HSV-1 DNA stability in LB (lysis buffer) vs. VTM (virus transport medium) at room temperature

This protocol is designed for water collection from Niskin bottles and filtration at sea using reusable filter cups. Aim: to collect and filter 2.5 L of water at each depth from each CTD cast and preserved the filter at room temperature in lysis buffer

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Comparison of Respiratory Virus Detection Rates for Infants and Toddlers by Use of Flocked Swabs, Saline Aspirates, and Saline Aspirates Mixed in Universal Transport Medium for Room Temperature Storage and Shipping

Journal of Clinical Microbiology ◽

10.1128/jcm.00714-08 ◽

2008 ◽

Vol 46 (7) ◽

pp. 2374-2376 ◽

Cited By ~ 35

Author(s):

P. Walsh ◽

C. L. Overmyer ◽

K. Pham ◽

S. Michaelson ◽

L. Gofman ◽

...

Keyword(s):

Room Temperature ◽

Respiratory Virus ◽

Virus Detection ◽

Infants And Toddlers ◽

Detection Rates ◽

Room Temperature Storage ◽

Transport Medium ◽

Temperature Storage

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Mouse sperm delivery via cauda epididymis without transport medium

10.7287/peerj.preprints.2542v1 ◽

2016 ◽

Author(s):

Man-Xi Jiang ◽

Mei-Shan Wang ◽

Xiang-Hong Ou ◽

Xue-Jin Chen ◽

Yan Zhu

Keyword(s):

Embryo Development ◽

Sperm Motility ◽

Room Temperature ◽

Sperm Quality ◽

Mouse Sperm ◽

Spermatozoa Motility ◽

Transport Medium ◽

Fertilization Rates ◽

Progressive Motility

This study was aimed to investigate the effects of room temperature (RT, 20-25 oC) and absence of medium during cauda epididymis transport on spermatozoa quality, fertility and embryo development. In the first experiment, fresh sperm from one side of cauda epididymis was used for in vitro fertilization, and another side was delivered at RT or 4-8 oC either with or without M2. In the second experiment, each side of cauda epididymis obtained from the same mouse was individually delivered at RT or 4 oC with or without M2. Finally, sperm motility, progressive motility scores and fertility of fresh spermatozoa or those from transported cauda epididymis, and IVF embryo development were evaluated. Progressive motility scores and fertilization rates were higher in fresh spermatozoa than transported sperm; sperm motility of transported cauda epididymis at 4-8 oC was comparable to fresh spermatozoa, but spermatozoa motility of transported cauda epididymis at RT was inferior to fresh spermatozoa. Spermatozoa motillty of transported cauda epididymis at 4-8 oC with transport medium was much higher than that without transport medium; absence of transport medium did not affect sperm motility of transported cauda epididymis at 4-8 oC but affected sperm motility of transported cauda epididymis at RT. Sperm quality from transported cauda epididymis can be efficiently kept at 4-8 oC, and cauda epididymis transport at 4-8 oC without M2 is more beneficial on keeping their fertility. Moreover cauda epididymis transport at RT without medium could sufficiently produce embryos for obtainning live offsprings.

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Quality of Cary-Blair Transport Medium After Aging Nineteen Months

Journal of Clinical Microbiology ◽

10.1128/jcm.8.5.616-617.1978 ◽

1978 ◽

Vol 8 (5) ◽

pp. 616-617

Author(s):

George K. Morris ◽

Jean Heck

Keyword(s):

Room Temperature ◽

Transport Medium

Cary-Blair medium retains its ability to serve as a transport medium after 19 months of aging at room temperature. This eliminates the necessity for the frequent replacement of the stored supplies of this medium.

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The testing and modification of a commercially available transport medium for the transportation of pure cultures of Haemophilus paragallinarum for serotyping

Onderstepoort Journal of Veterinary Research ◽

10.4102/ojvr.v71i2.270 ◽

2004 ◽

Vol 71 (2) ◽

Cited By ~ 2

Author(s):

R.R. Bragg ◽

P. Jansen van Rensburg ◽

E. Van Heerden ◽

J. Albertyn

Keyword(s):

Causative Agent ◽

Room Temperature ◽

Specific Growth ◽

Growth Conditions ◽

Complete Control ◽

Transport Medium ◽

Infectious Coryza ◽

Causative Agents ◽

Pure Cultures

Haemophilus paragallinarum, the causative agent of infectious coryza in poultry, is an extremely fastidious organism requiring specific growth conditions for isolation. For complete control of the disease in regions where more that one of the serovars of the different serogroups occurs, it is essential that the bacterium causing the problem be isolated and serotyped. This work describes the modification and testing of transport media, which will ensure the survival of the causative agents in suspected infectious coryza cases for transport to a laboratory where the bacterium can be isolated and serotyped. The various transport media used are based on commercially available Amies Transport Medium supplemented with the different supplements used for the growth of H. paragallinarum. It was established that the bacterium remains viable for up to 18 days in Amies Transport Medium containing all the supplements when stored at 4 oC or 37 oC. At room temperature or 25 oC, there was no difference in the survival of H. paragallinarum in commercial Amies Transport Medium (without charcoal) and Amies Transport Medium with supplements.

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Reliable detection, sequencing, and transfection of foot-and-mouth disease virus RNA from badly preserved vesicular epithelium

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638719870859 ◽

2019 ◽

Vol 31 (5) ◽

pp. 778-782 ◽

Cited By ~ 3

Author(s):

Veronika Dill ◽

Michael Eschbaumer

Keyword(s):

Disease Virus ◽

Room Temperature ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Transport Medium ◽

Virus Rna ◽

Mouth Disease Virus ◽

Reliable Detection ◽

Foot And Mouth ◽

Capsid Protein Vp1

Epithelium or fluid from vesicular lesions are the preferred samples to confirm foot-and-mouth disease virus (FMDV) infection in livestock. A pH-neutral buffered transport medium is recommended for optimal preservation of epithelial samples, but may not be necessary for all circumstances based on the results of our study. Pieces of epithelium were collected from FMDV-infected cattle (isolates O/FRA/1/2001 and A/IRN/22/2015) and stored at room temperature in sealed tubes without any liquid or preservatives. Using RNA extracted from the severely decayed epithelium up to 3 wk after collection, FMDV was successfully detected by RT-rtPCR, and the viral strain was identified by sequencing of capsid protein VP1. Direct isolation of the virus in cell culture was only possible for vesicular material stored for up to 2–5 d, depending on the serotype, but, for both serotypes, infectious virus was recovered by transfection of RNA extracted from epithelium after 3 wk of storage at room temperature. Specialized transport medium will give optimal results, particularly for low-titer samples, but is not required for the reliable detection and characterization of FMDV in highly positive vesicular epithelium by molecular methods.

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