Genetic differences of Ascaridia galli egg output in laying hens following a single dose infection

Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolkABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A – F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.

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Decreasing half-life of dieldrin in egg yolk following a single oral administration of aldrin to laying hens

Acta Veterinaria Hungarica ◽

10.1556/004.49.2001.4.10 ◽

2001 ◽

Vol 49 (4) ◽

pp. 465-472

Author(s):

N. Furusawa

Keyword(s):

Body Weight ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Single Dose ◽

Half Life ◽

High Performance ◽

Laying Hens ◽

Egg Yolk ◽

Single Oral Administration ◽

Low Levels

Laying hens were treated orally with a single dose of aldrin (AD) 1 mg/kg body weight. Concentrations (μg/g) of AD or its epoxide (= dieldrin, DD) in the yolk of eggs laid for 21 days after AD treatment were determined by normalphase high-performance liquid chromatography. The limits of determination were 0.02 μg/g for AD and 0.03 μg/g for DD, respectively. After AD treatment, although the low levels of AD (mean 0.02–0.03 μg/g) were observed only during a three-day period (from 4th to 6th days), DD (mean 0.15 μg/g) was found already on the 2nd day, indicating that the epoxidation of AD to DD in the hen’s body is rapid. The highest level of DD (mean 0.40 μg/g) was detected on the 6th day, and then DD levels decreased slowly and were detected up to the 21st day. In this decreasing phase, the half-life of DD in the yolk was estimated to be 25.6 days with a 95% confidence interval from 22.7 to 29.4 days.

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Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v1i2.3129 ◽

2007 ◽

Vol 1 (2) ◽

Author(s):

Ummu Balqis ◽

Risa Tiuria ◽

Bambang Pontjo Priosoeryanto ◽

Darmawi D

Keyword(s):

Adult Worm ◽

Laying Hens ◽

Infective Larva ◽

Periodic Acid ◽

Goblet Cells ◽

Ascaridia Galli ◽

Defence Mechanisms ◽

Periodic Acid Schiff ◽

Post Immunization ◽

Pas Staining

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380µg of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehost’s defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells

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