Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats

In the present study, the methanolic extract of Moringa oleifera leaves (MEMOL) was evaluated for antiobesity activity in rats. The antiobesity potential of MEMOL was studied against high fat diet-induced obesity (HFD) in rats. In this study, chronic administration of HFD in rats produced hypercholesterolemia (116.2 ± 0.27 mg/dL), which led to an increase in the body weight (225 gr), total cholesterol, triglycerides (263.0 ± 4.69 mg/dL), and attenuation in the levels of HDL (34.51 ± 2.20 mg/dL) as well as changes in body temperature of animals. Treatment of obese rats with MEMOL for 49 days resulted in a significant (P<0.001) change in body weight, total cholesterol, triglycerides, and LDL level along with a significant (P<0.001) increase in body temperature as compared to the HFD-induced obesity. MEMOL treated rats also showed a significant decrease in the level of liver biomarkers, organ weight, and blood glucose level. Further, rats treated with MEMOL (200 mg and 400 mg/kg) show reduced atherogenic index (1.7 ± 0.6 and 0.87 ± 0.76). The results indicate that the rats treated with Moringa oleifera (MO) have significantly attenuated the body weight without any change in the feed intake and also elicited significant thermogenic effect and to act as hypolipidemic and thermogenic property in obesity related disorders.

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Particular Matter of Motor Vehicle Exposure and High-Fat Diet Effects on Kidney Histopathology, Creatinine, and Malondialdehyde (MDA) Levels in Wistar Rats

Indonesian Journal of Environmental Management and Sustainability ◽

10.26554/ijems.2021.5.3.124-128 ◽

2021 ◽

Vol 5 (3) ◽

pp. 124-128

Author(s):

Rizka Veni ◽

Awal Prasetyo ◽

Muflihatul Muniroh

Keyword(s):

High Fat Diet ◽

Wistar Rats ◽

Motor Vehicle ◽

Histopathological Change ◽

Normal Diet ◽

Control Group ◽

High Fat ◽

Control Group Design ◽

Treatment Groups ◽

Significant Difference

This study aims to analyze the effect of combination of motor vehicle particular matter exposure and high-fat diet in kidney histopathology, creatinine levels, and MDA levels in Wistar rats. This study used a posttest-only control group design. Eighteen healthy male Wistar rats were divided into three groups. The intervention groups received motor vehicle fume exposure for 100 s with normal diet (X1) or high-fat diet (X2), and the control group received no exposure (C). Data analysis was processed with a SPSS 25.0 computer program by using the one-way ANOVA test followed by post hoc LSD. The degree of kidney histopathological damage showed significant differences between the X1 and X2 groups when compared with the control group (p < 0.05). The results of the creatinine level examination found a significant difference between the X2 and C groups (p < 0.05) and the treatment groups X1 and X2 (p < 0.05). The results of kidney MDA level examination showed a significant difference between the treatment groups (X1 and X2) and the control group (p < 0.05). The combination of particular matter of motor vehicle fumes exposure and high-fat diet could induce kidney damage through histopathological change and increased creatinine levels and kidney MDA levels in Wistar rats.

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Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet

Comparative Clinical Pathology ◽

10.1007/s00580-018-2828-9 ◽

2018 ◽

Vol 28 (5) ◽

pp. 1197-1203 ◽

Cited By ~ 7

Author(s):

Mohammad Mahdi Zangeneh ◽

Saman Salmani ◽

Akram Zangeneh ◽

Reza Khedri ◽

Mohammad Saeid Zarei

Keyword(s):

Aqueous Extract ◽

High Fat Diet ◽

Wistar Rats ◽

High Fat ◽

Biochemical Effects ◽

Tragopogon Graminifolius ◽

Liver Tissues

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Mangosteen Pericarp Inhibits Nuclear Factor ?B (NF-?B) Activation and Reduces Expression of ICAM-1 in High Cholesterol Diet Rat

Indonesian Journal of Cardiology ◽

10.30701/ijc.v34i1.302 ◽

2013 ◽

pp. 23-32

Author(s):

H Hendarto ◽

Mohammad Saifur Rohman ◽

Djanggan Sargowo

Keyword(s):

Crude Extract ◽

High Fat Diet ◽

Inhibitory Effect ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

High Fat ◽

Factor B ◽

Underlying Risk Factor ◽

Underlying Risk

Background: Atherosclerosis is widely viewed as an inflammatory disease with hypercholesterolemia being a dominant underlying risk factor. This study aimed to determine the effect of mangosteen pericarp in inhibition of NF-?B activation and ICAM-1 expression in rat fed with high cholesterol.Methods and Results: Various doses of crude extract mangosteen pericarp were administered to the high fat diet wistar rats and the activity of NF-?B measured by immunohistochemistry to assess nuclear NF-?B expression and the ICAM-1 expression. The high fat diet resulted significant increased serum LDL levels. Increased nuclear NF- ?B activation and ICAM-1 expression were also observed in high fat diet rats in concurrence with increased serum LDL. The inhibitory effect on NF- ?B activity and ICAM-1 expression was observed when 400 mg of mangosteen pericarp crude extract was administered and even showed a higher inhibitory effect in 800 mg of mangosteen pericap treated rats. The 800 mg extract treatment resulted in decreased ICAM-1 expression similar to those of non high fat rats.Conclusion: The administration of 800 mg mangosteen pericarp crude extract significantly inhibited NF-?B activation and reversed the expression of ICAM -1 to the normal level in high cholesterol diet rats.

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The Comparison of Bay Leaf and Celery Leaf Infusion Effect on Decreasing LDL Level in Dyslipidemic Wistar Rats Model

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v1i1.1700 ◽

1970 ◽

Vol 1 (1) ◽

pp. 21

Author(s):

Anjani Putri Retnaninggalih ◽

Erfan Efendi ◽

Hairrudin Hairrudin

Keyword(s):

Cholesterol Level ◽

High Fat Diet ◽

Wistar Rats ◽

Ldl Cholesterol ◽

Wistar Rat ◽

Positive Control ◽

Negative Control ◽

High Fat ◽

Leaf P ◽

One Way Anova

Coronary heart disease (CHD) is the cause of 7,3 million death globally in 2008. The main pathophysiology process that leads to CHD is atherosclerosis which is caused by high LDL cholesterol level. The aim of this study was to explore bay leaf and celery leaf infusion effect on reducing LDL cholesterol level in dyslipidemic rat model. The type of this study was true experimental research using wistar rat (Rattus novergicus) as the sample. The rats were induced by high fat diet and PTU for two weeks then each of the group were given either bay leaf infusion, celery leaf infusion, simvastatin (positive control), or aquadest (negative control) for a week. The measurement of LDL level used Friedewald formulation. Data was analyzed using paired sample t test and one way ANOVA. As the result there was a significance reduction on LDL level after treatment with bay leaf (p=0,013) and celery leaf infusion (p=0,035) but there was no significance difference between groups (p=0,293). It was concluded that bay leaf and celery leaf infusion could reduce the LDL level in dyslipidemic wistar rat but there was no difference on the capability of the two different leafs on reducing LDL level. Keywords: dyslipidemia, LDL, bay leaf, celery leaf, wistar rats

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