Increased interleukin-13 (IL-13) concentrations in nasopharyngeal secretion in children with allergic asthma

2003 ◽  
Vol 111 (2) ◽  
pp. S301
Author(s):  
W. Feleszko ◽  
A. Zawadzka-Krajewska ◽  
K. Matysiak ◽  
D. Lewandowska ◽  
M. Kulus
Keyword(s):  
Allergic Asthma ◽  
Interleukin 13 ◽  
Nasopharyngeal Secretion

Preclinical Safety and Pharmacology of an Anti-Human Interleukin-13 Monoclonal Antibody in Normal Macaques and in Macaques with Allergic Asthma

2008 ◽  
Vol 27 (5) ◽  
pp. 351-358 ◽  
Author(s):  
Pauline L. Martin ◽  
Dusti Fisher ◽  
William Glass ◽  
Karyn O’Neil ◽  
Anuk Das ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Allergic Asthma ◽  
Immunoglobulin E ◽  
Cynomolgus Macaque ◽  
Lavage Fluid ◽  
Antigen Challenge ◽  
Interleukin 13 ◽  
Lung Eosinophilia

Interleukin-13 (IL-13) plays a central role in chronic airway diseases, including asthma. These studies were conducted to evaluate the safety of administration of a human anti-IL-13 monoclonal antibody (mAb) to normal macaques and in macaques with allergic asthma. In addition, serum and bronchioalveolar lavage fluid were collected from allergic cynomolgus macaques in order to identify potential surrogate markers of IL-13 pharmacology that could be useful for subsequent clinical trials. In vitro studies demonstrated that the anti-IL-13 mAb inhibited the pharmacological actions of both human and cynomolgus macaque IL-13. Allergic macaques were treated systemically with 10 mg/kg anti-IL-13 mAb 1 day prior to inhaled Ascaris suum antigen challenge. Normal macaques were dosed intravenously with anti-IL-13 once per week for 3 weeks at doses of 10 or 50 mg/kg. Treatment of macaques with the anti-IL-13 mAb was not associated with any toxicologically significant findings. A slight treatment-related but nonadverse decrease in platelet counts was observed in both the normal and allergic macaques. In allergic macaques, the anti-IL-13 mAb treatment did not affect lung function, lung eosinophilia, or serum or BAL immunoglobulin E (IgE) concentrations but did produce a reduction in BAL and serum eotaxin concentrations ( p < .05) at 6 h post antigen challenge. This study shows that administration of an anti-IL-13 mAb was well tolerated in both normal and allergic asthmatic macaques and that serum eotaxin concentrations may be a useful early in vivo marker for evaluating IL-13 inhibition in patients with asthma.

Interleukin-13: Central Mediator of Allergic Asthma

Science ◽  
1998 ◽  
Vol 282 (5397) ◽  
pp. 2258-2261 ◽  
Author(s):  
M. Wills-Karp ◽  
J. Luyimbazi ◽  
X. Xu ◽  
B. Schofield ◽  
T. Y. Neben ◽  
...  
Keyword(s):  
Allergic Asthma ◽  
Interleukin 13

scholarly journals Interleukin-13, Interleukin-10, Interferon-gamma and IDO Production in Response to Home Dust Mites in Allergic Asthma

2019 ◽  
Vol 11 (2) ◽  
pp. 194-9
Author(s):  
Cityta Putri Kwarta ◽  
Heri Wibowo ◽  
Yordan Khaedir ◽  
Iris Rengganis ◽  
Hanny Siti Nuraeni
Keyword(s):  
Allergic Asthma ◽  
Interleukin 10 ◽  
House Dust Mites ◽  
Atopic Asthma ◽  
Assay Method ◽  
Dust Mites ◽  
Interleukin 13 ◽  
Ifn Γ ◽  
Cellular Immune

BACKGROUND: Allergic asthma is a degenerative atopic disease caused by allergic or hypersensitivity type-1. More than 50% of people with allergic asthma are caused by the presence of house dust mites (HDMs) allergens.METHODS: The cellular immunity response was evaluated through a peripheral blood mononuclear cell (PBMC) culture isolated from blood, using the ficoll gradient technique. Subjects were atopic asthma groups and non-atopic asthma groups. PBMC from each subject cultured was stimulated with HDMs allergen, then incubated in a CO2 5% incubator, 37o C for 72 hours. With the multiplex assay method, interferon (IFN)-γ, interleukin (IL)-13 and IL-10 were measured, meanwhile indoleamine 2,3-dioxygenase level (IDO) was measured by the enzyme-linked immunosorbent assay (ELISA) sandwich methods.RESULTS: The IFN-γ production in the supernatant of PBMC cultures was stimulated by phytohemagglutinin (PHA), Roswell Park Memorial Institute (RPMI) medium and allergens. The IFN-γ production in allergen-stimulated supernatants showed higher level of IFN-γ in the nonatopic group (4,681,455±3,434,851) than atopic group (4,363,300±2,067,941) even though it was not statistically significant (p=0.078). There were no differences between the mean of IL-13 production in atopic asthma group and non-atopic group. The IL-10 production in allergenstimulated supernatants was shown to be higher in nonatopic group and were statistically significantly different (p=0.015). The IDO production in allergen-stimulated supernatants was shown to be higher in the non-atopic group (272,231±269,564) than in the actopic group (13,273±400), and it was significantly different (p=0.007).CONCLUSION: Cellular immune profile of subjects with allergic asthma to Dermatophagoides pterronyssinus (Der p) is characterized by a type-2 inflammatory response that is dominant compared to type-1 inflammation (higher IL-13 ratio compared to IFN-γ) and to the role of anti-inflammation (higher IL-13 ratio compared to IL-10). The decline in IDO production in allergic asthma subjects to Der p is thought to be related to the low cellular immune response in expressing IFN-γ compared to IL-13.KEYWORDS: interleukin-13, interleukin-10, IDO, PBMC, asthma

MODELING THE AIRWAY EPITHELIUM IN ALLERGIC ASTHMA: INTERLEUKIN-13– INDUCED EFFECTS IN DIFFERENTIATED MURINE TRACHEAL EPITHELIAL CELLS

2005 ◽  
Vol 41 (7) ◽  
pp. 217 ◽  
Author(s):  
SUSAN M. LANKFORD ◽  
MARIANGELA MACCHIONE ◽  
ANNE L. CREWS ◽  
SHAUN A. MCKANE ◽  
NANCY J. AKLEY ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Allergic Asthma ◽  
Airway Epithelium ◽  
Interleukin 13 ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

scholarly journals Serum Levels of Total IgE and Interleukin-13 in a Sample of Allergic Asthma Patients in Baghdad

2020 ◽  
pp. 3208-3214
Author(s):  
Mohammed Saleh Jebur ◽  
Asmaa Mohammed Saud
Keyword(s):  
Immune Response ◽  
Allergic Asthma ◽  
Immunosorbent Assay ◽  
Immunoglobulin E ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Interleukin 13 ◽  
Diagnosis And Management ◽  
Asthma Patients

Allergic asthma is a type of asthma that provokes symptoms when an individual is exposed to certain triggers, such as pollen, animal sources of allergens, and other various types of allergens. These allergens cause an immune response that influences lungs and leads to difficulties in breathing.  The current study is performed to estimate the concentrations of immunoglobulin E (IgE) and interleukin-13 (IL-13), tested by using the enzyme-linked immunosorbent assay (ELISA) and the numbers of eosinophils, calculated by using hematological analyzers, in the blood of patients with allergic asthma.  A total of 150 patients and 50 healthy individuals were randomly selected for the study. The results revealed that IgE and IL-13 levels as well as eosinophil percentage were significantly increased (p<0.001) in the patients in comparison to the healthy individuals. These parameters deem to be a key element in allergic asthma pathogenesis. They also help in the diagnosis and management of the disease.

Sequential degranulation of eosinophils induced by antigen or autocoids in allergic humans

1986 ◽  
Vol 44 ◽  
pp. 354-355
Author(s):  
Kate W. Sjoerdsma ◽  
W. James Metzger
Keyword(s):  
Allergic Rhinitis ◽  
Bronchoalveolar Lavage ◽  
Allergic Asthma ◽  
Skin Test ◽  
Positive Skin Test ◽  
Positive Skin ◽  
Human Eosinophil ◽  
Asthmatic Patients ◽  
Allergic Asthmatic ◽  
History Of

Eosinophils are important to the pathogenesis of allergic asthma, and are increased in bronchoalveolar lavage within four hours after bronchoprovocation of allergic asthmatic patients, and remain significantly increased up to 24 hours later. While the components of human eosinophil granules have been recently isolated and purified, the mechanisms of degranulation have yet to be elucidated.We obtained blood from two volunteers who had a history of allergic rhinitis and asthma and a positive skin test (5x5mm wheal) to Alternaria and Ragweed. Eosinophils were obtained using a modification of the method described by Roberts and Gallin.

Sign in / Sign up

Export Citation Format

Share Document