Cadmium induces the production of high molecular weight heparan sulfate proteoglycan molecules in cultured vascular endothelial cells

1997 ◽  
Vol 3 (3) ◽  
pp. 187-194 ◽  
Author(s):  
Susumu Ohkawara ◽  
Chika Yamamoto ◽  
Yasuyuki Fujiwara ◽  
Michiko Sakamoto ◽  
Toshiyuki Kaji
Keyword(s):  
Molecular Weight ◽  
Endothelial Cells ◽  
Heparan Sulfate ◽  
High Molecular Weight ◽  
Vascular Endothelial Cells ◽  
Heparan Sulfate Proteoglycan ◽  
Vascular Endothelial ◽  
Sulfate Proteoglycan

scholarly journals Copper(II) Bis(diethyldithiocarbamate) Induces the Expression of Syndecan-4, a Transmembrane Heparan Sulfate Proteoglycan, via p38 MAPK Activation in Vascular Endothelial Cells

2018 ◽  
Vol 19 (11) ◽  
pp. 3302 ◽  
Author(s):  
Takato Hara ◽  
Hiroko Tatsuishi ◽  
Tomomi Banno ◽  
Tomoya Fujie ◽  
Chika Yamamoto ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Heparan Sulfate ◽  
Blood Coagulation ◽  
P38 Mapk ◽  
Vascular Endothelial Cells ◽  
Heparan Sulfate Proteoglycan ◽  
Mitogen Activated Protein Kinase ◽  
Dependent Manner ◽  
Vascular Endothelial ◽  
Sulfate Proteoglycan

Proteoglycans synthesized by vascular endothelial cells are important for regulating cell function and the blood coagulation-fibrinolytic system. Since we recently reported that copper(II) bis(diethyldithiocarbamate) (Cu(edtc)2) modulates the expression of some molecules involving the antioxidant and blood coagulation systems, we hypothesized that Cu(edtc)2 may regulate the expression of proteoglycans and examined this hypothesis using a bovine aortic endothelial cell culture system. The experiments showed that Cu(edtc)2 induced the expression of syndecan-4, a transmembrane heparan sulfate proteoglycan, in a dose- and time-dependent manner. This induction required the whole structure of Cu(edtc)2—the specific combination of intramolecular copper and a diethyldithiocarbamate structure—as the ligand. Additionally, the syndecan-4 induction by Cu(edtc)2 depended on the activation of p38 mitogen-activated protein kinase (MAPK) but not the Smad2/3, NF-E2-related factor2 (Nrf2), or epidermal growth factor receptor (EGFR) pathways. p38 MAPK may be a key molecule for inducing the expression of syndecan-4 in vascular endothelial cells.

High molecular weight adiponectin activates AMPK and suppresses cytokine-induced NF-κB activation in vascular endothelial cells

FEBS Letters ◽  
2008 ◽  
Vol 582 (12) ◽  
pp. 1719-1724 ◽  
Author(s):  
Yoshiyuki Hattori ◽  
Yasuko Nakano ◽  
Sachiko Hattori ◽  
Atsuko Tomizawa ◽  
Kouichi Inukai ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Endothelial Cells ◽  
High Molecular Weight ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
High Molecular Weight Adiponectin

scholarly journals Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells

2020 ◽  
Vol 21 (10) ◽  
pp. 3698 ◽  
Author(s):  
Takato Hara ◽  
Shiori Yabushita ◽  
Chika Yamamoto ◽  
Toshiyuki Kaji
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Heparan Sulfate ◽  
Fibroblast Growth Factor ◽  
Cell Density ◽  
Vascular Endothelial Cells ◽  
Fibroblast Growth Factor 2 ◽  
Vascular Endothelial ◽  
Fibroblast Growth ◽  
Density Dependent

Syndecan-4 is a member of the syndecan family of transmembrane heparan sulfate proteoglycans, and is involved in cell protection, proliferation, and the blood coagulation-fibrinolytic system in vascular endothelial cells. Heparan sulfate chains enable fibroblast growth factor-2 (FGF-2) to form a complex with its receptor and to transduce the cell growth signal. In the present study, bovine aortic endothelial cells were cultured, and the intracellular signal pathways that mediate the regulation of syndecan-4 expression in dense and sparse cultures by FGF-2 were analyzed. We demonstrated the cell density-dependent differential regulation of syndecan-4 expression. Specifically, we found that FGF-2 upregulated the synthesis of syndecan-4 in vascular endothelial cells via the MEK1/2-ERK1/2 pathway in dense cell cultures, with only a transcriptional induction of syndecan-4 at a low cell density via the Akt pathway. This study highlights a critical mechanism underlying the regulation of endothelial cell functions by proteoglycans.

The binding of heparin to the extracellular matrix of endothelial cells up-regulates the synthesis of an antithrombotic heparan sulfate proteoglycan

2008 ◽  
Vol 217 (2) ◽  
pp. 328-337 ◽  
Author(s):  
Edvaldo S. Trindade ◽  
Constance Oliver ◽  
Maria C. Jamur ◽  
Hugo A.O. Rocha ◽  
Célia R.C. Franco ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Heparan Sulfate ◽  
Heparan Sulfate Proteoglycan ◽  
Sulfate Proteoglycan
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