Single-cell volume estimation by three-dimensional wide-field microscopy applied to astroglial primary cultures

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

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Correlation between tympanometry volume and three-dimensional computed tomography mastoid volumetry in tympanoplasty candidates

The Journal of Laryngology & Otology ◽

10.1017/s002221512100164x ◽

2021 ◽

pp. 1-5

Author(s):

L Epprecht ◽

L Qingsong ◽

N Stenz ◽

S Hashimi ◽

T Linder

Keyword(s):

Computed Tomography ◽

Tympanic Membrane ◽

Cell Volume ◽

Radiation Injury ◽

Three Dimensional ◽

Non Invasive ◽

Tympanic Membrane Perforations ◽

Computed Tomography Scans ◽

Air Cells ◽

Mastoid Air Cells

Abstract Objective Ventilation of the middle ear and mastoid air cells is believed to play an important role in the pathogenesis of chronic ear disease. Traditionally, ventilation is assessed by computed tomography. However, this exposes patients to cumulative radiation injury. In cases with a perforation in the tympanic membrane, tympanometry potentially presents a non-invasive alternative to measure the ventilated middle-ear and mastoid air cell volume. This study hypothesised that total tympanometry volume correlates with ventilated middle-ear and mastoid air cell volume. Method Total tympanometry volume was compared with ventilated middle-ear and mastoid air cell volume on computed tomography scans in 20 tympanic membrane perforations. Results There was a high correlation between tympanometry and computed tomography volumes (r = 0.78; p < 0.001). A tympanometry volume more than 2 ml predicted good ventilation on computed tomography. Conclusion These results may help reduce the need for pre-operative computed tomography in uncomplicated cases with tympanic membrane perforations.

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Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients

Rheumatology International ◽

10.1007/s00296-003-0333-0 ◽

2004 ◽

Vol 24 (2) ◽

pp. 71-76 ◽

Cited By ~ 1

Author(s):

Samuel Solomon ◽

Madhan Masilamani ◽

Subhasis Mohanty ◽

J�rg E. Schwab ◽

Eva-Maria Boneberg ◽

...

Keyword(s):

Synovial Fluid ◽

Single Cell ◽

Three Dimensional ◽

Cell Suspensions ◽

Synovial Fluid Cells

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Inferring Single-Cell 3D Chromosomal Structures Based on the Lennard-Jones Potential

International Journal of Molecular Sciences ◽

10.3390/ijms22115914 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5914

Author(s):

Mengsheng Zha ◽

Nan Wang ◽

Chaoyang Zhang ◽

Zheng Wang

Keyword(s):

Single Cell ◽

Loss Function ◽

Gaussian Function ◽

Three Dimensional ◽

Lennard Jones Potential ◽

Jones Potential ◽

Cubic Lattice ◽

Lennard Jones ◽

3D Fish ◽

Well Depth

Reconstructing three-dimensional (3D) chromosomal structures based on single-cell Hi-C data is a challenging scientific problem due to the extreme sparseness of the single-cell Hi-C data. In this research, we used the Lennard-Jones potential to reconstruct both 500 kb and high-resolution 50 kb chromosomal structures based on single-cell Hi-C data. A chromosome was represented by a string of 500 kb or 50 kb DNA beads and put into a 3D cubic lattice for simulations. A 2D Gaussian function was used to impute the sparse single-cell Hi-C contact matrices. We designed a novel loss function based on the Lennard-Jones potential, in which the ε value, i.e., the well depth, was used to indicate how stable the binding of every pair of beads is. For the bead pairs that have single-cell Hi-C contacts and their neighboring bead pairs, the loss function assigns them stronger binding stability. The Metropolis–Hastings algorithm was used to try different locations for the DNA beads, and simulated annealing was used to optimize the loss function. We proved the correctness and validness of the reconstructed 3D structures by evaluating the models according to multiple criteria and comparing the models with 3D-FISH data.

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Changes in Astroglial Cell Volume after Exposure to HgCl2 or CH3HgCl

Alternatives to Laboratory Animals ◽

10.1177/026119299202000212 ◽

1992 ◽

Vol 20 (2) ◽

pp. 246-250

Author(s):

Lars Rönnbäck ◽

Elisabeth Hansson

Keyword(s):

Glucose Uptake ◽

Cell Volume ◽

Receptor Agonist ◽

Primary Cultures ◽

Control Cell ◽

Astroglial Cell ◽

Receptor Stimulation ◽

Adrenoceptor Agonists ◽

Β Receptor ◽

Induced Changes

Cell volume was determined by measuring [14C]-3- O-methyl glucose uptake in astroglial-enriched primary cultures. Control cell volume was 3.20μl/mg protein. After incubation in 10 5M HgCl2 for 60 minutes, there was a 71% increase in cell volume. This increase was partially inhibited in the presence of the α1 receptor agonist, phenylephrine, or by the α2 receptor agonist clonidine, and was completely reversible by their respective antagonists, prazosine and yohimbine. The β receptor agonist, isoproterenol, which in itself increased cell volume, and 5-hydroxytryptamine (5HT) did not affect the HgCl2-induced changes in cell volume. 10 5M CH3HgCl increased cell volume by 26% after 30 minutes of incubation. This increase was not significantly influenced by adrenoceptor agonists or 5HT. It therefore seems that mercurial-induced changes in cell volume can be regulated by astroglial receptor stimulation.

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