A freeze-dried injectable form of flurbiprofen: development and optimisation using response surface methodology

1998 ◽  
Vol 161 (1) ◽  
pp. 87-94 ◽  
Author(s):  
K.A Kagkadis ◽  
D.M Rekkas ◽  
P.P Dallas ◽  
N.H Choulis
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Freeze Dried ◽  
Injectable Form

scholarly journals Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction

Marine Drugs ◽  
2018 ◽  
Vol 16 (8) ◽  
pp. 257 ◽  
Author(s):  
Marco Garcia-Vaquero ◽  
Gaurav Rajauria ◽  
Brijesh Tiwari ◽  
Torres Sweeney ◽  
John O’Doherty
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Laminaria Digitata ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Freeze Dried ◽  
Ultrasound Assisted

The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 °C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata.

Optimization of Antioxidant Extraction from Freeze-dried Pulp, Peel, and Seed of Burmese grape (Baccaurea ramiflora Lour.) by Response Surface Methodology

2021 ◽  
Author(s):  
Mohammad Afzal Hossain ◽  
Md. Sakib Hossain
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Time ◽  
Maximum Yield ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Solvent Concentration ◽  
Antioxidant Power ◽  
Freeze Dried

Abstract This study aimed to attain the optimum condition necessary for extracting the maximum yield of antioxidants from the freeze-dried pulp, peel, and seed of Burmese grape using response surface methodology (RSM). Solvent (ethanol) concentration (%), temperature (°C), and time (min) were taken as independent variables by factorial screening for the extraction procedure. After extraction, the antioxidant activity of all samples was determined employing 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic compounds (TPC), and ferric reducing antioxidant power (FRAP) assay. The experiment's optimum conditions were 80% solvent concentration, 69.01°C temperature, and 30 min for pulp. The optimum extraction conditions were found at 80°C for 29.39 min incubation time using 52.12% concentrated solvent for seed. For peel, the solvent concentration of 41.62% was found optimum when the temperature of 50°C and 30 min incubation time were used. The actual values of TPC, FRAP, and DPPH for freeze-dried pulp, peel and seed extracts were close to the predicted values, which confirms the models’ validity. The Analysis of Variance (ANOVA) showed that the models were significant for TPC, DPPH, and FRAP values of peel, pulp, and seed at different levels (p < 0.001 to p < 0.05). The composite desirability of pulp, seed, and peel were 0.94, 0.98, and 0.85, respectively, which suggest that the developed model could be effectively used for antioxidants’ extraction from freeze-dried pulp, peel, and seed of Burmese grape.

Optimization of the nanocellulose based cryoprotective medium to enhance the viability of freeze dried Lactobacillus plantarum using response surface methodology

LWT ◽  
2015 ◽  
Vol 64 (1) ◽  
pp. 326-332 ◽  
Author(s):  
Fatemeh Keivani Nahr ◽  
Reza Rezaei Mokarram ◽  
Mohammad Amin Hejazi ◽  
Babak Ghanbarzadeh ◽  
Mahmoud Sowti Khiyabani ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Lactobacillus Plantarum ◽  
Freeze Dried

scholarly journals Optimization of Extraction Conditions for Antioxidant Activity of Acer tegmentosum Using Response Surface Methodology

2021 ◽  
Vol 11 (3) ◽  
pp. 1134
Author(s):  
Inyong Kim ◽  
Jung-Heun Ha ◽  
Yoonhwa Jeong
Keyword(s):  
Hydrogen Peroxide ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Hepg2 Cells ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Freeze Dried ◽  
Hydrothermal Extraction

Extraction from edible plants is a highly important process that has various biological functions. To maximize biological activity, extraction methods should facilitate optimal extraction of functional phytochemicals. In this study, the optimal hydrothermal extraction conditions of Acer tegmentosum were determined using response surface methodology (RSM), and HepG2 cells were treated with optimized extract and hydrogen peroxide. In a central composition design, the independent variables were extraction temperature (X1: 70–90 °C), extraction time (X2: 2–6 h), and solvent-to-solid ratio (X3: 50–150). The maximum total phenolic contents (276.70 ± 10.11 mg GAE/g) and DPPH (2,2-diphenyl-1-pictylhydrazyl) activity (33.45 ± 2.20%) of A. tegmentosum were estimated at optimized extraction conditions, as follows: X1: 89.34 °C, X2: 7.36 h, X3: 184.09. Using the calculated extraction conditions, functional phytochemicals were extracted by hydrothermal extraction and freeze-dried. A. tegmentosum treatment (>10 μg/mL) of HepG2 cells remarkably attenuated hydrogen-peroxide-inducible hepatic cellular death and reactive oxygen species production in vitro.

scholarly journals Combinatorial Effects of Protective Agents on Survival Rate of the Yeast Starter, Saccharomyces cerevisiae 88-4, after Freeze-Drying

2021 ◽  
Vol 9 (3) ◽  
pp. 613
Author(s):  
Young-Wook Chin ◽  
Saerom Lee ◽  
Hwan Hee Yu ◽  
Seung Jae Yang ◽  
Tae-Wan Kim
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Response Surface Methodology ◽  
Survival Rate ◽  
Response Surface ◽  
Freeze Drying ◽  
Skim Milk ◽  
Fermented Foods ◽  
Brewing Yeast ◽  
Protective Agents ◽  
Freeze Dried

A yeast starter is formulated for commercial practices, including storage and distribution. The cell viability of the yeast starter is one of the most important factors for manufacturing alcoholic beverages to ensure their properties during the fermentation and formulation processes. In this study, 64 potential protective agents were evaluated to enhance the survival rate of the brewing yeast Saccharomyces cerevisiae 88-4 after freeze-drying. In addition, the optimized combination of protective agents was assessed for long-term storage. Finally, response surface methodology was applied to investigate the optimal concentration of each protectant. Twenty of the 64 additives led to an increase in the survival rate of freeze-dried S. cerevisiae 88-4. Among the various combinations of protectants, four had a survival rate >95%. The combination of skim milk, maltose, and maltitol exhibited the best survival rate of 61% after 42 weeks in refrigerated storage, and the composition of protectants optimized by response surface methodology was 6.5–10% skim milk, 1.8–4.5% maltose, and 16.5–18.2% maltitol. These results demonstrated that the combination of multiple protectants could alleviate damage to yeasts during freeze-drying and could be applied to the manufacturing starters for fermented foods.

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