Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica acquires resistance to salt stress in transgenic tobacco plants

Plant Science ◽  
1999 ◽  
Vol 146 (2) ◽  
pp. 81-88 ◽  
Author(s):  
M Sugino ◽  
T Hibino ◽  
Y Tanaka ◽  
N Nii ◽  
T Takabe ◽  
...  
2020 ◽  
Vol 21 (4) ◽  
pp. 1323 ◽  
Author(s):  
Wei Li ◽  
Changxi Dang ◽  
Yuxiu Ye ◽  
Zunxin Wang ◽  
Laibao Hu ◽  
...  

In plants, auxin/indoleacetic acid (Aux/IAA) proteins are transcriptional regulators that regulate developmental process and responses to phytohormones and stress treatments. However, the regulatory functions of the Vitis vinifera L. (grapevine) Aux/IAA transcription factor gene VvIAA18 have not been reported. In this study, the VvIAA18 gene was successfully cloned from grapevine. Subcellular localization analysis in onion epidermal cells indicated that VvIAA18 was localized to the nucleus. Expression analysis in yeast showed that the full length of VvIAA18 exhibited transcriptional activation. Salt tolerance in transgenic tobacco plants and Escherichia. coli was significantly enhanced by VvIAA18 overexpression. Real-time quantitative PCR analysis showed that overexpression of VvIAA18 up-regulated the salt stress-responsive genes, including pyrroline-5-carboxylate synthase (NtP5CS), late embryogenesis abundant protein (NtLEA5), superoxide dismutase (NtSOD), and peroxidase (NtPOD) genes, under salt stress. Enzymatic analyses found that the transgenic plants had higher SOD and POD activities under salt stress. Meanwhile, component analysis showed that the content of proline in transgenic plants increased significantly, while the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) decreased significantly. Based on the above results, the VvIAA18 gene is related to improving the salt tolerance of transgenic tobacco plants. The VvIAA18 gene has the potential to be applied to enhance plant tolerance to abiotic stress.


2009 ◽  
Vol 46 (2) ◽  
pp. 63-75 ◽  
Author(s):  
Roya Razavizadeh ◽  
Ali Ehsanpour

Effects of salt stress on proline content, expression of delta-1-pyrroline-5-carboxylate synthetase, and activities of catalase and ascorbate peroxidase in transgenic tobacco plantsIn arid and semiarid regions, soil salinity limits crop production. Proline accumulation in transgenic plants results in increased stress tolerance, but the underlying mechanism was unclear. To elucidate it, effects of salt stress on the expression pattern of Δ1-pyrroline-5-carboxylate synthetase (P5CS), proline content, catalase (CAT), and ascorbate peroxidase (APX) activities were analyzed in transgenic tobacco (Nicotiana tabacumcv. Wisconsin). Transgenic tobacco plants containing CaMV 35S promoter and theP5CSgene from moth bean (Vigna aconitifolia), linked to theNPTIIgene, were culturedin vitrowith or without 300 mM NaCl. The expression pattern ofP5CSwas evaluated using semiquantitative RT-PCR (reverse transcription-polymerase chain reaction). Time-course experiments showed an increase in proline content after 4 h of the treatment. The level ofP5CStranscripts was increased significantly in leaves and roots of transgenic plants after 24 and 48 h of treatment. This rise in transcripts was concomitant with the highest increase in proline content. In addition, CAT and APX activities increased under salt stress, and their highest activities were observed after 24 and 48 h of NaCl treatment. These results suggest thatP5CSis an inducible gene regulating the activities of CAT and APX and the accumulation of proline in plants subjected to salt stress.


PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23776 ◽  
Author(s):  
Kevin Begcy ◽  
Eduardo D. Mariano ◽  
Lucia Mattiello ◽  
Alessandra V. Nunes ◽  
Paulo Mazzafera ◽  
...  

Author(s):  
Qiang Guo ◽  
Xiaoxia Tian ◽  
Peichun Mao ◽  
Lin Meng

Na+ compartmentalization into vacuoles is one of the effective strategies for adaptation of halophytes to saline environments. The tonoplast Na+/H+ antiporter (NHX) has been proved to be involved in the compartmentalization of Na+ into vacuoles to alleviate Na+ toxicity in cytoplasm. However, the function of NHX in halophyte Iris lactea is still unclear under salt stress. In this study, a significant positive correlation was observed between Na+ accumulations and IlNHX expression levels in shoots and roots under different concentrations of NaCl (0-200 mM), indicating IlNHX might be involved in Na+ accumulation of I. lactea in response to salt stress. More important, IlNHX was specifically localized to the tonoplast. Transgenic tobacco plants expressing IlNHX grew better and showed higher salinity tolerance under salt (200 mM NaCl) stress than those of wild type (WT) plants. Compared to WT plants, transgenic tobacco plants accumulated more Na+ and K+ and maintained higher K+/Na+ ratios in tissues by salt stress, accompanied by the reduction of chlorophyll loss and lipid peroxidation in the presence of salt. Interestingly, we found that transgenic tobacco plants exhibited markedly higher tonoplast H+-ATPase activity relative to WT plants subjected to salt. Overall, overexpression of IlNHX in tobacco could compartmentalize excessive Na+ into vacuoles to keep the cytosolic K+/Na+ balance by enhanced tonoplast proton pumps activity, which would be contributed to maintain K+ and Na+ homeostasis, to improve photosynthesis efficiency and to protect cell membrane integrity under salt stress.


Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1749
Author(s):  
Samuel Aduse Poku ◽  
Peter Nkachukwu Chukwurah ◽  
Htut Htet Aung ◽  
Ikuo Nakamura

Climate change, with its attendant negative effects, is expected to hamper agricultural production in the coming years. To counteract these negative effects, breeding of environmentally resilient plants via conventional means and genetic engineering is necessary. Stress defense genes are valuable tools by which this can be achieved. Here we report the successful cloning and functional characterization of a melon Y3SK2-type dehydrin gene, designated as CmLEA-S. We generated CmLEA-S overexpressing transgenic tobacco lines and performed in vitro and in vivo drought and salt stress analyses. Seeds of transgenic tobacco plants grown on 10% polyethylene glycol (PEG) showed significantly higher germination rates relative to wild-type seeds. In the same way, transgenic seeds grown on 150 mM sodium chloride (NaCl) recorded significantly higher germination percentages compared with wild-type plants. The fresh weights and root lengths of young transgenic plants subjected to drought stress were significantly higher than that of wild-type plants. Similarly, the fresh weights and root lengths of transgenic seedlings subjected to salt stress treatments were also significantly higher than wild-type plants. Moreover, transgenic plants subjected to drought and salt stresses in vivo showed fewer signs of wilting and chlorosis, respectively. Biochemical assays revealed that transgenic plants accumulated more proline and less malondialdehyde (MDA) compared with wild-type plants under both drought and salt stress conditions. Finally, the enzymatic activities of ascorbate peroxidase (APX) and catalase (CAT) were enhanced in drought- and salt-stressed transgenic lines. These results suggest that the CmLEA-S gene could be used as a potential candidate gene for crop improvement.


2014 ◽  
Vol 38 ◽  
pp. 268-280 ◽  
Author(s):  
Rahma JARDAK JAMOUSSI ◽  
Mohamed Malek ELABBASSI ◽  
Hatem BEN JOUIRA ◽  
Mohsen HANANA ◽  
Néjia ZOGHLAMI ◽  
...  

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