A simple polymerase chain reaction technique to detect and differentiate Shigella and enteroinvasive escherichia coli in human feces

1997 ◽  
Vol 28 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Huo-Shu H. Houng ◽  
Orntipa Sethabutr ◽  
Peter Echeverria
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Technique ◽  
Human Feces ◽  
Chain Reaction ◽  
Polymerase Chain

Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

2019 ◽  
Vol 19 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Hassan Valadbeigi ◽  
Elham Esmaeeli ◽  
Sobhan Ghafourian ◽  
Abbas Maleki ◽  
Nourkhoda Sadeghifard
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multiplex Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Uropathogenic Escherichia Coli ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

The effect of gold nanoparticles on the diagnostic polymerase chain reaction technique for equine herpes virus 1 (EHV-1)

RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54898-54903 ◽  
Author(s):  
Dalia M. El-Husseini ◽  
Nashwa M. Helmy ◽  
Reham H. Tammam
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Herpes Virus ◽  
Polymerase Chain Reaction Technique ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

We experimented the effect of 15 nm unmodified citrate coated GNPs on the key PCR reactants to see if these would enhance the overall outcomes of the reaction. Thus, the optimized GNPs-assisted PCR could be used for more efficient diagnosis of EHV-1.

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