5605812 Test kit and method for the quantitative determination of coliform bacteria and E. coli

1997 ◽  
Vol 15 (3-4) ◽  
pp. 746
Keyword(s):  
Quantitative Determination ◽  
Coliform Bacteria ◽  
E Coli ◽  
Test Kit

scholarly journals Could the miraculous springs in the Pardubice region be used as drinking water sources?

2012 ◽  
Vol 29 (Special Issue) ◽  
pp. S109-S114
Author(s):  
M. Pejchalová ◽  
P. Hanzalová ◽  
R. Metelka ◽  
J. Vytřasová
Keyword(s):  
Membrane Filtration ◽  
Potable Water ◽  
Coliform Bacteria ◽  
E Coli ◽  
Quantification Method ◽  
Alternative Cultivation ◽  
Microbiological Analyses ◽  
Filtration Technique ◽  
Intestinal Enterococci

Selected springs in the Pardubice Region were repeatedly examined for analytical and microbiological indicators. Microbiological analyses included the determination of intestinal enterococci, coliform bacteria, E. coli, and the number of colonies cultivated at temperature form 22&deg;C to 36&deg;C. The analyses were carried out according to the relevant CSN ISO norms using the membrane filtration technique and also the alternative cultivation method Colilert<sup>&reg;</sup>/Quanti-Tray 2000<sup>&reg;</sup> quantification method using the defined substrate technology. Out of 11 wells and springs examined, only two satisfied the requirements for potable water with all indicators observed.

COLIFORM BACTERIA IN SEA WATER AND SHELLFISH: II. THE E. C. CONFIRMATION TEST FOR ESCHERICHIA COLI

1961 ◽  
Vol 7 (5) ◽  
pp. 733-739 ◽  
Author(s):  
A. D. Tennant ◽  
J. E. Reid ◽  
L. J. Rockwell ◽  
E. T. Bynoe
Keyword(s):  
Escherichia Coli ◽  
Sea Water ◽  
The United States ◽  
Coliform Bacteria ◽  
Simple Test ◽  
E Coli ◽  
Confirmation Test ◽  
The Mean ◽  
Test Cultures

Recently there has been considerable interest in Canada and the United States in the development of an effective, simple test for the estimation of Escherichia coli densities in shellfish and shellfish-growing waters as a better indication of "faecal" pollution than that provided by the coliform group. The fidelity of the E.C. confirmation test for the determination of E. coli densities was evaluated in 15 shellfish-growing areas which were classified as "polluted" or "unpolluted" according to generally accepted criteria. Coliform strains were isolated from 2765 E.C. gas positive confirmation test cultures and identified by IMViC tests. The mean fidelity of the test, as an indication of the presence of E. coli, was circa 90 per cent; contrary to expectations, however, the per cent recovery of E. coli was higher in unpolluted areas than in polluted areas, and varied considerably from region to region. Aerobacter aerogenes types I and II were the most common other E.C. gas positive coliform biotypes found. The usefulness of the confirmation test and the sanitary significance of these data are discussed.

scholarly journals Quantitative and Qualitative Composition of Bacterial Communities of Malting Barley Grain and Malt during Long-Term Storage

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1301
Author(s):  
Soňa Felšöciová ◽  
Przemysław Łukasz Kowalczewski ◽  
Tomáš Krajčovič ◽  
Štefan Dráb ◽  
Miroslava Kačániová
Keyword(s):  
Quantitative Determination ◽  
Storage Period ◽  
Barley Grain ◽  
Coliform Bacteria ◽  
Dilution Method ◽  
Malting Barley ◽  
Long Term Storage ◽  
Plate Counts ◽  
Barley Grains

In the grain samples of three malting barley varieties harvested in 2016, and in the malt made from them, we observed microbial changes during storage. Analyses were performed after 0, 3, 6, and 9 months of storage in floor warehouses and silos. We monitored microbial changes based on the quantitative determination of microorganisms using a plate dilution method and qualitative determination of microorganisms, where we identified a particular species of bacteria using the MALDI-TOF MS Biotyper instrument (Bruker Daltonics, Bremen, Germany). Based on the quantitative determination of microorganisms, we found that the total plate counts of microorganisms was higher in malts than in barley grains. The storage period had the greatest impact on the number of coliform bacteria, which gradually increased with the growing months in storage. The number of lactic acid bacteria in barley grains with the growing months in storage and malt produced from them decreased slightly, and the content of the sporulating bacteria in all the samples fluctuated slightly. Differences in the numbers of observed microorganisms were negligible during storage between floor warehouses and silos. In the samples of barleys, the most commonly identified species were Pantoea agglomerans, which was also present in malt samples but did not belong to the dominant species. A diverse species representation appeared in the samples, whereas predominating species belonged to the Enterobacteriaceae family.

Quantitative determination of Escherichia coli in water using CHROMagar® E. coli

1996 ◽  
Vol 25 (3) ◽  
pp. 309-315 ◽  
Author(s):  
Jose L. Alonso ◽  
Inmaculada Amoros ◽  
Steven Chong ◽  
Hemda Garelick
Keyword(s):  
Escherichia Coli ◽  
Quantitative Determination ◽  
E Coli

Enzymatic Determination of Cholesterol in Egg Yolk

1982 ◽  
Vol 65 (5) ◽  
pp. 1222-1224 ◽  
Author(s):  
Chih-Shang J Shen ◽  
Isabel S Chen ◽  
Alan J Sheppard
Keyword(s):  
Quantitative Determination ◽  
Methylene Chloride ◽  
Egg Yolk ◽  
Cholesterol Content ◽  
Enzymatic Determination ◽  
Test Kit ◽  
The Mean ◽  
Liquid Chromatographic ◽  
Enzymatic Test

Abstract The quantitative determination of cholesterol in egg yolk by using an enzymatic test kit is described. Cholesterol in the egg yolk is extracted with other lipid components by methylene chloride-methanol (2 + 1) and is enzymatically determined after saponification of the lipid extract. The method is relatively rapid, simple, and accurate and gives results which agree with those obtained by using a gas-liquid chromatographic (GLC) method. The mean cholesterol content of egg yolk determined by the enzymatic and GLC methods was 1237 and 1240 mg/100 g, respectively.

scholarly journals Development of Method for Quantitative Determination of Water Arsenic by Field Test Kit

2018 ◽  
Vol 3 (1) ◽  
pp. 340 ◽  
Author(s):  
Makoto SEKINE ◽  
Masahiro TOKUMURA ◽  
Mohammad RAKNUZZAMAN ◽  
Md AHMED ◽  
Md Islam ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Field Test ◽  
Test Kit

scholarly journals Occurence of Mastitis in Dairy Cows Situated in Marginal Parts of Slovakia

2017 ◽  
Vol 61 (4) ◽  
pp. 59-64
Author(s):  
F. Zigo ◽  
M. Vasiľ ◽  
J. Elečko ◽  
Z. Farkašová ◽  
M. Zigová
Keyword(s):  
Dairy Cows ◽  
Enterobacter Aerogenes ◽  
Subclinical Mastitis ◽  
Coliform Bacteria ◽  
Economic Stability ◽  
Coagulase Negative Staphylococci ◽  
E Coli ◽  
California Mastitis Test ◽  
Streptococcus Uberis

AbstractA relatively large part of the Slovak territory consists of the “marginal regions”, which in terms of the economy of ruminants keeping can efficiently produce animal commodities only occasionally. The geographic, social and economic stability of these regions is strongly influenced by the rearing of ruminants and the associated market milk production. The aim of this study was to evaluate the prevalence and aetiological agents of mastitis in two herds of dairy cows situated in the marginal parts of Slovakia. In total, 530 of the Slovak spotted breed and Holstein cows were involved in the study. The diagnosis of mastitis was performed on the basis of the clinical examination of the udder, macroscopic evaluation of the milk, determination of somatic cell count (SCC), and the bacteriological examination of the milk. The prevalence of mastitis in the two herds of dairy cows ranged from 34.7 % to 18.8 %, respectively. From the total of 2120 quarter milk samples, 36.3 % were positive to the California mastitis test (CMT). Also, pathogenic microorganisms causing intramammary infection (IMI) were isolated from 25.6 % of the samples, which accounted for most subclinical mastitis forms (23.3 %), with the SCC under 400 000, mainly caused by coagulase-negative staphylococci (CoNS) and coliform bacteria E. coli and Enterobacter aerogenes. The clinical forms of mastitis accounted for 13.0 % of all infected cows and were caused mainly by the bacteria: Streptococcus uberis, Streptococcus agalactiae, Staphylococcus aureus and coagulasenegative staphylococci.

scholarly journals Microbiological Profile of Kashkaval Cheese During Production Season

2020 ◽  
Vol 77 (1) ◽  
pp. 91
Author(s):  
Mirela Anamaria JIMBOREAN ◽  
Dorin ŢIBULCĂ ◽  
Delia MICHIU
Keyword(s):  
Starter Cultures ◽  
Coliform Bacteria ◽  
Suitable Habitat ◽  
E Coli ◽  
Microbiological Profile ◽  
Indigenous Microbiota ◽  
One Year ◽  
Microbiological Assays ◽  
Coagulase Positive Staphylococci

Although the scalded cheeses are not an ideal substrate for microorganisms, these are a suitable habitat for starter cultures and indigenous microbiota, the latter being able to survive during processing. The aim of this study was to highlight the qualitative and hygienic aspects of kashkavals as a finished products. The microbiological assays aimed the evaluation of sanitary-hygienic quality in kashkaval cheeses obtained during one year, by the following indicators: determination of coliform bacteria, E. coli, monitoring of pathogenic microorganisms: Stafylococcus aureus coagulase-positive and total number of micromycetes. Sixty samples from five units which process the milk into scalded cheeses, were collected. 10% from the analyzed cheese samples, had values of coliform counts between 102-103 cfu/g, while the remaining samples had values less than 102 cfu/g. 8.3% of cheese samples had values of E. coli ranging from 11 to 100 cfu/g and the remaining samples had lower values . No sample registered over 1,000 cfu/g, which is the upper marginal limit for coagulase-positive staphylococci in cheeses, according to the Reg. EC 1881/2006. 18.3% of cheese samples had values of total number of micromycetes over 1000 cfu/g, while the remaining samples were below 1000 cfu/ g.

Sign in / Sign up

Export Citation Format

Share Document