Characterization of endoglucanases from the brown rot fungi Gloeophyllum sepiarium and Gloeophyllum trabeum

1998 ◽  
Vol 23 (1-2) ◽  
pp. 133-140 ◽  
Author(s):  
S.D Mansfield ◽  
J.N Saddler ◽  
G.M Gübitz
Keyword(s):  
Brown Rot ◽  
Gloeophyllum Trabeum ◽  
Brown Rot Fungi

Comparative Studies on Thermochemical Characterization of Corn Stover Pretreated by White-Rot and Brown-Rot Fungi

2011 ◽  
Vol 59 (18) ◽  
pp. 9965-9971 ◽  
Author(s):  
Yelin Zeng ◽  
Xuewei Yang ◽  
Hongbo Yu ◽  
Xiaoyu Zhang ◽  
Fuying Ma
Keyword(s):  
Corn Stover ◽  
Comparative Studies ◽  
Brown Rot ◽  
White Rot ◽  
Brown Rot Fungi

Changes of EPR Spectra of Wood Impregnated with Copper-Based Preservatives during Exposure to Several Wood-Rotting Fungi

Holzforschung ◽  
2002 ◽  
Vol 56 (3) ◽  
pp. 229-238 ◽  
Author(s):  
M. Humar ◽  
M. Petrič ◽  
F. Pohleven ◽  
M. Šentjurc ◽  
P. Kalan
Keyword(s):  
Schizophyllum Commune ◽  
White Rot Fungi ◽  
Brown Rot ◽  
White Rot ◽  
Gloeophyllum Trabeum ◽  
Nutrient Media ◽  
Paramagnetic Resonance ◽  
Copper Oxalate ◽  
Brown Rot Fungi ◽  
Electron Paramagnetic

SummaryThe tolerance of various fungi against copper was examined. For this purpose, we impregnated Norway spruce (Picea abies) specimens with two different aqueous solutions: copper(II) octanoate with ethanolamine or copper(II) sulfate (cCu= 1.0 × 10−2mol/l). Impregnated and unimpregnated test specimens were then exposed to brown rot fungiAntrodia vaillantiiandGloeophyllum trabeumor to white-rot fungiSchizophyllum communeandTrametes versicolor. After 2, 4, 6 and 12 weeks of exposure Electron Paramagnetic Resonance, Atomic Absorption Spectroscopy and mass loss measurements were performed. The results indicate thatA. vaillantii, G. trabeumandT. versicolortransform copper(II) sulfate in wood into non-soluble, and therefore non-toxic, copper oxalate. The intensity of this reaction depends on the amount of excreted oxalic acid and was the highest forA. vaillantiiand the lowest forT. versicolor. In the presence of ethanolamine, formation of insoluble copper oxalate was not possible and therefore, decay could not proceed. The major portion of copper remained in the wood and only minor amounts were in some cases translocated into nutrient media.

scholarly journals Distribution, Characteristics, and Regulatory Potential of Long Noncoding RNAs in Brown-Rot Fungi

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Alessandra Borgognone ◽  
Walter Sanseverino ◽  
Riccardo Aiese Cigliano ◽  
Raúl Castanera
Keyword(s):  
Noncoding Rnas ◽  
Gc Content ◽  
Brown Rot ◽  
Long Noncoding Rnas ◽  
Upstream Gene ◽  
Coniophora Puteana ◽  
Brown Rot Fungi ◽  
Serpula Lacrymans ◽  
Genomic Regions

Long noncoding RNAs have been thoroughly studied in plants, animals, and yeasts, where they play important roles as regulators of transcription. Nevertheless, almost nothing is known about their presence and characteristics in filamentous fungi, especially in basidiomycetes. In the present study, we have carried out an exhaustive annotation and characterization of lncRNAs in two lignin degrader basidiomycetes, Coniophora puteana and Serpula lacrymans. We identified 2,712 putative lncRNAs in the former and 2,242 in the latter, mainly originating from intergenic locations of transposon-sparse genomic regions. The lncRNA length, GC content, expression levels, and stability of the secondary structure differ from coding transcripts but are similar in these two species and resemble that of other eukaryotes. Nevertheless, they lack sequence conservation. Also, we found that lncRNAs are transcriptionally regulated in the same proportion as genes when the fungus actively decomposes soil organic matter. Finally, up to 7% of the upstream gene regions of Coniophora puteana and Serpula lacrymans are transcribed and produce lncRNAs. The study of expression trends in these gene-lncRNA pairs uncovered groups with similar and opposite transcriptional profiles which may be the result of cis-transcriptional regulation.

Influence of carbon source on wood decay-associated gene expression in sequential hyphal zones of the brown rot fungus Gloeophyllum trabeum

2021 ◽  
Author(s):  
Kiwamu Umezawa ◽  
Shuji Itakura
Keyword(s):  
Gene Expression ◽  
Gene Regulation ◽  
Carbon Source ◽  
Glycoside Hydrolase ◽  
Degradation Mechanism ◽  
Enzymatic Saccharification ◽  
Brown Rot ◽  
Gloeophyllum Trabeum ◽  
Brown Rot Fungi ◽  
Brown Rot Fungus

Abstract Brown rot fungi show a two-step wood degradation mechanism comprising oxidative radical-based and enzymatic saccharification systems. Recent studies have demonstrated that the brown rot fungus Rhodonia placenta expresses oxidoreductase genes ahead of glycoside hydrolase genes and spatially protects the saccharification enzymes from oxidative damage of the oxidoreductase reactions. This study aimed to assess the generality of the spatial gene regulation of these genes in other brown rot fungi and examine the effects of carbon source on the gene regulation. Gene expression analysis was performed on 14 oxidoreductase and glycoside hydrolase genes in the brown rot fungus Gloeophyllum trabeum, directionally grown on wood, sawdust-agar, and glucose-agar wafers. In G. trabeum, both oxidoreductase and glycoside hydrolase genes were expressed at higher levels in sections behind the wafers. The upregulation of glycoside hydrolase genes was significantly higher in woody substrates than in glucose, whereas the oxidoreductase gene expression was not affected by substrates.

scholarly journals Design and synthesis of new benzofuran-1,2,3-triazole hybrid preservatives and the evaluation of their antifungal potential against white and brown-rot fungi

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 7828-7843
Author(s):  
Fahimeh Abedinifar ◽  
S. Morteza F. Farnia ◽  
Seyyed Khalil Hosseinihashemi ◽  
Abbas Jalaligoldeh ◽  
Shahrbanoo Arabahmadi ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Brown Rot ◽  
White Rot ◽  
Gloeophyllum Trabeum ◽  
Coniophora Puteana ◽  
Brown Rot Fungi ◽  
Design And Synthesis ◽  
Poria Placenta ◽  
Antifungal Potential ◽  
Antifungal Effects

A series of novel benzofuran-1,2,3-triazole hybrids were synthesized and investigated as fungicidal preservatives. The compounds were evaluated for their antifungal potential against white-rot (Trametes versicolor), dry brown-rot (Poria placenta), and wet brown-rot (Coniophora puteana and Gloeophyllum trabeum) fungi, at different concentrations (500 ppm and 1000 ppm). The tests of the final products (8a, 8b, 8c, 8d, 8e, 8f, and 8g) demonstrated that compound N-((1-(4-fluorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)benzofuran-2-carboxamide (8f) at a concentration of 500 ppm was the most active against wet brown-rot C. puteana (23.86% inhibition) and G. trabeum (47.16% inhibition) fungi. However, testing demonstrated that compounds 8a, 8b, 8c, 8d, and 8g at a concentration of 500 ppm did not exhibit acceptable antifungal effects against white-rot T. versicolor and dry brown-rot P. placenta fungi.

scholarly journals Transcriptome analysis of the brown rot fungus Gloeophyllum trabeum during lignocellulose degradation

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243984
Author(s):  
Kiwamu Umezawa ◽  
Mai Niikura ◽  
Yuka Kojima ◽  
Barry Goodell ◽  
Makoto Yoshida
Keyword(s):  
Degradation Mechanism ◽  
Enzymatic Saccharification ◽  
Japanese Cedar ◽  
Brown Rot ◽  
Terpene Synthase ◽  
Lignocellulose Degradation ◽  
Substrate Selectivity ◽  
Gloeophyllum Trabeum ◽  
Wood Degradation ◽  
Brown Rot Fungi

Brown rot fungi have great potential in biorefinery wood conversion systems because they are the primary wood decomposers in coniferous forests and have an efficient lignocellulose degrading system. Their initial wood degradation mechanism is thought to consist of an oxidative radical-based system that acts sequentially with an enzymatic saccharification system, but the complete molecular mechanism of this system has not yet been elucidated. Some studies have shown that wood degradation mechanisms of brown rot fungi have diversity in their substrate selectivity. Gloeophyllum trabeum, one of the most studied brown rot species, has broad substrate selectivity and even can degrade some grasses. However, the basis for this broad substrate specificity is poorly understood. In this study, we performed RNA-seq analyses on G. trabeum grown on media containing glucose, cellulose, or Japanese cedar (Cryptomeria japonica) as the sole carbon source. Comparison to the gene expression on glucose, 1,129 genes were upregulated on cellulose and 1,516 genes were upregulated on cedar. Carbohydrate Active enZyme (CAZyme) genes upregulated on cellulose and cedar media by G. trabeum included glycoside hyrolase family 12 (GH12), GH131, carbohydrate esterase family 1 (CE1), auxiliary activities family 3 subfamily 1 (AA3_1), AA3_2, AA3_4 and AA9, which is a newly reported expression pattern for brown rot fungi. The upregulation of both terpene synthase and cytochrome P450 genes on cedar media suggests the potential importance of these gene products in the production of secondary metabolites associated with the chelator-mediated Fenton reaction. These results provide new insights into the inherent wood degradation mechanism of G. trabeum and the diversity of brown rot mechanisms.

Detection of iron-chelating and iron-reducing compounds in four brown rot fungi

Holzforschung ◽  
2013 ◽  
Vol 67 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Anne Christine Steenkjær Hastrup ◽  
Trine Østergaard Jensen ◽  
Bo Jensen
Keyword(s):  
Agar Plate ◽  
Brown Rot ◽  
Growth Media ◽  
Gloeophyllum Trabeum ◽  
Decayed Wood ◽  
Brown Rot Fungi ◽  
Reducing Activity ◽  
Serpula Lacrymans ◽  
Chelating Compounds ◽  
Reducing Capacity

Abstract The presence of iron-chelating and iron-reducing compounds has been evaluated qualitatively and quantitatively in the four brown rot fungi Meruliporia incrassata (M. incrassata), Gloeophyllum trabeum (G. trabeum), Coniophora puteana (C. puteana) and Serpula lacrymans (S. lacrymans). Samples of actively growing mycelium from liquid growth media, decayed wood, and agar plate cultures were in focus. Iron-chelating compounds were found in all four species, with the highest reactivity in G. trabeum and S. lacrymans, and the lowest in M. incrassata. Iron-reducing activity, measured in the liquid medium, was found in all four fungi. However, in wood extractions, S. lacrymans was not effective in this regard, although the agar grown mycelium of this fungus showed the highest iron-reducing capacity of the four. The presence of both catecholate and hydroxamate chelators was detected in all four species. G. trabeum showed the highest concentration overall of extracellular chelators, including both catecholate and hydroxamate derivatives.

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