scholarly journals Isolation of Escherichia coli O157:H7 in Poultry by Culture, Serology and Polymerase Chain Reaction Technique in Jos, North Central Nigeria

2019 ◽  
Vol 40 (1) ◽  
pp. 86
Author(s):  
O.H. Buharshak ◽  
D.Z. Egah ◽  
K. Onyedibe
2019 ◽  
Vol 19 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Hassan Valadbeigi ◽  
Elham Esmaeeli ◽  
Sobhan Ghafourian ◽  
Abbas Maleki ◽  
Nourkhoda Sadeghifard

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.


RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54898-54903 ◽  
Author(s):  
Dalia M. El-Husseini ◽  
Nashwa M. Helmy ◽  
Reham H. Tammam

We experimented the effect of 15 nm unmodified citrate coated GNPs on the key PCR reactants to see if these would enhance the overall outcomes of the reaction. Thus, the optimized GNPs-assisted PCR could be used for more efficient diagnosis of EHV-1.


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