roots. In contrast, inoculation with 1775 nematodes or more resulted in a decrease in carotenoid content. There was no effect of inoculation on total phenolic content or antioxidant activity. Although, M. javanica infection did not have a marked impact on the nutritional quality of carrots, gall formation resulted in deformed roots of low commercial value.

scholarly journals Antibacterial and Antioxidant activity of Benincasa hispida using Hydrogen peroxide scavenging model

2014 ◽  
Vol 2 (01) ◽  
pp. 86-94
Author(s):  
Rajesh Kumar Sharma ◽  
Rajni Singh ◽  
K. K. Jha ◽  
Abhishek B.
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Chloroform Extract ◽  
Acetone Extract ◽  
New Delhi ◽  
Standard Drug ◽  
Benincasa Hispida ◽  
Central Council ◽  
Antibacterial And Antioxidant Activity

The seeds of Benincasa hispida commonly known as ash gourd belonging to Cucurbitaceae family is employed as a main ingredient in kusmanda lehyam in Ayurvedic system of medicine. The seeds of Benincasa hispida mashed with milk or the various preparations from the pulp of fruit in the form of sweetmeats, like Kusmanda paka and petha are commonly used as a general tonic, aphrodisiac, rejuvenative and also a brain tonic. The plant of Benincasa hispida was collected from (Central Council for Research in Ayurveda and Siddha, Dept. of ayush, Ministry of Health and F.W, Govt. of India New Delhi) Govt. Central Pharmacy Annex, Ashoka Pillar, Jayanagar Bangalore. The extracts were subjected to Antioxidant activity by In hydrogen peroxide-scavenging model, model acetone extract showed scavenging (80.1%) of hydrogen peroxide and chloroform extract showed ( 79%) in comparison with ascorbic acid ( 94.5% ). Antioxidant activity was carried out for extract (acetone, chloroform, and aqueous extracts) with Hydrogen peroxide-scavenging model. Acetonic extract showed more scavenging (80.1%) of Benincasa hispida and least scavenging was done by aqueous extract of (43%). After comparison with the standard drug (Ascorbic acid) showed (94.5%). From this it was conclused that acetone extract of Benincasa hispida showed high scavenging activity. Conclusively, the result revealed that Benincasa hispida seeds has antimicrobial activity and antioxidant activity which may be due to the presence of alkaloids, phenolic compounds, flavonoids constituents present in the sample.

Study on Antioxidant Activity of Pigment in Black Soybean Peel

2014 ◽  
Vol 881-883 ◽  
pp. 378-383
Author(s):  
Qiong Jie Li ◽  
Chun Ling Xiao
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Anion ◽  
Raw Materials ◽  
Reducing Power ◽  
Liquid Ratio ◽  
Black Soybean ◽  
Solid Liquid ◽  
Better Than

With black soybean as raw materials, 50% ethanol as the extractant, at 70°C, pH1.0, solid-liquid ratio of 1:100(g:mL), at 90min were used to extract the pigment of black soybean Through the establishment of DPPH•,•OH, superoxide anion free radicals, reducing power and hydrogen peroxide. The results show: its ability of scavenging DPPH•, •OH, hydrogen peroxide is far better than ascorbic acid. The pigment at concentration of 0.6mg/mL shows a scavenging rate for DPPH• up to 88.29%,and for •OH 89.36%,for hydrogen peroxide89.75%.In addition，the general reducing power and scavenging rate for superoxide anion solution of the pigment are a little worse than those of ascorbic acid.

scholarly journals Indicators of the antioxidant system and oxidative stress of triticale shoots under chloride salinity

2020 ◽  
Vol 63 (9) ◽  
pp. 51-58
Author(s):  
Viktor V. Ivanishchev ◽  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Molecular Weight ◽  
Cluster Analysis ◽  
Glutathione Reductase ◽  
Ascorbate Peroxidase ◽  
Correlation Coefficients ◽  
Low Molecular Weight ◽  
Guaiacol Peroxidase ◽  
Short Term

Alterations in the activity of antioxidant enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase), the content of low molecular weight antioxidants (ascorbic acid, glutathione, and proline), as well as indicators of oxidative stress (hydrogen peroxide, superoxide radical, lipid peroxidation –LPO) in the shoots of triticale (Triticosecale) under short-term (0-96 h) sodium chloride stress (120 mM) were studied with statistical methods: principal component analysis (PCA) and cluster analysis. The application of the PCA method did not always lead to definite results that could be unambiguously interpreted from the point of view of modern concepts in the field of plant physiology and biochemistry. At the same time, high values of the correlation coefficients between individual indicators did not at all reflect their biochemical interdependence. The results obtained allow us to conclude that, under conditions of short-term chloride stress in triticale shoots, the more important function of proline is associated, rather, with the maintenance of osmotic pressure inside the cell than with the role of a low-molecular antioxidant. The mutual positive values of the correlation coefficients (with respect to each other) of the activity of catalase, glutathione reductase, LPO, the content of hydrogen peroxide and superoxide indicate the unidirectionality of the detected alterations, which reflect their known biochemical relationship and the need for glutathione reductase to maintain a certain redox-state in plant cells. With cluster analysis it was shown that under conditions of short-term chloride stress in triticale shoots, the studied enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase, and glutathione reductase) play a more significant role in neutralizing of reactive oxygen species, and maintaining the state of membranes, than low molecular weight antioxidants.

scholarly journals EVALUATION OF ANTIOXIDANT ACTIVITY OF CHLOROPHYLL

2019 ◽  
Vol 8 (6) ◽  
Author(s):  
Dipti Acharya ◽  
Umesh Kumar Gilhotra ◽  
Ranjan Singh ◽  
Himanshu Rajpurohit ◽  
Ajay Gar
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Methanolic Solution ◽  
Ic50 Value

Antioxidant play an important role to protect damage caused oxidative stress (OS).Chlorophyll having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties of methanolic solution from Super Chlorophyll. Objective: The main objective of the study was to evaluate the antioxidant activity of the chlorophyll in different validated in vitro models. Methods: The antioxidant activities of methanolic solution were evaluated by in vitro standard method using UV-Spectrophotometer. The antioxidant activity were determined bt total antioxidant capacity, DPPH (1-1-diphennyl-2-picrylhydrzine) radical scavenging assay, Nitric Oxide scavenging assay and Hydrogen Peroxide scavenging assay methods. Result: The solution of Chlorophyll was studied for antioxidant potential. Ascorbic acid was used as standard. This method is simple and activity of the solution is reported in term of IC50 value. Antioxidant properties depend on the IC50 value. Lesser the IC50 value, more is the antioxidant activity. Chlorophyll showed good antioxidant activity. Conclusion:  In case of in vitro antioxidant activity, Chlorophyll scavenged DPPH, hydrogen peroxide, nitric oxide radicals significantly, showed IC50 values near to standard ascorbic acid, thus proving to have good antioxidant potential. Keywords: Oxidative stress, DPPH, Antioxidant, Super chlorophyll.

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