Differential cytokine mRNA expression in swine whole blood and peripheral blood mononuclear cell cultures

2001 ◽  
Vol 79 (1-2) ◽  
pp. 41-52 ◽  
Author(s):  
Haile Yancy ◽  
Sherry L. Ayers ◽  
Dorothy E. Farrell ◽  
Agnes Day ◽  
Michael J. Myers
Keyword(s):  
Peripheral Blood Mononuclear Cell ◽  
Mrna Expression ◽  
Mononuclear Cell ◽  
Cell Cultures ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Cytokine Mrna ◽  
Peripheral Blood Mononuclear ◽  
Cytokine Mrna Expression

scholarly journals Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ling Lin ◽  
Yong-Song Yue ◽  
Ni-Dan Wang ◽  
Lei-Yan Wei ◽  
Yang Han ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Antiviral Treatment ◽  
Quantitative Detection ◽  
Peripheral Blood Mononuclear ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Hiv 1

Abstract Background A more time saving, convenient, reproducible, and scalable method is needed to assess total HIV-1 DNA levels. Methods Frozen whole blood and peripheral blood mononuclear cell (PBMC) samples both 200 μl at the same point were used to detect total HIV-1 DNA. Automatic extraction of total HIV-1 DNA was used to ensure the consistency of sample extraction efficiency. The detection reagent was HIV-1 DNA quantitative detection kit and real-time quantitative PCR was utilized. Results Of the 44 included patients, 42 were male and 2 were female, with a median age of 33 years. Thirty-three cases were collected after receiving antiviral treatment, with a median duration of treatment of 3 months, and the other 11 cases were collected before antiviral treatment. The median viral load was 1.83 log10 copies/mL, the median CD4 and CD8 count were 94 and 680 cells/μL, and the median CD4/CD8 ratio was 0.18. The results of the two samples were 3.02 ± 0.39 log10 copies/106 PBMCs in PBMC samples and 3.05 ± 0.40 log10 copies/106 PBMCs in whole blood samples. The detection results of the two methods were highly correlated and consistent by using paired t test (P = 0.370), pearson correlation (r = 0.887, P < 0.0001) and intra-group correlation coefficient (ICC = 0.887, P < 0.0001) and bland-altman [4.55% points were outside the 95% limits of agreement (− 0.340 ~ 0.390)]. Conclusions The results of the whole blood sample test for total HIV-1 DNA are consistent with those of PBMC samples. In a clinical setting it is recommended to use whole blood samples directly for the evaluation of the HIV reservoir.

Altered cytokine release in peripheral blood mononuclear cell cultures from patients with the chronic fatigue syndrome

Cytokine ◽  
1991 ◽  
Vol 3 (4) ◽  
pp. 292-298 ◽  
Author(s):  
Chun C. Chao ◽  
Edward N. Janoff ◽  
Shuxian Hu ◽  
Kelly Thomas ◽  
Michael Gallagher ◽  
...  
Keyword(s):  
Chronic Fatigue Syndrome ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Cell Cultures ◽  
Peripheral Blood ◽  
Chronic Fatigue ◽  
Cytokine Release ◽  
Peripheral Blood Mononuclear ◽  
Fatigue Syndrome

Morphine inhibits the release of tumor necrosis factor in human peripheral blood mononuclear cell cultures

1993 ◽  
Vol 15 (3) ◽  
pp. 447-453 ◽  
Author(s):  
Chun C. Chao ◽  
Thomas W. Molitor ◽  
Karen Close ◽  
Shuxian Hu ◽  
Phillip K. Peterson
Keyword(s):  
Tumor Necrosis Factor ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Cell Cultures ◽  
Peripheral Blood ◽  
Tumor Necrosis ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Necrosis Factor

Osteoclastogenesis in peripheral blood mononuclear cell cultures of periprosthetic osteolysis patients and the phenotype of T cells localized in periprosthetic tissues

Biomaterials ◽  
2010 ◽  
Vol 31 (29) ◽  
pp. 7519-7525 ◽  
Author(s):  
Ilaria Roato ◽  
Davide Caldo ◽  
Lucia D’Amico ◽  
Patrizia D’Amelio ◽  
Laura Godio ◽  
...  
Keyword(s):  
T Cells ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Cell Cultures ◽  
Peripheral Blood ◽  
Periprosthetic Osteolysis ◽  
Peripheral Blood Mononuclear

Cell density-dependent proliferation in frequently-fed peripheral blood mononuclear cell cultures

Cytotherapy ◽  
2000 ◽  
Vol 2 (4) ◽  
pp. 267-280 ◽  
Author(s):  
S.D. Patel ◽  
W.M. Miller ◽  
J.N. Winter ◽  
E.T. Papoutsakis
Keyword(s):  
Peripheral Blood Mononuclear Cell ◽  
Cell Density ◽  
Mononuclear Cell ◽  
Cell Cultures ◽  
Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Density Dependent
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