Direct Seeded Rice and its Prospects in Nepal: A Review

Direct Seeded Rice (DSR) is an alternative cultivation technique to the conventional Transplanted Rice (TPR). In this method, seeds are directly sown to the field without the necessity of nursery raising and transplanting. DSR can generally be divided into dry-DSR (sowing dry seeds in dry soil), wet-DSR (sowing pre-germinated seeds in moist soil) and water-seeding (sowing dry or pre-germinated seeds in standing water). This helps in saving water alongside the labour and results in early harvest due to quick crop establishment. DSR is proved to be sustainable and eco-friendly since it emits less methane than in TPR. However, there are few constraints associated with DSR such as weed infestation, crop lodging and nutrient loss. If these major issues are fixed, DSR can result in greater economic returns as compared to TPR. In absence of puddling, the soil structure can be maintained which leads to greater yields of succeeding crops. This technique has been successfully practiced in many countries like Srilanka, Malaysia and USA. In developing nations like Nepal, irrigation and labour constraints can be tackled by adopting direct-seeding for rice cultivation.

Environmental impact of tsipouro production by life cycle assessment

The study of the environmental impact of agricultural products has significantly grown in recent years, as consumers now demand more information about the product’s footprint in the environment. The aim of this study is to assess the environmental impact of the life cycle phases of tsipouro production, which is one of the traditional products of Greece produced mainly from viticulture. The environmental analysis was performed through the study of eutrophication, global warming, photochemical oxidation and acidification, using the life cycle assessment methodology. The system was studied through fifteen subsystems and a 250 ml bottle of tsipouro, which was the basis of the calculations, was defined as a functional unit. From the results it appears that the process of tsipouro production is the subsystem with the highest energy consumption and the grape cultivation the one with the highest water consumption. In environmental impact the subsystem with the highest contribution is the cultivation of grapes. Also the subsystems production/transportation and use of fertilizers, bottle production/transportation and the process of tsipouro production have a significant contribution. In addition, some literature-based solutions are suggested. Some of the solutions are the use of clearer energy sources, the use of biodiesel and alternative cultivation methods without synthetic fertilizers. The results of this research can be used by tsipouro or similar industries to minimize the environmental impact and focus on the phases that are most involved in it.

Impact of Using Organic Yeast in the Fermentation Process of Wine

The aim of this study was to find out what kind of “Bianca” wine could be produced when using organic yeast, what are the dynamics of the resulting alcoholic fermentation, and whether this method is suitable for industrial production as well. Due to the stricter rules and regulations, as well as the limited amount and selection of the permitted chemicals, resistant, also known as interspecific or innovative grape varieties, can be the ideal basic materials of alternative cultivation technologies. Well-designed analytical and organoleptic results have to provide the scientific background of resistant varieties, as these cultivars and their environmentally friendly cultivation techniques could be the raw materials of the future. The role of the yeast in wine production is crucial. We fermented wines from the “Bianca” juice samples three times where model chemical solutions were applied. In our research, we aimed to find out how organic yeast influenced the biogenic amine formation of three important compounds: histamine, tyramine, and serotonin. The main results of this study showed that all the problematic values (e.g., histamine) were under the critical limit (1 g/L), although the organic samples resulted in a significantly higher level than the control wines. The glycerin content correlated with the literature values, since it is well known that the glycerin-pyruvic acid transformation results in a 6–10 g/L concentration.

EFFECT OF VARIOUS SUBSTRATES IN NON-CIRCULATING HYDROPONIC SYSTEMS AND SOIL MEDIA ON THE GROWTH OF Gynura procumbens

The limitations of conventional soil cultivation in producing medicinal plants emerge an interest in searching various alternative cultivation systems, one of which is the hydroponic system. This study aimed to investigate the effect of various substrates in non-circulating hydroponic systems and soil media on the growth of Gynura procumbens. Non-circulating hydroponic cultivation of Gynura procumbens was carried out using stem cuttings. The stem cuttings were transferred to each net pot containing substrates such as rockwool, tree ferns fiber, and sawdust. The net pots were then placed in a tray comprising AB mix 6 ml/L of water. The lower 2-cm-portion of the net pots was immersed in a nutrient solution. Whereas in the conventional soil cultivation, the stem cuttings were transferred to the polybags containing soil media: compost (1: 1). After 28 days of cultivation, the plant growths were measured. The results showed that the use of various substrates in non-circulating hydroponics increased plant growth compared to conventional soil cultivation. Rockwool treatment obtained the highest number of leaves (14,833 ± 5,269 strands), number of roots (20,333 ± 6,121 stands), fresh weight (1.34 ± 0.511 g) and dry weight (0.088 ± 0.031 g).

Untargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion

Cyanobacteria play an important role in several ecological environments, and they are widely accepted to be the ancestors of chloroplasts in modern plants and green algae. Cyanobacteria have become attractive models for metabolic engineering, with the goal of exploring them as microbial cell factories. However, the study of cyanobacterial lipids’ composition and variation, and the assessment of the lipids’ functional and structural roles have been largely overlooked. Here, we aimed at expanding the cyanobacterial lipidomic analytical pipeline by using an untargeted lipidomics approach. Thus, the lipid composition variation of the model cyanobacterium Synechocystis sp. PCC 6803 was investigated in response to both alternative cultivation setups and gene deletion. This approach allowed for detecting differences in total lipid content, alterations in fatty-acid unsaturation level, and adjustments of specific lipid species among the identified lipid classes. The employed method also revealed that the cultivation setup tested in this work induced a deeper alteration of the cyanobacterial cell lipidome than the deletion of a gene that results in a dramatic increase in the release of lipid-rich outer membrane vesicles. This study further highlights how growth conditions must be carefully selected when cyanobacteria are to be engineered and/or scaled-up for lipid or fatty acids production.

Arbuscular Mycorrhizal Fungi Modulate the Crop Performance and Metabolic Profile of Saffron in Soilless Cultivation

Saffron (Crocus sativus L.) is cultivated worldwide. Its stigmas represent the highest-priced spice and contain bioactive compounds beneficial for human health. Saffron cultivation commonly occurs in open field, and spice yield can vary greatly, from 0.15 to 1.5 g m−2, based on several agronomic and climatic factors. In this study, we evaluated saffron cultivation in soilless systems, where plants can benefit from a wealth of nutrients without competition with pathogens or stresses related to nutrient-soil interaction. In addition, as plant nutrient and water uptake can be enhanced by the symbiosis with arbuscular mycorrhizal fungi (AMF), we also tested two inocula: a single species (Rhizophagus intraradices) or a mixture of R. intraradices and Funneliformis mosseae. After one cultivation cycle, we evaluated the spice yield, quality (ISO category), antioxidant activity, and bioactive compound contents of saffron produced in soilless systems and the effect of the applied AMF inocula. Spice yield in soilless systems (0.55 g m−2) was on average with that produced in open field, while presented a superior content of several health-promoting compounds, such as polyphenols, anthocyanins, vitamin C, and elevated antioxidant activity. The AMF symbiosis with saffron roots was verified by light and transmission electron microscopy. Inoculated corms showed larger replacement corms (+50% ca.). Corms inoculated with R. intraradices performed better than those inoculated with the mix in terms of spice quality (+90% ca.) and antioxidant activity (+88% ca.). Conversely, the mixture of R. intraradices and F. mosseae increased the polyphenol content (+343% ca.). Thus, soilless systems appeared as an effective alternative cultivation strategy for the production of high quality saffron. Further benefits can be obtained by the application of targeted AMF-based biostimulants.