8712 POSTER Validation of Differential mRNA Expression of Genes in Astrocytoma

2011 ◽  
Vol 47 ◽  
pp. S578
Author(s):  
C. Diez-Tascón ◽  
A. de la Hera ◽  
O. Rivero-Lezcano ◽  
L. Vilorio ◽  
E. Santin ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Expression Of Genes ◽  
Differential Mrna Expression

scholarly journals Differential mRNA expression of genes in the porcine adrenal gland associated with psychosocial stress

2011 ◽  
Vol 46 (3) ◽  
pp. 165-174 ◽  
Author(s):  
E. Murani ◽  
S. Ponsuksili ◽  
R. B. D'Eath ◽  
S. P. Turner ◽  
G. Evans ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Mrna Expression ◽  
Psychosocial Stress ◽  
Expression Of Genes ◽  
Differential Mrna Expression

Abstract 191: NF-?B p65 Methylation by Protein Arginine Methyltransferase 5 (PRMT5) is Necessary for the Transcriptional Induction of CXCL10 by TNF-a

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Daniel P Harris
Keyword(s):  
Endothelial Cells ◽  
Mrna Expression ◽  
Chromatin Immunoprecipitation ◽  
Proximal Promoter ◽  
Protein Arginine Methyltransferase ◽  
Arginine Methyltransferase ◽  
Expression Of Genes ◽  
Tnf Α ◽  
Affect Expression ◽  
Transcriptional Induction

TNF-α initiates the expression of genes involved in the recruitment, adhesion, and transmigration of leukocytes to sites of inflammation. Here, we report that the protein arginine methyltransferase PRMT5 is required for the transcriptional induction of the pro-inflammatory chemokine CXCL10 (IP-10) in endothelial cells. Depletion of PRMT5 by siRNA results in significantly diminished TNF-α-induced CXCL10 mRNA expression, but does not affect expression of other chemokines, such as MCP-1 or IL-8. Chromatin immunoprecipitation experiments of the CXCL10 proximal promoter show the presence of symmetrical dimethylated arginine (sDMA)-containing proteins upon exposure to TNF-α. This methylation is completely lost when PRMT5 is removed from cells by siRNA. Using immunoprecipitation, we show that PRMT5 enhances CXCL10 expression by methylating the RelA (p65) subunit of NF-κB. In summary, we have identified that PRMT5 is a novel regulator of CXCL10 expression. Further, we have discovered that PRMT5 methylates NF-κB, a finding which may further knowledge of the post-translational code governing NF-κB regulation and target specificity.

Effects of lipotoxicity on a novel insulin-secreting human pancreatic β-cell line, 1.1B4

2013 ◽  
Vol 394 (7) ◽  
pp. 909-918 ◽  
Author(s):  
Srividya Vasu ◽  
Neville H. McClenaghan ◽  
Jane T. McCluskey ◽  
Peter R. Flatt
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Molecular Mechanisms ◽  
Endoplasmic Reticulum Stress Response ◽  
Future Research ◽  
The Novel ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
Expression Of Genes ◽  
Cellular Ca

Abstract The novel insulin-secreting human pancreatic β-cell line, 1.1B4, demonstrates stability in culture and many of the secretory functional attributes of human pancreatic β-cells. This study investigated the cellular responses of 1.1B4 cells to lipotoxicity. Chronic 18-h exposure of 1.1B4 cells to 0.5 mm palmitate resulted in decreased cell viability and insulin content. Secretory responses to classical insulinotropic agents and cellular Ca2+ handling were also impaired. Palmitate decreased glucokinase activity and mRNA expression of genes involved in secretory function but up-regulated mRNA expression of HSPA5, EIF2A, and EIF2AK3, implicating activation of the endoplasmic reticulum stress response. Palmitate also induced DNA damage and apoptosis of 1.1B4 cells. These responses were accompanied by increased gene expression of the antioxidant enzymes SOD1, SOD2, CAT and GPX1. This study details molecular mechanisms underlying lipotoxicity in 1.1B4 cells and indicates the potential value of the novel β-cell line for future research.

Differential mRNA expression of the avian-specific toll-like receptor 15 between heterophils from Salmonella-susceptible and -resistant chickens

2008 ◽  
Vol 61 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Jessica R. Nerren ◽  
Christina L. Swaggerty ◽  
Kathryn M. MacKinnon ◽  
Kenneth J. Genovese ◽  
Haiqi He ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Toll Like Receptor ◽  
Differential Mrna Expression

scholarly journals Differential mRNA expression in circulating γδ T lymphocyte subsets defines unique tissue-specific functions

2003 ◽  
Vol 73 (2) ◽  
pp. 306-314 ◽  
Author(s):  
Jodi F. Hedges ◽  
Diane Cockrell ◽  
Larissa Jackiw ◽  
Nicole Meissner ◽  
Mark A. Jutila
Keyword(s):  
Mrna Expression ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
T Lymphocyte Subsets ◽  
Tissue Specific ◽  
Differential Mrna Expression

Intraabdominal adhesion formation is associated with differential mRNA expression of metabolic genes PDHb and SDHa

2012 ◽  
Vol 286 (3) ◽  
pp. 683-686 ◽  
Author(s):  
Markus Wallwiener ◽  
Christian W. Wallwiener ◽  
Roger Molinas ◽  
Taufiek K. Rajab ◽  
Sara Y. Brucker ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Adhesion Formation ◽  
Metabolic Genes ◽  
Intraabdominal Adhesion ◽  
Differential Mrna Expression

scholarly journals Short communication: Colostrum versus formula: Effects on mRNA expression of genes related to branched-chain amino acid metabolism in neonatal dairy calves

2020 ◽  
Vol 103 (10) ◽  
pp. 9656-9666 ◽  
Author(s):  
Morteza H. Ghaffari ◽  
Hassan Sadri ◽  
Harald M. Hammon ◽  
Julia Steinhoff-Wagner ◽  
Nico Henschel ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mrna Expression ◽  
Amino Acid Metabolism ◽  
Short Communication ◽  
Acid Metabolism ◽  
Dairy Calves ◽  
Branched Chain Amino Acid ◽  
Expression Of Genes ◽  
Branched Chain
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