scholarly journals Serum Levels of Total IgE and Interleukin-13 in a Sample of Allergic Asthma Patients in Baghdad

2020 ◽  
pp. 3208-3214
Author(s):  
Mohammed Saleh Jebur ◽  
Asmaa Mohammed Saud
Keyword(s):  
Immune Response ◽  
Allergic Asthma ◽  
Immunosorbent Assay ◽  
Immunoglobulin E ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Interleukin 13 ◽  
Diagnosis And Management ◽  
Asthma Patients

Allergic asthma is a type of asthma that provokes symptoms when an individual is exposed to certain triggers, such as pollen, animal sources of allergens, and other various types of allergens. These allergens cause an immune response that influences lungs and leads to difficulties in breathing.  The current study is performed to estimate the concentrations of immunoglobulin E (IgE) and interleukin-13 (IL-13), tested by using the enzyme-linked immunosorbent assay (ELISA) and the numbers of eosinophils, calculated by using hematological analyzers, in the blood of patients with allergic asthma.  A total of 150 patients and 50 healthy individuals were randomly selected for the study. The results revealed that IgE and IL-13 levels as well as eosinophil percentage were significantly increased (p<0.001) in the patients in comparison to the healthy individuals. These parameters deem to be a key element in allergic asthma pathogenesis. They also help in the diagnosis and management of the disease.

scholarly journals Analysis ofLeishmaniamimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis

Parasitology ◽  
2018 ◽  
Vol 145 (14) ◽  
pp. 1938-1948 ◽  
Author(s):  
Lígia Moraes Barizon de Souza ◽  
Vanete Thomaz Soccol ◽  
Ricardo Rasmussen Petterle ◽  
Michelle D. Bates ◽  
Paul A. Bates
Keyword(s):  
Cell Surface ◽  
Cutaneous Leishmaniasis ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Cell Surfaces ◽  
Cell Markers ◽  
Antibody Titres

AbstractOligosaccharides are broadly present onLeishmaniacell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβis the immunodominant saccharide inLeishmaniacell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P< 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.

scholarly journals HUBUNGAN KADAR VITAMIN D SERUM DENGAN KUALITAS HIDUP PADA PASIEN EPILEPSI

2018 ◽  
Vol 35 (4) ◽  
Author(s):  
Edith Fitriyana Girsang ◽  
Aris Catur Bintoro ◽  
Dwi Pudjonarko
Keyword(s):  
Quality Of Life ◽  
Vitamin D ◽  
Immunosorbent Assay ◽  
Serum Vitamin ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epilepsy Patient ◽  
Vitamin D Levels ◽  
Spearman Test

  THE CORRELATION BETWEEN VITAMIN D SERUM LEVELS WITH QUALITY OF LIFE IN EPILEPSY PATIENTABSTRACTIntroduction: Epilepsy affects overall health status and decreases the life quality of epilepsy patient. Seizure frequency, seizure type, daily activity disorder, depression and anxiety also affect the quality of life epilepsy patient. Vitamin D is considered as a neurosteroid modulator of nerve excitability and seizure susceptibility. Studies of vitamin D direct role in epilepsy are limited. Nevertheless, some studies show the role of vitamin D as an anticonvulsant that reduces the incidence of seizures.Aim: To determine the correlation between vitamin D serum levels with quality of life in epilepsy patient.Method: A cross sectional observational study on people with epilepsy in neurology clinic at Dr. Soeselo Hospital, Slawi, Dr Kariadi Hospital, and Tugurejo Hospital, Semarang in July 2017-January 2018. The quality of life was assessed with Quality of Life in Epilepsy 31 (QOLIE-31). Vitamin D serum levels measured using enzyme-linked immunosorbent assay (ELISA) method. Data were analyzed using Spearman test. Results were considered significant if p<0.05.Results: There was no correlation between vitamin D serum levels with quality of life in epilepsy. There was significant correlation between anxiety with quality of life and there were difference between age group with quality of life. Discussion: There was no correlation between serum vitamin D levels and  quality of life in epilepsy patient.Keywords: Epilepsy,quality of life epilepsy, vitamin DABSTRAKPendahuluan: Epilepsi mempengaruhi status kesehatan secara keseluruhan dan menurunkan kualitas hidup pasien epilepsi. Frekuensi bangkitan, tipe bangkitan, gangguan aktivitas harian, depresi dan ansietas, juga memengaruhi kualitas hidup pasien epilepsi. Vitamin D dianggap neurosteroid, sebagai modulator eksitabilitas saraf dan kerentanan bangkitan. Bukti langsung untuk peran vitamin D dalam epilepsi terbatas. Namun beberapa penelitian menunjukkan peran vitamin D sebagai antikonvulsan yang mengurangi kejadian bangkitan.Tujuan: Mengetahui hubungan kadar vitamin D serum dengan kualitas hidup pada pasien epilepsi.Metode: Penelitian potong lintang terhadap pasien epilepsi yang berobat ke Poliklinik Saraf RSUD Dr. Soeselo, Slawi, RSUP Dr. Kariadi, Semarang, dan RS Tugurejo, Semarang pada bulan Juli 2017-Januari 2018. Kualitas hidup dinilai menggunakan kuesioner Quality of Life in Epilepsy 31 (QOLIE-31), pengukuran kadar vitamin D serum menggunakan metode enzyme-linked immunosorbent assay (ELISA). Data dianalisis dengan uji Spearman, hasil dianggap bermakna jika p<0,05.Hasil: Tidak didapatkan hubungan antara kadar vitamin D serum dengan kualitas hidup serta domainnya pada pasien epilepsi. Didapatkan hubungan yang bermakna antara ansietas dengan kualitas hidup dan antara kelompok usia dengan kualitas hidup.Diskusi: Tidak terdapat hubungan bermakna antara kadar vitamin D serum dengan kualitas hidup pada pasien epilepsi.Kata kunci: Epilepsi, kualitas hidup, vitamin D

Preclinical Safety and Pharmacology of an Anti-Human Interleukin-13 Monoclonal Antibody in Normal Macaques and in Macaques with Allergic Asthma

2008 ◽  
Vol 27 (5) ◽  
pp. 351-358 ◽  
Author(s):  
Pauline L. Martin ◽  
Dusti Fisher ◽  
William Glass ◽  
Karyn O’Neil ◽  
Anuk Das ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Allergic Asthma ◽  
Immunoglobulin E ◽  
Cynomolgus Macaque ◽  
Lavage Fluid ◽  
Antigen Challenge ◽  
Interleukin 13 ◽  
Lung Eosinophilia

Interleukin-13 (IL-13) plays a central role in chronic airway diseases, including asthma. These studies were conducted to evaluate the safety of administration of a human anti-IL-13 monoclonal antibody (mAb) to normal macaques and in macaques with allergic asthma. In addition, serum and bronchioalveolar lavage fluid were collected from allergic cynomolgus macaques in order to identify potential surrogate markers of IL-13 pharmacology that could be useful for subsequent clinical trials. In vitro studies demonstrated that the anti-IL-13 mAb inhibited the pharmacological actions of both human and cynomolgus macaque IL-13. Allergic macaques were treated systemically with 10 mg/kg anti-IL-13 mAb 1 day prior to inhaled Ascaris suum antigen challenge. Normal macaques were dosed intravenously with anti-IL-13 once per week for 3 weeks at doses of 10 or 50 mg/kg. Treatment of macaques with the anti-IL-13 mAb was not associated with any toxicologically significant findings. A slight treatment-related but nonadverse decrease in platelet counts was observed in both the normal and allergic macaques. In allergic macaques, the anti-IL-13 mAb treatment did not affect lung function, lung eosinophilia, or serum or BAL immunoglobulin E (IgE) concentrations but did produce a reduction in BAL and serum eotaxin concentrations ( p < .05) at 6 h post antigen challenge. This study shows that administration of an anti-IL-13 mAb was well tolerated in both normal and allergic asthmatic macaques and that serum eotaxin concentrations may be a useful early in vivo marker for evaluating IL-13 inhibition in patients with asthma.

scholarly journals TGF-Β as a Marker of Ulcerative Colitis and Disease Severity

2018 ◽  
Vol 19 (3) ◽  
pp. 229-236 ◽  
Author(s):  
Marina Jovanovic ◽  
Natasa Zdravkovic ◽  
Ivan Jovanovic ◽  
Gordana Radosavljevic ◽  
Nevena Gajovic ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Previous Treatment ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Marker ◽  
Compensatory Mechanism ◽  
Healthy Individuals ◽  
Highly Sensitive ◽  
Inflammatory Processes ◽  
Ifn Γ

Abstract Ulcerative colitis (UC) represents chronic inflammation of the large intestine. Immune response plays an important role in disease genesis and progression. Activated leukocytes secrete several cytokines that actively regulate the inflammatory response in UC. The aim of this study was to determine levels of cytokines IL-17, IL-27, IFN-γ and TGF-β in patients with UC and to test them as biomarkers for disease. The blood samples of 24 patients with ulcerative colitis without previous treatment and 37 healthy individuals were analyzed. Serum levels of IL-17, IL-27, IFN-γ and TGF-β were measured using sensitive enzyme-linked immunosorbent assay (ELISA) kits. Serum levels of IL-17, IL-27, IFN-γ and TGF-β were increased in patients with UC, compared to healthy controls (p=0.022; p=0.001; p=0.001; and p=0.002; respectively). Ratios of cytokines IL-27/IL-17, IFN-γ/TGF-β and IL-17/TGF-β were significantly higher in group of patients with UC (p=0.002; p=0.002; p=0.003; respectively). Serum value of TGF-β higher than 20 pg/ml presents a highly sensitive and specific marker for UC. We believe that increased production and predominance of immunosupressive TGF-β may represent compensatory mechanism for ongoing pro-inflammatory processes in UC.

scholarly journals Bovine Immunoglobulin E Levels of Bali Cattlesin Bangli and Nusa Penida island Bali Province, Indonesia

2019 ◽  
Vol 2 (2) ◽  
pp. 64
Author(s):  
Ni Ketut Suwiti ◽  
Luh Gde Surya Heryani ◽  
Desak Nyoman Dewi Indira Laksmi ◽  
Ni Nyoman Werdi Susari ◽  
I Nengah Kerta Besung ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Commercial Enzyme ◽  
Elisa Kit ◽  
Bali Cattle ◽  
Bovine Immunoglobulin

The aim of this research was to detect identify levels of Bovine Immunoglobulin E (BoIg.E), can be used as an indicator of response immune in bali cattle.  Eighty serum samples were collected from Nusa Penida and Bangli region. Bovine Ig.E levels was measured using a commercial Enzyme Linked Immunosorbent Assay (ELISA) Kit. The data were analysis based on differences of farming characteristics andgeographic. The result of research that, of BoIg.E level of bali cattle kept in Bangli (34.16258 ?g/ml), was higher than Nusa Penida (22.26047 ?g/ml). We conclude that there was a significant effect of differences of farming characteristics and geographic conditions.

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