Testing of SOS induction of artificial polycyclic musk fragrances in E. coli PQ37 (SOS chromotest)

1998 ◽  
Vol 95 (3) ◽  
pp. 147-154 ◽  
Author(s):  
V Mersch-Sundermann ◽  
S Kevekordes ◽  
C Jenter
Keyword(s):  
E Coli ◽  
Sos Induction ◽  
Sos Chromotest ◽  
Polycyclic Musk ◽  
Musk Fragrances

scholarly journals Nucleoside Analogues Are Potent Inducers of Pol V-mediated Mutagenesis

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 843
Author(s):  
Balagra Kasim Sumabe ◽  
Synnøve Brandt Ræder ◽  
Lisa Marie Røst ◽  
Animesh Sharma ◽  
Eric S. Donkor ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Mutation Frequency ◽  
Nucleoside Analogues ◽  
Cancer Therapies ◽  
E Coli ◽  
Replicative Stress ◽  
Pol V ◽  
Dna And Rna ◽  
Sos Induction ◽  
Anti Cancer

Drugs targeting DNA and RNA in mammalian cells or viruses can also affect bacteria present in the host and thereby induce the bacterial SOS system. This has the potential to increase mutagenesis and the development of antimicrobial resistance (AMR). Here, we have examined nucleoside analogues (NAs) commonly used in anti-viral and anti-cancer therapies for potential effects on mutagenesis in Escherichia coli, using the rifampicin mutagenicity assay. To further explore the mode of action of the NAs, we applied E. coli deletion mutants, a peptide inhibiting Pol V (APIM-peptide) and metabolome and proteome analyses. Five out of the thirteen NAs examined, including three nucleoside reverse transcriptase inhibitors (NRTIs) and two anti-cancer drugs, increased the mutation frequency in E. coli by more than 25-fold at doses that were within reported plasma concentration range (Pl.CR), but that did not affect bacterial growth. We show that the SOS response is induced and that the increase in mutation frequency is mediated by the TLS polymerase Pol V. Quantitative mass spectrometry-based metabolite profiling did not reveal large changes in nucleoside phosphate or other central carbon metabolite pools, which suggests that the SOS induction is an effect of increased replicative stress. Our results suggest that NAs/NRTIs can contribute to the development of AMR and that drugs inhibiting Pol V can reverse this mutagenesis.

SOS induction of selected naturally occurring substances in Escherichia coli (SOS chromotest)

1999 ◽  
Vol 445 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Sebastian Kevekordes ◽  
Volker Mersch-Sundermann ◽  
Christian M Burghaus ◽  
Jan Spielberger ◽  
Heinz H Schmeiser ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sos Induction ◽  
Naturally Occurring ◽  
Sos Chromotest

Polycyclic musk fragrances in human adipose tissue and human milk

Chemosphere ◽  
1996 ◽  
Vol 33 (10) ◽  
pp. 2033-2043 ◽  
Author(s):  
Gerhard G. Rimkus ◽  
Manfred Wolf
Keyword(s):  
Adipose Tissue ◽  
Human Milk ◽  
Human Adipose Tissue ◽  
Polycyclic Musk ◽  
Musk Fragrances

scholarly journals Genotoxicity of Some Metal-Based Antineoplastics, Evaluated by SOS Chromotest and Cytogenetic Analysis

1996 ◽  
Vol 3 (2) ◽  
pp. 91-99 ◽  
Author(s):  
Carmen Socaciu ◽  
Loan Pasca ◽  
Cristian Silvestru ◽  
Ionel Haiduc
Keyword(s):  
Chromosomal Aberrations ◽  
Cytogenetic Analysis ◽  
Positive Response ◽  
Metabolic Activation ◽  
Antineoplastic Drugs ◽  
Mutagenic Potential ◽  
E Coli ◽  
Bacterial Cultures ◽  
Sos Chromotest ◽  
Clastogenic Effect

The paper reports the screening results of two metal-based antineoplastic drugs with mutagenic potential, such as Romcis (trademark of Cisplatinum, produced in Romania) and diphenylantimony(III) diisopropyldithiophosphate (PADTF). Their effects were compared with those induced by Cyclophosphamide. Two mutagenicity tests, the SOS Chromotest and cytogenetic analysis were applied. The tests were carried out with or without metabolic activation (addition of S9-mix), either in E. coli PQ 37 cultures, using four doses (0.3, 3, 30 and 300 pmol compound/assay) for the SOS Chromotest or in leukocyte cultures using 0.3 mM from each compound, for cytogenetics. The dose- response relationships and SOSIP values revealed an indirect mutagenic potential for Cyclophosphamide, amplified by S9 mix in bacterial cultures and an antiproliferative, clastogenic effect on lymphocytes. For Romcis and diphenylantimony(III) diisopropyldithiophosphate, a significant positive response by SOS Chromotest was recorded, which correlated with increased frequencies of chromosomal aberrations.

Polycyclic musk fragrances in different environmental compartments in Berlin (Germany)

2001 ◽  
Vol 35 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Hermann Fromme ◽  
Thomas Otto ◽  
Konstanze Pilz
Keyword(s):  
Polycyclic Musk ◽  
Musk Fragrances ◽  
Environmental Compartments

In vitro genotoxicity of polycyclic musk fragrances in the micronucleus test

1997 ◽  
Vol 395 (2-3) ◽  
pp. 145-150 ◽  
Author(s):  
Sebastian Kevekordes ◽  
Volker Mersch-Sundermann ◽  
Martin Diez ◽  
Hartmut Dunkelberg
Keyword(s):  
Micronucleus Test ◽  
Polycyclic Musk ◽  
Musk Fragrances ◽  
In Vitro Genotoxicity

Study on the Interaction of Ions of Transient Metals with Ascorbic Acid in the Presence of Different Scavengers of Active Oxygen Species in SOS Chromotes

2002 ◽  
Vol 72 (2) ◽  
pp. 85-89
Author(s):  
Jadwiga Marczewska ◽  
Jadwiga H. Koziorowska
Keyword(s):  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Copper Ions ◽  
Active Oxygen Species ◽  
Active Oxygen ◽  
Oxygen Species ◽  
E Coli ◽  
Free Ions ◽  
Sos Chromotest ◽  
Transient Metals

SOS chromotest was employed to study the interaction of ascorbic acid with free ions of transient metals in the presence of added catalase, superoxide dismutase or D-mannitol. Catalase diminished the genotoxic activity of the mixture of ascorbic acid with copper ions in E. coli strains PQ37 and PQ 300, but genotoxicity of this mixture was not suppressed by superoxide dismutase and D-mannitol. The results suggest that copper ions diminished the content of peroxide generated by ascorbic acid.

scholarly journals Adherence to abiotic surface induces SOS response in Escherichia coli K-12 strains under aerobic and anaerobic conditions

Microbiology ◽  
2014 ◽  
Vol 160 (9) ◽  
pp. 1964-1973 ◽  
Author(s):  
Suelen B. Costa ◽  
Ana Carolina C. Campos ◽  
Ana Claudia M. Pereira ◽  
Ana Luiza de Mattos-Guaraldi ◽  
Raphael Hirata Júnior ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Wild Type Strain ◽  
Sos Response ◽  
Wild Type ◽  
Abiotic Surface ◽  
Anaerobic Conditions ◽  
E Coli ◽  
Endonuclease V ◽  
Sos Induction ◽  
K 12

During the colonization of surfaces, Escherichia coli bacteria often encounter DNA-damaging agents and these agents can induce several defence mechanisms. Base excision repair (BER) is dedicated to the repair of oxidative DNA damage caused by reactive oxygen species (ROS) generated by chemical and physical agents or by metabolism. In this work, we have evaluated whether the interaction with an abiotic surface by mutants derived from E. coli K-12 deficient in some enzymes that are part of BER causes DNA damage and associated filamentation. Moreover, we studied the role of endonuclease V (nfi gene; 1506 mutant strain) in biofilm formation. Endonuclease V is an enzyme that is involved in DNA repair of nitrosative lesions. We verified that endonuclease V is involved in biofilm formation. Our results showed more filamentation in the xthA mutant (BW9091) and triple xthA nfo nth mutant (BW535) than in the wild-type strain (AB1157). By contrast, the mutant nfi did not present filamentation in biofilm, although its wild-type strain (1466) showed rare filaments in biofilm. The filamentation of bacterial cells attaching to a surface was a consequence of SOS induction measured by the SOS chromotest. However, biofilm formation depended on the ability of the bacteria to induce the SOS response since the mutant lexA Ind− did not induce the SOS response and did not form any biofilm. Oxygen tension was an important factor for the interaction of the BER mutants, since these mutants exhibited decreased quantitative adherence under anaerobic conditions. However, our results showed that the presence or absence of oxygen did not affect the viability of BW9091 and BW535 strains. The nfi mutant and its wild-type did not exhibit decreased biofilm formation under anaerobic conditions. Scanning electron microscopy was also performed on the E. coli K-12 strains that had adhered to the glass, and we observed the presence of a structure similar to an extracellular matrix that depended on the oxygen tension. In conclusion, it was proven that bacterial interaction with abiotic surfaces can lead to SOS induction and associated filamentation. Moreover, we verified that endonuclease V is involved in biofilm formation.

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