Fabrication and Bioactivity of Porous Titanium Implant

2007 ◽  
Vol 342-343 ◽  
pp. 613-616
Author(s):  
Xia Lu ◽  
Li Ang Xing ◽  
Pei Zhi Wang ◽  
Jun Fu
Keyword(s):  
Alkaline Phosphatase ◽  
Titanium Implant ◽  
Study Groups ◽  
Porous Titanium ◽  
Bone Sialoprotein ◽  
Control Group ◽  
Ha Coating ◽  
Titanium Material ◽  
Polymerase Chain

The aim of this study is to fabricate an implant framework for tissue engineering by sintering titanium beads and coating with hydroxyapatite and test its biocompatibility and bioactivity in vitro. The porous titanium with and without hydroxyapatite coating were involved in study groups. Osteoblastic proliferation, activity of alkaline phosphatase, mRNA of osteocalcin and bone sialoprotein were detected by MTT-assay, ALP test and real-time polymerase chain reaction respectively. The results indicated that the porous titanium material with/without HA coating could promote osteoblastic proliferation significantly contrast to the control group. However, only porous titanium with HA coating increased alkaline phosphatase, osteocalcin and bone sialoprotein gene expression apparently and had statistically differences with the other two groups. Abstract no. is TE-Po-044

scholarly journals Biological characterization of implant surfaces - in vitro study

2015 ◽  
Vol 44 (4) ◽  
pp. 195-199 ◽  
Author(s):  
Priscilla Barbosa Ferreira Soares ◽  
Camilla Christian Gomes Moura ◽  
Huberth Alexandre da Rocha Júnior ◽  
Paula Dechichi ◽  
Darceny Zanetta-Barbosa
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Viability ◽  
Total Protein ◽  
Cell Attachment ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Serum Total Protein ◽  
Trypan Blue Exclusion ◽  
Experimental Surface

<title>Abstract</title><sec><title>Objective</title><p>Evaluate the biological performance of titanium alloys grade IV under different surface treatments: sandblasting and double etching (Experimental surface 1; Exp1, NEODENT); surface with wettability increase (Experimental surface 2; Exp2, NEODENT) on response of preliminary differentiation and cell maturation.</p></sec><sec><title>Material and method</title><p>Immortalized osteoblast cells were plated on Exp1 and Exp2 titanium discs. The polystyrene plate surface without disc was used as control group (C). Cell viability was assessed by measuring mitochondrial activity (MTT) at 4 and 24 h (n = 5), cell attachment was performed using trypan blue exclusion within 4 hours (n = 5), serum total protein and alkaline phosphatase normalization was performed at 4, 7 and 14 days (n = 5). Data were analyzed using one-way ANOVA and Tukey test.</p></sec><sec><title>Result</title><p>The values of cell viability were: 4h: C– 0.32±0.01<sup>A</sup>; Exp1– 0.34±0.08<sup>A</sup>; Exp2– 0.29±0.03<sup>A</sup>. 24h: C– 0.43±0.02<sup>A</sup>; Exp1– 0.39±0.01<sup>A</sup>; Exp2– 0.37±0.03<sup>A</sup>. The cell adhesion counting was: C– 85±10<sup>A</sup>; Exp1- 35±5<sup>B</sup>; Exp2– 20±2<sup>B</sup>. The amounts of serum total protein were 4d: C– 40±2<sup>B</sup>; Exp1– 120±10<sup>A</sup>; Exp2– 130±20<sup>A</sup>. 7d: C– 38±2<sup>B</sup>; Exp1– 75±4<sup>A</sup>; Exp2– 70±6<sup>A</sup>. 14 d: C– 100±3<sup>A</sup>; Exp1– 130±5<sup>A</sup>; Exp2– 137±9<sup>A</sup>. The values of alkaline phosphatase normalization were: 4d: C– 2.0±0.1<sup>C</sup>; Exp1– 5.1±0.8<sup>B</sup>; Exp2– 9.8±2.0<sup>A</sup>. 7d: C– 1.0±0.01<sup>C</sup>; Exp1– 5.3±0.5<sup>A</sup>; Exp2– 3.0±0.3<sup>B</sup>. 14 d: C– 4.1±0.3<sup>A</sup>; Exp1– 4.4±0.8<sup>A</sup>; Exp2– 2.2±0.2<sup>B</sup>. Different letters related to statistical differences.</p></sec><sec><title>Conclusion</title><p>The surfaces tested exhibit different behavior at dosage of alkaline phosphatase normalization showing that the Exp2 is more associated with induction of cell differentiation process and that Exp1 is more related to the mineralization process.</p></sec>

Treatment with roscovitine and butyrolactone I prior to in vitro maturation alters blastocyst production

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 24-31 ◽  
Author(s):  
Rosiara Rosária Dias Maziero ◽  
Carlos Renato de Freitas Guaitolini ◽  
Daniela Martins Paschoal ◽  
André Maciel Crespilho ◽  
Bianca Andriolo Monteiro ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Study Groups ◽  
Embryo Cryopreservation ◽  
Control Group ◽  
Bovine Embryos ◽  
Nuclear Maturation ◽  
Oocyte Meiosis ◽  
Maturation Index ◽  
Bovine Oocytes

SummaryThis study evaluated the effects of oocyte meiosis inhibitors roscovitine (ROS) and butyrolactone I (BL-I) on in vitro production of bovine embryos. Bovine oocytes were maintained in pre in vitro maturation (pre-IVM) with 25 µM ROS or 100 µM BL-I for 24 h to delay meiosis and for 24 h in in vitro maturation (IVM). Following this treatment, the nuclear maturation index was evaluated. All embryos degenerated following this procedure. In the second set of experiments, oocytes were maintained for 6 or 12 h in pre-IVM with the following three treatments: ROS (25 µM or 12.5 µM), BL-I (100 µM or 50 µM) or a combination of both drugs (6.25 µM ROS and 12.5 µM BL-I). Oocytes were cultivated for 18 or 12 h in IVM. When a meiosis-inducing agent was used during pre-IVM for 24 h, more degenerated oocytes were observed at the end of the IVM period. This effect decreased when the meiotic blocking period was reduced to 6 or 12 h. No significant differences were observed in the blastocyst production rate of oocytes in pre-IVM for 6 h with ROS, BL-I, or ROS + BL-I compared with that of the control group (P > 0.05). However, inhibition of oocytes for 12 h resulted in decreased embryo production compared with that in the controls (P < 0.05). There was no difference in the post-vitrification embryo re-expansion rate between the study groups, showing that the meiotic inhibition for 6 or 12 h did not alter the embryo cryopreservation process.

scholarly journals Antimicrobial properties of antibiotic-loaded implants

2020 ◽  
Vol 102-B (6_Supple_A) ◽  
pp. 158-162
Author(s):  
Quentin Griseti ◽  
Christophe Jacquet ◽  
Pierre Sautet ◽  
Matthew P. Abdel ◽  
Sébastien Parratte ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Antimicrobial Properties ◽  
Porous Metal ◽  
Porous Titanium ◽  
Inhibitory Effect ◽  
Control Group ◽  
Local Antibiotics ◽  
Significant Difference ◽  
Vitro Analysis

Aims The aim of this study was to compare the ability of tantalum, 3D porous titanium, antibiotic-loaded bone cement, and smooth titanium alloy to inhibit staphylococci in an in vitro environment, based on the evaluation of the zone of inhibition (ZOI). The hypothesis was that there would be no significant difference in the inhibition of methicillin-sensitive or methicillin-resistant Staphylococcus aureus (MSSA/MRSA) between the two groups. Methods A total of 30 beads made of three different materials (tantalum/3D porous titanium and smooth titanium alloy) were bathed for one hour in a solution of 1 g vancomycin in 20 ml of sterile water for injection (bath concentration: 50 mg/mL). Ten 1 cm3 cylinders of antibiotic-loaded cement were also created by mixing standard surgical cement with 1 g of vancomycin in standardized sterile moulds. The cylinders were then placed on agar plates inoculated with MSSA and MRSA. The ZOIs were measured each day and the cylinders were transferred onto a new inoculated plate. Results For MSSA and MRSA, no inhibitory effect was found in the control group, and antibiotic-loaded smooth titanium alloy beads showed a short inhibitory effect until day 2. For MSSA, both tantalum and 3D porous titanium beads showed significantly larger mean ZOIs than cement beads (all p < 0.01) each day until day 7 for tantalum and until day 3 for 3D porous titanium. After six days, antibiotic-loaded cement had significantly larger mean ZOIs than the 3D porous titanium (p = 0.027), but no significant difference was found with tantalum (p = 0.082). For MRSA, both tantalum and 3D porous titanium beads had significantly larger mean ZOIs than antibiotic-loaded cement each day until day 6 for tantalum (all p < 0.01) and until day 3 for 3D porous titanium (all p < 0.04). Antibiotic-loaded cement had significantly larger mean ZOIs than tantalum and 3D porous titanium from day 7 to 9 (all p < 0.042). Conclusion These results show that porous metal implants can deliver local antibiotics over slightly varying time frames based on in vitro analysis. Cite this article: Bone Joint J 2020;102-B(6 Supple A):158–162.

The RPMI-1640 vitamin mixture promotes bovine blastocyst development in vitro and downregulates gene expression of TXNIP with epigenetic modification of associated histones

2017 ◽  
Vol 9 (1) ◽  
pp. 87-94 ◽  
Author(s):  
S. Ikeda ◽  
M. Sugimoto ◽  
S. Kume
Keyword(s):  
Polymerase Chain Reaction ◽  
Treated Group ◽  
Preimplantation Embryos ◽  
Control Group ◽  
Chain Reaction ◽  
Blastocyst Development ◽  
Polymerase Chain ◽  
Vitamin Mixture

Diverse environmental conditions surrounding preimplantation embryos, including available nutrients, affect their metabolism and development in both short- and long-term manner. Thioredoxin-interacting protein (TXNIP) is a possible marker for preimplantation stress that is implicated in in vitro fertilization- (IVF) induced long-term DOHaD effects. B vitamins, as participants in one-carbon metabolism, may affect preimplantation embryos by epigenetic alterations of metabolically and developmentally important genes. In vitro-produced bovine embryos were cultured with or without Roswell Park Memorial Institute 1640 vitamin mixture, containing B vitamins and B vitamin-like substances, from day 3 after IVF and we evaluated blastocyst development and TXNIP messenger RNA (mRNA) expression in the blastocysts by reverse transcription-quantitative polymerase chain reaction. The degree of trimethylation of histone H3 lysine 27 (H3K27me3) at TXNIP promoter was examined semi-quantitatively by chromatin immunoprecipitation polymerase chain reaction. Total H3K27me3 were also compared between the groups by Western blot analysis. The vitamin treatment significantly increased the rates of blastocyst development (P<0.05) and their hatching (P<0.001) from the zona pellucida by day 8. The mRNA expression of TXNIP was lower (P<0.01) in blastocysts in the vitamin-mixture-treated group concomitant with higher (P<0.05) level of H3K27me3 of its promoter compared with the control group. The total H3K27me3 in the vitamin-mixture-treated group was also higher (P<0.01) than that in the control group. The epigenetic control of genes related to important metabolic processes during the periconceptional period by nutritional conditions in utero and/or in vitro may have possible implication for the developmental programming during this period that may impact the welfare and production traits of farm animals.

Frequency of Y Chromosome Microdeletions in Azoospermic and Oligospermic Iranian Infertile Men

2020 ◽  
Author(s):  
Sepideh Gholami Yarahmadi ◽  
Saeid Morovvati ◽  
Monireh Raam ◽  
Ziba Morovvati
Keyword(s):  
Polymerase Chain Reaction ◽  
Y Chromosome ◽  
In Vitro Fertilisation ◽  
Control Group ◽  
Chain Reaction ◽  
Azoospermia Factor ◽  
Infertile Men ◽  
Y Chromosome Microdeletions ◽  
Polymerase Chain

Background and Aims: Azoospermia factor (AZF) region of the Ychromosome has several genes which are responsible for normal spermatogenesis. Microdeletions of these genes are associated with azoospermia and oligospermia. These microdeletions are too small to be detected by karyotyping. They can be easily identified using polymerase chain reaction. The aim of this study is to determine the frequencies of Ychromosome microdeletions in azoospermic and oligospermic Iranian infertile men and compare them with other studies in different ethnic groups. Materials and Methods: At first, karyotype analysis was performed in 80 infertile men and 30 healthy age-matched counterparts as control group using standard cytogenetic methods. Second, genomic DNA was extracted from all cases and genetic screening was conducted for Y chromosome microdeletions by multiplex polymerase chain reaction for AZF genes on both infertile and control men using 6 STS markers on the long arm of the Y chromosome. Results: Totally, 49 infertile men were azoospermic and 31 were oligospermic. Y-chromosome microdeletions in the AZFc region were detected in 4 of azoospermic patients. Y-chromosome microdeletions was not detected in any of the oligospermic patients and the control group. Conclusions: This finding recommends that genetic counseling and screening before starting assisted reproductive techniques such as in vitro fertilisation and intracytoplasmic sperm injection can prevent unnecessary treatment and transmission of genetic defects to offspring

scholarly journals Preferential usage of the bone-type leader sequence for the transcripts of liver/bone/kidney-type alkaline phosphatase gene in neutrophilic granulocytes

Blood ◽  
1994 ◽  
Vol 83 (4) ◽  
pp. 1093-1101 ◽  
Author(s):  
N Sato ◽  
Y Takahashi ◽  
S Asano
Keyword(s):  
Alkaline Phosphatase ◽  
Retinoic Acid ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Granulocyte Colony Stimulating Factor ◽  
Chromosomal Structure ◽  
Polymerase Chain ◽  
Stimulating Factor ◽  
In Vitro Treatment

Abstract Alkaline phosphatase in neutrophils (NAP) is a product of the liver/bone/kidney-type alkaline phosphatase gene, the chromosomal structure of which has recently been analyzed. The gene has two alternative leader sequences resulting in the two types of mRNA (liver- type and bone-type mRNAs), which suggests that the expression of the gene is regulated by independent promoters. To determine the mechanism underlying NAP induction, it is essential to know which type of the mRNAs is dominant in neutrophils. We adopted quantitative polymerase chain reaction method to determine the relative amount of bone-type mRNA in neutrophils. The bone-type mRNA was found to be at least 5 times more than the liver-type mRNA. mRNA of NAP is known to be induced by in vitro treatment of the cells with granulocyte colony-stimulating factor (G-CSF), which is enhanced by a simultaneous addition of retinoic acid. In neutrophils treated with G-CSF, the bone-type mRNA was at least 6 times more than the liver-type mRNA. In neutrophils treated by both G-CSF and retinoic acid, the bone-type mRNA was at least 22 times more than the liver-type mRNA. The results show that the bone-type mRNA is predominantly transcribed in peripheral neutrophils and in neutrophils cultured in vitro.

Fabrication and In vitro Bioactivity of Robust Hydroxyapatite Coating on Porous Titanium Implant

2019 ◽  
Vol 35 (4) ◽  
pp. 686-692 ◽  
Author(s):  
Mengna Duan ◽  
Xiaofeng Wu ◽  
Long Yuan ◽  
Zhiying Zhang ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Titanium Implant ◽  
Porous Titanium ◽  
Hydroxyapatite Coating ◽  
In Vitro Bioactivity

Nanospike surface-modified bionic porous titanium implant and in vitro osteogenic performance

2017 ◽  
Vol 27 (8) ◽  
pp. 1815-1821 ◽  
Author(s):  
Guo-hui WANG ◽  
Hua FU ◽  
Ke-chao ZHOU ◽  
Yan-zhong ZHAO ◽  
Shai-hong ZHU
Keyword(s):  
Titanium Implant ◽  
Porous Titanium ◽  
Surface Modified

scholarly journals Natural fruit peels as teeth whitening agents on primary teeth an in vitro study

2021 ◽  
Vol 12 (2) ◽  
pp. 232-235
Author(s):  
Archana P Betur ◽  
Raju O S ◽  
Roopa K B
Keyword(s):  
Orange Peel ◽  
Study Groups ◽  
Enamel Surface ◽  
Banana Peel ◽  
Staining Procedure ◽  
Control Group ◽  
Standard Group ◽  
Deciduous Dentition ◽  
Anterior Teeth

Context: Dental Bleaching or tooth whitening represents a conservative cosmetic approach even for children and adolescents, for whom treatment may be readily accomplished without permanent alteration to the enamel surface thus limiting the need for lifelong maintenance. Aims: The aim of this study was to compare the efficacy of banana peel and orange peel as teeth   whitening agents in deciduous dentition. Methods and Material: Twenty extracted primary anterior teeth were used in this study and each crown was sectioned mesiodistally to obtain 40 samples which were flattened to create a standardized enamel surface. After the staining procedure, 40 samples were allocated into 4 groups, i.e., 10 samples per group, the standard group (35% Hydrogen peroxide), the control group (distilled water), banana peel and orange peel. The samples of each group were treated using the respective agents with standardized pressure and time. Results: The tooth shades were assessed using Digital Spectrophotometer at baseline, 1 week of staining, 1 week of bleaching and 1 week post bleaching. All the results were statistically analysed with Post Hoc Tukey’s test. Both the study groups showed significant changes but, banana peel showed better efficacy than orange peel. Conclusions: Banana peel and orange peel can be considered for teeth whitening in deciduous dentition.

scholarly journals Infiltration of Rhodamin B into three materials used as cervical barrier

2014 ◽  
Vol 62 (3) ◽  
pp. 235-242
Author(s):  
Grazielle Morais de SOUZA ◽  
Luis Cardoso RASQUIN ◽  
Fabíola Bastos de CARVALHO
Keyword(s):  
Study Groups ◽  
Control Group ◽  
Sodium Perborate ◽  
Filling Material ◽  
Significant Difference ◽  
Materials Used ◽  
Level Of Significance ◽  
Material Setting ◽  
Rhodamin B

OBJECTIVE: To evaluate in vitro the sealing ability of three materials used as cervical barrier for internal dental bleaching. METHODS: Thirty-three canines were selected, and after biomechanical root canal preparation, the canals were filled by lateral condensation technique. After the filling material setting reaction, the teeth were randomly divided into three groups of ten teeth each, according to the following materials for constructing the cervical barrier: X Temp LC; Vitro Fil; Coltosol. The control group consisted of three teeth, without placement of the cervical cap. Sodium perborate /distilled water associated with 2% Rhodamin B were used as bleaching substance. After placing the bleaching agent in the pulp chamber, access cavities were sealed with temporary cement IRM and samples were stored at 37 ° for 7 days. After this period, the samples were cleaved longitudinally, and leakage was evaluated by the visual method. The data were submitted to Pearson test with a 5% level of significance. RESULTS: No statistically significant difference was found between the study groups, however, the largest number of specimens with a lower degree of leakage were found in the Coltosol group. CONCLUSION: All the materials evaluated for making the cervical cap allowed some level of leakage.

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