Nanospike surface-modified bionic porous titanium implant and in vitro osteogenic performance

Hydroxyapatite (Ca10(PO4)6(OH)2, HAp), carbonated HAp and titanium oxide are of interest for bone-interfacing implant applications, because of their demonstrated osteoconductive properties. They were coated on the titanium implants and investigated the in vitro and in vivo performance. HAp coatings were performed by the thermal substrate method in aqueous solutions. Titanium oxide film was also formed on the titanium implants by gaseous oxidation, or by anodizing in the acidic solution. All the specimens covered with HAp, carbonated HAp or TiO2 (rutile or anatase). were characterized by XRD, EDX, FT-IR and SEM. In the in vitro testing, the mouse osteoblast-like cells (MC3T3-E1) were cultured on the coated and non-coated specimens for up to 30 days. Moreover, the osseointegration was evaluated from the rod specimens implanted in rats femoral for up to 8 weeks. In in vivo evaluations two weeks postimplantation, new bone formed on the coated and non-coated titanium rods in the cancellous bone and cortical bone, respectively. Bone-implant contact ratio, in order to evaluate of new bone formation, was significantly depended on the compound formed on the titanium implant.

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Fabrication and Bioactivity of Porous Titanium Implant

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.613 ◽

2007 ◽

Vol 342-343 ◽

pp. 613-616

Author(s):

Xia Lu ◽

Li Ang Xing ◽

Pei Zhi Wang ◽

Jun Fu

Keyword(s):

Alkaline Phosphatase ◽

Titanium Implant ◽

Study Groups ◽

Porous Titanium ◽

Bone Sialoprotein ◽

Control Group ◽

Ha Coating ◽

Titanium Material ◽

Polymerase Chain

The aim of this study is to fabricate an implant framework for tissue engineering by sintering titanium beads and coating with hydroxyapatite and test its biocompatibility and bioactivity in vitro. The porous titanium with and without hydroxyapatite coating were involved in study groups. Osteoblastic proliferation, activity of alkaline phosphatase, mRNA of osteocalcin and bone sialoprotein were detected by MTT-assay, ALP test and real-time polymerase chain reaction respectively. The results indicated that the porous titanium material with/without HA coating could promote osteoblastic proliferation significantly contrast to the control group. However, only porous titanium with HA coating increased alkaline phosphatase, osteocalcin and bone sialoprotein gene expression apparently and had statistically differences with the other two groups. Abstract no. is TE-Po-044

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Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

Chemical Research in Chinese Universities ◽

10.1007/s40242-019-9101-x ◽

2019 ◽

Vol 35 (4) ◽

pp. 686-692 ◽

Cited By ~ 1

Author(s):

Mengna Duan ◽

Xiaofeng Wu ◽

Long Yuan ◽

Zhiying Zhang ◽

Yan Zhang ◽

...

Keyword(s):

Titanium Implant ◽

Porous Titanium ◽

Hydroxyapatite Coating ◽

In Vitro Bioactivity

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Preparation of Porous Titanium by a Novel Foaming Process and MG63 Cell Behavior In Vitro

Materials Science Forum ◽

10.4028/www.scientific.net/msf.610-613.1155 ◽

2009 ◽

Vol 610-613 ◽

pp. 1155-1159 ◽

Cited By ~ 1

Author(s):

Yue Jun Chen ◽

Bo Feng ◽

Jie Weng ◽

Shu Xin Qu ◽

Jian Xin Wang ◽

...

Keyword(s):

Alkali Treatment ◽

In Vitro Study ◽

Titanium Implant ◽

Mg63 Cell ◽

Porous Titanium ◽

Osteosarcoma Cell ◽

Foaming Process ◽

Human Osteosarcoma Cell ◽

Good Biocompatibility

A novel two-step foaming process has been developed to prepare the porous titanium implant. By using H2O2 and stearic acid as foaming reagent ordinally, a foamed structure with an open, interconnected pore morphology was obtained. The mechanical property was determined by compressive test. In vitro study was conducted to evaluate the ability of the porous titanium to support the growth and differentiation of Human osteosarcoma cell line MG63. The results show that the porous titanium has better interconnection compared to that obtained by traditional slurry foaming and its compressive strength and Young’s modulus were approximate 23.6 MPa and 2.1 GPa, respectively. Cell culture experiment results indicate that the porous titanium has good biocompatibility and acid-alkali treatment facilitates the adherence and proliferation of cells.

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Osteoblast and bone tissue response to surface modified zirconia and titanium implant materials

Dental Materials ◽

10.1016/j.dental.2013.04.003 ◽

2013 ◽

Vol 29 (7) ◽

pp. 763-776 ◽

Cited By ~ 52

Author(s):

Ralf J. Kohal ◽

Maria Bächle ◽

Wael Att ◽

Saad Chaar ◽

Brigitte Altmann ◽

...

Keyword(s):

Bone Tissue ◽

Titanium Implant ◽

Tissue Response ◽

Implant Materials ◽

Surface Modified

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In Vitro Mineralization and Cell Adhesion on Surface Modified Poly (2 - hydroxy ethyl methacrylate-co-methyl methacrylate

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.493 ◽

2006 ◽

Vol 309-311 ◽

pp. 493-496 ◽

Cited By ~ 3

Author(s):

G.S. Sailaja ◽

T.V. Kumari ◽

Yoshiyuki Yokogawa ◽

H.K. Varma

Keyword(s):

Cell Adhesion ◽

Calcium Phosphate ◽

Methyl Methacrylate ◽

Radical Copolymerization ◽

Sem Analysis ◽

Free Radical Copolymerization ◽

In Vitro Mineralization ◽

Atr Spectroscopy ◽

Surface Modified

Poly(2-hydroxyethyl methacrylate-co- methyl methacrylate) HM, was synthesized by free radical copolymerization, cross-linked with ethylene glycol dimethacrylate and phosphorylated. The phosphate coupling was ensured by ATR spectroscopy. The in vitro mineralization ability of the phosphorylated HM (designated as PHM) was investigated by studying the nucleation and growth of calcium phosphate on its surface by immersing in simulated body fluid (SBF) solution. The coating morphology was studied by SEM and the Ca/P ratio of the coating by EDX analysis. The cell adhesion behaviour of PHM was studied by seeding Human osteosarcoma (HOS) cells for one week followed by SEM analysis along with HM as control. It was observed that HOS cells exhibited biomineralization of calcium phosphate on the surface of HM as well as on PHM with a significantly higher amount on the surface of PHM as observed by von kossa staining method. The results show that PHM is capable of in vitro mineralization under simulated physiological condition, promotes cell adhesion by providing an excellent cell friendly surface and it exhibits biomineralization of calcium phosphate in presence of HOS cells.

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Understanding long-term silver release from surface modified porous titanium implants

Acta Biomaterialia ◽

10.1016/j.actbio.2017.05.048 ◽

2017 ◽

Vol 58 ◽

pp. 550-560 ◽

Cited By ~ 30

Author(s):

Anish Shivaram ◽

Susmita Bose ◽

Amit Bandyopadhyay

Keyword(s):

Porous Titanium ◽

Titanium Implants ◽

Silver Release ◽

Surface Modified

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Porous Titanium Associated with CaP Coating: In Vivo and In Vitro Osteogenic Performance

Materials Research ◽

10.1590/1980-5373-mr-2017-0557 ◽

2017 ◽

Vol 21 (2) ◽

Author(s):

Luana Marotta Reis de Vasconcellos ◽

Rodrigo Dias Nascimento ◽

Carlos Alberto Alves Cairo ◽

Daniel de Oliveira Leite ◽

Evelyn Luzia de Souza Santos ◽

...

Keyword(s):

Porous Titanium

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Retraction: In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Dental Materials Journal ◽

10.4012/dmj.2012-015-e ◽

2014 ◽

Vol 33 (1) ◽

pp. 148-148

Author(s):

Sonia SANTANDER ◽

Clara ALCAINE ◽

Jaber LYAHYAI ◽

Maria Angeles PÉREZ ◽

Clementina RODELLAR ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Titanium Alloy ◽

Human Mesenchymal Stem Cells ◽

Biomimetic Surface ◽

Surface Modified

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Surface Modification Of Polymers With Grafting And Coating Of Silane Hybrids And Their Bioactivity

MRS Proceedings ◽

10.1557/proc-576-377 ◽

1999 ◽

Vol 576 ◽

Cited By ~ 3

Author(s):

Masaaki Kubo ◽

Seisuke Takashima ◽

Kanji Tsuru ◽

Satoshi Hayakawa ◽

Akiyoshi Osaka ◽

...

Keyword(s):

Contact Angle ◽

High Density Polyethylene ◽

Body Fluid ◽

Chemical Species ◽

Hydrated Silica ◽

Bioactive Materials ◽

Poly Vinyl Chloride ◽

Essential Chemical ◽

Surface Modified

ABSTRACTHydrated silica rich Si-OH and Si-0- groups serve in a body environment as sites for nucleation of apatite, and are known as an essential chemical species for bioactive materials. Organic polymers having surface modified with the hydrated silica will show bioactivity: bone tissues grow toward the apatite layer and bond to materials. Thus MOPS-M (3-methacryloxypropyltrimethoxysilane) was grafted under emulsion polymerization procedure to high density polyethylene (HDPE), poly (vinyl chloride) (PVC) and polyamide (PA) substrates to examine in vitro deposition of apatite (bioactivity) after soaking in a simulated body fluid (Kokubo solution). Bioactivity was confirmed for the grafted PVC and PA substrates and discussed in terms of contact angle and relative amount of grafted silane molecules.

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