Effects of brevianamide A, its photolysis product brevianamide D, and ochratoxin A from two Penicillium strains on the insect pests Spodoptera frugiperda and Heliothis virescens

Essential oils of Piper subtomentosum (leaves and inflorescences) and Piper septuplinervium (aerial parts) were analyzed by GC-MS; sixty-three compounds were determined, representing 92.0%, 86.9 %, and 91.8 % of the total relative oil composition of the leaves, inflorescences, and aerial parts, respectively. The most abundant component in the aerial parts and inflorescence oils was α-pinene (27.3%, 21.0%, respectively), and δ-cadinene was the main component of the leaf oil. Insecticidal activity of the essential oils were determined on the Spodoptera frugiperda second instar larvae; the essential oil from the aerial parts of P. septuplinervium was the most active against insect pests (LC50= 9.4 μL/L of air). Statistical analysis by direct Pearson correlation showed that the insecticidal activity of the essential oils was primarily due to camphene and α- and β-pinene. The effect of the oils on the insect life cycle was also evaluated, and in some cases, a delay in growth and inhibition of the oviposition in the females were observed.

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Specific Binding of Radiolabeled Cry1Fa Insecticidal Protein from Bacillus thuringiensis to Midgut Sites in Lepidopteran Species

Applied and Environmental Microbiology ◽

10.1128/aem.07591-11 ◽

2012 ◽

Vol 78 (11) ◽

pp. 4048-4050 ◽

Cited By ~ 9

Author(s):

Carmen Sara Hernández-Rodríguez ◽

Patricia Hernández-Martínez ◽

Jeroen Van Rie ◽

Baltasar Escriche ◽

Juan Ferré

Keyword(s):

Spodoptera Frugiperda ◽

Heliothis Virescens ◽

Brush Border Membrane ◽

Specific Binding ◽

Membrane Vesicles ◽

Insecticidal Protein ◽

Content Type ◽

Lepidopteran Species ◽

Equilibrium Binding ◽

Competition Assays

ABSTRACTCry1Fa insecticidal protein was successfully radiolabeled with125I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran speciesOstrinia nubilalis,Spodoptera frugiperda,Spodoptera exigua,Helicoverpa armigera,Heliothis virescens, andPlutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (Kd[dissociation constant] andRt[concentration of binding sites]) for these six insect species.

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Relação entre toxicidade de proteínas Vip3Aa e sua capacidade de ligação a receptores intestinais de lepidópteros-praga

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2015000800002 ◽

2015 ◽

Vol 50 (8) ◽

pp. 637-648 ◽

Cited By ~ 2

Author(s):

Suzana Cristina Marucci ◽

Camila Soares Figueiredo ◽

Renata Izabel Dozzi Tezza ◽

Eliane Cristina da Cunha Alves ◽

Manoel Victor Franco Lemos ◽

...

Keyword(s):

Spodoptera Frugiperda ◽

Heliothis Virescens

Resumo:O objetivo deste trabalho foi avaliar a toxicidade de novas proteínas Vip3Aa e sua capacidade de ligação a vesículas de membrana da microvilosidade apical (VMMA) do intestino de lagartas neonatas de Spodoptera frugiperda, Anticarsia gemmatalise Heliothis virescens. Proteínas expressas pelos genes vip3Aa42 e vip3Aa43 mostraram-se tóxicas a S. frugiperda (CL50 de 78,2 e 113 ng cm-2, respectivamente) e A. gemmatalis(CL50 de 239,2 e 57,5 ng cm-2, respectivamente), e pouco tóxicas a H. virescens (CL50>5.000 ng cm-2). Os ensaios de ligação às VMMA mostraram que as proteínas unem-se de forma efetiva aos receptores nas vesículas das espécies avaliadas, mas essa capacidade de ligação somente é efetiva na ativação da toxicidade para as populações avaliadas de S. frugiperdae A. gemmatalis.

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Toxicity of Naturally Occurring Levels of the Penicillium Mycotoxins Citrinin, Ochratoxin A, and Penicillic Acid to the Corn Earworm, Heliothis zea , and the Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)

Environmental Entomology ◽

10.1093/ee/18.1.24 ◽

1989 ◽

Vol 18 (1) ◽

pp. 24-29 ◽

Cited By ~ 13

Author(s):

P. F. Dowd

Keyword(s):

Ochratoxin A ◽

Spodoptera Frugiperda ◽

Heliothis Zea ◽

Fall Armyworm ◽

Corn Earworm ◽

Penicillic Acid ◽

Naturally Occurring ◽

Lepidoptera Noctuidae

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Bacillus thuringiensis Vip3Aa Toxin Resistance in Heliothis virescens (Lepidoptera: Noctuidae)

Applied and Environmental Microbiology ◽

10.1128/aem.03506-16 ◽

2017 ◽

Vol 83 (9) ◽

Cited By ~ 22

Author(s):

Brian R. Pickett ◽

Asim Gulzar ◽

Juan Ferré ◽

Denis J. Wright

Keyword(s):

Bacillus Thuringiensis ◽

Heliothis Virescens ◽

Insect Pests ◽

Management Strategies ◽

Resistance Management ◽

Cross Resistance ◽

Inheritance Of Resistance ◽

Resistant Parent ◽

Bt Crops ◽

Content Type

ABSTRACT Laboratory selection with Vip3Aa of a field-derived population of Heliothis virescens produced >2,040-fold resistance in 12 generations of selection. The Vip3Aa-selected (Vip-Sel)-resistant population showed little cross-resistance to Cry1Ab and no cross-resistance to Cry1Ac. Resistance was unstable after 15 generations without exposure to the toxin. F1 reciprocal crosses between Vip3Aa-unselected (Vip-Unsel) and Vip-Sel insects indicated a strong paternal influence on the inheritance of resistance. Resistance ranged from almost completely recessive (mean degree of dominance [h] = 0.04 if the resistant parent was female) to incompletely dominant (mean h = 0.53 if the resistant parent was male). Results from bioassays on the offspring from backcrosses of the F1 progeny with Vip-Sel insects indicated that resistance was due to more than one locus. The results described in this article provide useful information for the insecticide resistance management strategies designed to overcome the evolution of resistance to Vip3Aa in insect pests. IMPORTANCE Heliothis virescens is an important pest that has the ability to feed on many plant species. The extensive use of Bacillus thuringiensis (Bt) crops or spray has already led to the evolution of insect resistance in the field for some species of Lepidoptera and Coleoptera. The development of resistance in insect pests is the main threat to Bt crops. The effective resistance management strategies are very important to prolong the life of Bt plants. Lab selection is the key step to test the assumption and predictions of management strategies prior to field evaluation. Resistant insects offer useful information to determine the inheritance of resistance and the frequency of resistance alleles and to study the mechanism of resistance to insecticides.

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Effect of seed treatment with insecticides on the control of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in soybean

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000332018 ◽

2019 ◽

Vol 86 ◽

Cited By ~ 1

Author(s):

Yago Barros Triboni ◽

Luciano Del Bem Junior ◽

Carlos Gilberto Raetano ◽

Matheus Mereb Negrisoli

Keyword(s):

Spodoptera Frugiperda ◽

Seed Treatment ◽

Yield Loss ◽

Insect Pests ◽

Fall Armyworm ◽

Control Treatment ◽

Control Efficacy ◽

Thiophanate Methyl ◽

Crop Development ◽

Lepidoptera Noctuidae

ABSTRACT Fall armyworm is one of the main insect pests in Brazil. Thus, the present work aimed to evaluate the seed treatment effect on the control of Spodoptera frugiperda in initial infestations of soybean crops. The experimental design was completely randomized with four replicates of six insecticide treatments applied through seed treatment: imidacloprid plus thiodicarb at the dose of 52.5 plus 105 g a.i. (active ingredient) 100 kg-1 of seed (Cropstar® 0.350 L 100 kg-1 of seed); thiamethoxam at 105 g a.i. 100 kg-1 of seed (Cruiser 350 FS® 0.3 L 100 kg-1 of seed); chlorantraniliprole at 62.5 g a.i. 100 kg-1 of seed (Dermacor® 0.1 L 100 kg-1 of seed); cyantraniliprole at 120 g a.i. 100 kg-1 of seed (Fortenza 600 FS® 0.2 L 100 kg-1 of seed); fipronil plus pyraclostrobin and thiophanate-methyl 50 + 5 + 45 g a.i. 100 kg-1 of seed (Standak Top® 0.2 L 100 kg-1 of seed), and a control treatment. The experiment was carried out in a greenhouse. Diamide insecticides (chlorantraniliprole and cyantraniliprole) presented the best results among all treatments, with lower consumption of the treated leaves by the caterpillars and greater control efficacy of this insect. We verified that seed treatment is a viable alternative for controlling S. frugiperda at the beginning of crop development, when the caterpillar presents the behavior of cutting the seedlings and/or the consumption of leaf area, causing a reduction in the plant population and a consequent yield loss.

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Water in maize whorl enhances the control of Spodoptera frugiperda with insecticides

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632020v5059517 ◽

2020 ◽

Vol 50 ◽

Author(s):

Adriano Bialozor ◽

Clérison Régis Perini ◽

Jonas André Arnemann ◽

Henrique Pozebon ◽

Adriano Arrué Melo ◽

...

Keyword(s):

Grain Yield ◽

Spodoptera Frugiperda ◽

Insect Pests ◽

Fall Armyworm ◽

Early Growth ◽

Growth Stages ◽

Bt Maize ◽

Maize Hybrids ◽

Cropping Seasons ◽

And Control

ABSTRACT The fall armyworm Spodoptera frugiperda is among the main insect-pests on maize crops, due to its damaging potential and control issues related to the larval habit of concealing itself within the plant whorl. This study aimed to evaluate the effect of water in the maize whorl, combined with insecticides and spray sets, on the damage caused by S. frugiperda and the grain yield. The experiments were carried out under field conditions, at two cropping seasons, both with Bt-maize hybrids, in a 2 × 2 × 5 + 1 factorial scheme, testing the presence or absence of water inside the plant whorl, two insecticides and five spray sets (combinations of spray volumes, nozzle types, pressures and spraying speed), plus a control without water and insecticide. The presence of water inside the whorl reduced the damage caused by S. frugiperda during the early growth stages, especially in the plants sprayed with the insecticide chlorantraniliprole. The spray sets with 200 L ha1/20.3 psi or 250 L ha1/33.4 psi provided the highest reductions in the percentage of damaged plants and damage scores, regardless of the insecticide. Therefore, the spraying of insecticides, when there is water from irrigation, dew or rainfall inside the maize whorl, improves the control of S. frugiperda in maize crops, especially in Bt-maize.

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Evaluation of Piper marginatum (Piperales: Piperaceae) Oil and Geraniol on the Embryonic Development of Spodoptera frugiperda (Lepidoptera: Noctuidae) in Comparison to Formulated Products

Journal of Economic Entomology ◽

10.1093/jee/toz300 ◽

2020 ◽

Vol 113 (1) ◽

pp. 239-248 ◽

Cited By ~ 1

Author(s):

Carolina A Guedes ◽

Valeria Wanderley Teixeira ◽

Kamila A Dutra ◽

Daniela M A F Navarro ◽

Glaucilane S Cruz ◽

...

Keyword(s):

Embryonic Development ◽

Spodoptera Frugiperda ◽

Insect Pests ◽

Negative Control ◽

Botanical Insecticide ◽

Synthetic Insecticide ◽

Significant Damage ◽

Semithin Sections ◽

Lepidoptera Noctuidae ◽

Scanning Electron

Abstract Essential oils and their isolated constituents are constantly being studied for the control of insect pests. In this context, the present research reports the chemical composition of Piper marginatum (Jacq.) oil aiming to: 1) establish lethal concentrations LC30 and LC50 for this oil and the compound geraniol, 2) histologically examine the embryonic development of Spodoptera frugiperda (J.E. Smith) through light microscopy and scanning electron microscopy (SEM), as well as 3) compare the efficacy of the P. marginatum oil with that of the botanical insecticide azadirachtin, the synthetic insecticide deltamethrin, and acetone as a negative control. Semithin sections of S. frugiperda eggs revealed that the oil, geraniol, azadirachtin, and deltamethrin affected embryonic development at both concentrations. However, geraniol and the oil were more efficient because they caused more significant damage, even at lower concentrations. SEM revealed that all products altered the morphology of the eggs, modifying the structure of the chorion and making the eggs nonviable. Thus, this work demonstrates that P. marginatum oil is effective in the control of S. frugiperda because it results in embryonic damage even at the lowest concentrations.

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SYNERGISM OF PYRETHROIDS BY PIPERONYL BUTOXIDE AND MGK-264 AGAINST HELIOTHIS VIRESCENS, SPODOPTERA EXIGUA, AND SPODOPTERA FRUGIPERDA (LEPIDOPTERA: NOCTUIDAE)1

Journal of Entomological Science ◽

10.18474/0749-8004-23.3.229 ◽

1988 ◽

Vol 23 (3) ◽

pp. 229-233

Author(s):

J. A. Joyce ◽

R. J. Ottens ◽

G. A. Herzog ◽

M. H. Bass

Keyword(s):

Spodoptera Frugiperda ◽

Heliothis Virescens ◽

Spodoptera Exigua ◽

Synergistic Effects ◽

Fall Armyworm ◽

Piperonyl Butoxide ◽

Tobacco Budworm ◽

Lepidoptera Noctuidae ◽

Beet Army Worm ◽

Army Worm

Laboratory cultures of field-collected larval tobacco budworm, Heliothis virescens (F.), beet army worm, Spodoptera exigua (Hübner), and fall armyworm, S. frugiperda (J. E. Smith) were bioassayed for response to three pyrethroids in combination with piperonyl butoxide (PBO), or MGK-264. The greatest synergistic effects were seen in S. exigua which also displayed the greatest tolerance to pyrethroids without synergists. The highest SR50 (synergist ratio) value for S. exigua was 22.1 with fenvalerate-PBO mixed in a ratio of 1:5, the highest for S. frugiperda was 4.6 with fenvalerate-MGK-264 1:5, and the highest for H. virescens was 1.3 with permethrin-MGK-264 1:5 or with fenvalerate-PBO 1:5.

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