Chitosan IFN-gamma-gene nanoparticle (CIN) therapy for allergic asthma in mice involves STAT4 signaling pathway

2003 ◽  
Vol 111 (2) ◽  
pp. S354
Author(s):  
X. Kong ◽  
M. Kumar ◽  
A. Behera ◽  
G. Hellerman ◽  
R.F. Lockey ◽  
...  
Keyword(s):  
Allergic Asthma ◽  
Signaling Pathway ◽  
Ifn Gamma

scholarly journals Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258051
Author(s):  
Zhijuan Xie ◽  
Haidan Sun ◽  
Xiaogang Li ◽  
Wei Sun ◽  
Jia Yin
Keyword(s):  
Allergic Asthma ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Birch Pollen ◽  
Control Group ◽  
Tandem Mass ◽  
Allergen Specific Immunotherapy ◽  
Before And After ◽  
Leukocyte Extravasation ◽  
Asthma Group

Subcutaneous immunotherapy (SCIT) is a classic form of allergen-specific immunotherapy that is used to treat birch pollen induced allergic asthma. To investigate the underlying molecular mechanisms of SCIT, we aimed to profile lung samples to explore changes in the differential proteome before and after SCIT in mice with allergic asthma. Fresh lungs were collected from three groups of female BALB/c mice: 1) control mice, 2) birch pollen-induced allergic mice, and 3) birch pollen-induced allergic mice with SCIT. Tandem mass tag (TMT) labelling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze the lung proteome in the mice. Ingenuity pathway analysis (IPA) and Gene Ontology (GO) classification analysis were applied to identify differentially expressed proteins (DEPs) and crucial pathways. The screened DEPs were validated by immunohistochemistry analysis. A total of 317 proteins were upregulated and 184 proteins were downregulated in the asthma group compared to those of the control group. In contrast, 639 DEPs (163 upregulated and 456 downregulated proteins) were identified after SCIT in comparison with those of the asthma group. Among the 639 DEPs, 277 proteins returned to similar levels as those of the relative non-asthma condition. Bioinformatic analysis revealed that the 277 proteins played a significant role in the leukocyte extravasation signaling pathway. The leukocyte extravasation signaling pathway and related DEPs were of crucial importance in birch pollen SCIT.

Cross-talk of Signaling Pathways in the Pathogenesis of Allergic Asthma and Cataract

2020 ◽  
Vol 27 (9) ◽  
pp. 810-822
Author(s):  
Yang Zhao ◽  
Sumei Liu ◽  
Xiangsheng Li ◽  
Zhenzhen Xu ◽  
Lifang Hao ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Allergic Asthma ◽  
Signaling Pathway ◽  
Academic Research ◽  
Notch Signaling Pathway ◽  
Mapk Signaling ◽  
Chronic Inflammatory Disease ◽  
Smad Signaling Pathway ◽  
Immune Imbalance ◽  
Cellular Components

: Allergic asthma is a chronic inflammatory disease, which involves many cellular and cellular components. Cataract is a condition that affects the transparency of the lens, which the opacity of the lens caused by any innate or acquired factor degrades its transparency or changes in color. Both of them belong to diseases induced by immune disorders or inflammation. We want to confirm the signaling pathways involved in the regulation of asthma and cataract simultaneously, and provide reference for the later related experiments. So we conducted a scoping review of many databases and searched for studies (Academic research published in Wiley, Springer and Bentham from 2000 to 2019) about the possible relationship between asthma and cataract. It was found that during the onset of asthma and cataract, Rho/Rock signaling pathway, Notch signaling pathway, Wnt/β-catenin signaling pathway, PI3K/AKT signaling pathway, JAK/STAT signaling pathway, MAPK signaling pathway, TGF-β1/Smad signaling pathway and NF-κB signaling pathway are all active, so they may have a certain correlation in pathogenesis. Asthma may be associated with cataract through the eight signaling pathways, causing inflammation or immune imbalance based on allergy that can lead to cataract. According to these studies, we speculated that the three most likely signaling pathways are PI3K/AKT, MAPK and NF-κB signaling pathway.

scholarly journals Activation of the STAT signaling pathway can cause expression of caspase 1 and apoptosis.

1997 ◽  
Vol 17 (9) ◽  
pp. 5328-5337 ◽  
Author(s):  
Y E Chin ◽  
M Kitagawa ◽  
K Kuida ◽  
R A Flavell ◽  
X Y Fu
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Signaling Pathway ◽  
Hela Cells ◽  
Similar Response ◽  
Dependent Manner ◽  
Ifn Gamma ◽  
Caspase 1 ◽  
Stat Signaling ◽  
Induced Apoptosis

Protein tyrosine kinases activate the STAT (signal transducer and activator of transcription) signaling pathway, which can play essential roles in cell differentiation, cell cycle control, and development. However, the potential role of the STAT signaling pathway in the induction of apoptosis remains unexplored. Here we show that gamma interferon (IFN-gamma) activated STAT1 and induced apoptosis in both A431 and HeLa cells, whereas epidermal growth factor (EGF) activated STAT proteins and induced apoptosis in A431 but not in HeLa cells. EGF receptor autophosphorylation and mitogen-activated protein kinase activation in response to EGF were similar in both cell lines. The breast cancer cell line MDA-MB-468 exhibited a similar response to A431 cells, i.e., STAT activation and apoptosis correlatively resulted from EGF or IFN-gamma treatment. In addition, in a mutant A431 cell line in which STAT activation was abolished, no apoptosis was induced by either EGF or IFN-gamma. We further demonstrated that both EGF and IFN-gamma induced caspase 1 (interleukin-1beta converting enzyme [ICE]) gene expression in a STAT-dependent manner. IFN-gamma was unable to induce ICE gene expression and apoptosis in either JAK1-deficient HeLa cells (E2A4) or STAT1-deficient cells (U3A). However, ICE gene expression and apoptosis were induced by IFN-gamma in U3A cells into which STAT1 had been reintroduced. Moreover, both EGF-induced apoptosis and IFN-gamma-induced apoptosis were effectively blocked by Z-Val-Ala-Asp-fluoromethylketone (ZVAD) in all the cells tested, and studies from ICE-deficient cells indicated that ICE gene expression was necessary for IFN-gamma-induced apoptosis. We conclude that activation of the STAT signaling pathway can induce apoptosis through the induction of ICE gene expression.

scholarly journals Schisandrin B Attenuates Airway Inflammation by Regulating the NF-κB/Nrf2 Signaling Pathway in Mouse Models of Asthma

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yaqin Chen ◽  
Yu Kong ◽  
Qili Wang ◽  
Jian Chen ◽  
Hua Chen ◽  
...  
Keyword(s):  
Allergic Asthma ◽  
Signaling Pathway ◽  
Airway Hyperresponsiveness ◽  
Inflammatory Responses ◽  
Related Factor ◽  
Inflammatory Disorder ◽  
Schisandrin B ◽  
Traditional Chinese Herbal Medicine ◽  
Nrf2 Signaling Pathway ◽  
Anti Inflammatory

Background. Asthma is a complex inflammatory disorder that plagues a large number of people. Schisandrin B is an active ingredient of the traditional Chinese herbal medicine Schisandra with various proven physiological activities such as anti-inflammatory and antioxidant activities. In this study, we explored the anti-inflammatory and antioxidant effects and provided the mechanistic insights into the activity of schisandrin B in a mouse model of ovalbumin- (OVA-) induced allergic asthma. Methods. Male BALB/c mice were sensitized and challenged with OVA to induce asthma and treated with various doses (15 mg/kg, 30 mg/kg, and 60 mg/kg) of SCH to alleviate the features of allergic asthma, airway hyperresponsiveness, inflammatory response, OVA-specific immunoglobulin (Ig)E level, and pathological injury. Results. Schisandrin B significantly attenuated the airway hyperresponsiveness induced by OVA. Moreover, schisandrin B administration suppressed inflammatory responses, reduced the level of IgE, and attenuated pathological injury. Mechanistically, schisandrin B treatment promoted the activation of nuclear erythroid 2-related factor 2 (Nrf2), but suppressed the stimulation of the NF-κB pathway caused by OVA. Conclusion. Taken together, our study suggests that schisandrin B attenuates the features of asthmatic lungs by inhibiting the NF-κB pathway and activating the Nrf2 signaling pathway.

scholarly journals Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT

2020 ◽  
Author(s):  
Zhijuan Xie ◽  
Haidan Sun ◽  
Xiaogang Li ◽  
Wei Sun ◽  
Jia Yin
Keyword(s):  
Allergic Asthma ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Birch Pollen ◽  
Control Group ◽  
Tandem Mass ◽  
Allergen Specific Immunotherapy ◽  
Before And After ◽  
Leukocyte Extravasation ◽  
Asthma Group

Abstract Subcutaneous immunotherapy (SCIT) is a classic form of allergen-specific immunotherapy that is used to treat birch pollen induced allergic asthma. To investigate the underlying molecular mechanisms of SCIT, we aimed to profile lung samples to explore changes in the differential proteome before and after SCIT in mice with allergic asthma. Fresh lungs were collected from three groups of mice: 1) control mice, 2) birch pollen-induced allergic mice and 3) birch pollen-induced allergic mice with SCIT. Tandem mass tag (TMT) labelling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze the lung proteome in the mice. Ingenuity pathway analysis (IPA) and Gene Ontology (GO) classification analysis were applied to identify differentially expressed proteins (DEPs) and crucial pathways. The screened DEPs were validated by immunohistochemistry analysis. A total of 317 proteins were upregulated and 184 proteins were downregulated in the asthma group compared to those of the control group. In contrast, 639 DEPs (163 upregulated and 456 downregulated proteins) were identified after SCIT in comparison with those of the asthma group. Among the 639 DEPs, 277 proteins returned to similar levels as those of the relative non-asthma condition. Bioinformatic analysis revealed that the 277 proteins played a significant role in the leukocyte extravasation signaling pathway. Of note, this is the first report that Vav1 (proto-oncogene vav) may serve as a biomarker of birch pollen-specific SCIT in mice. The leukocyte extravasation signaling pathway and related DEPs were of crucial importance in birch pollen SCIT.

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