Vaccination of guinea pigs with DNA encoding Ag85A by gene gun bombardment

Tuberculosis ◽  
2003 ◽  
Vol 83 (6) ◽  
pp. 331-337 ◽  
Author(s):  
I Sugawara ◽  
H Yamada ◽  
T Udagawa ◽  
K Huygen
Keyword(s):  
Guinea Pigs ◽  
Gene Gun ◽  
Dna Encoding

scholarly journals Lack of Protection in Mice and Necrotizing Bronchointerstitial Pneumonia with Bronchiolitis in Guinea Pigs Immunized with Vaccines Directed against the hsp60 Molecule ofMycobacterium tuberculosis

2000 ◽  
Vol 68 (6) ◽  
pp. 3674-3679 ◽  
Author(s):  
Oliver C. Turner ◽  
Alan D. Roberts ◽  
Anthony A. Frank ◽  
Susan W. Phalen ◽  
David M. McMurray ◽  
...  
Keyword(s):  
Heat Shock ◽  
Guinea Pigs ◽  
Lung Damage ◽  
Dna Encoding ◽  
Protein Antigens ◽  
Plasmid Vaccine ◽  
Potential Vaccine ◽  
Guinea Pig Model ◽  
Shock Proteins ◽  
Severe Lung

ABSTRACT In this study, the hsp60 and hsp70 heat shock protein antigens ofMycobacterium tuberculosis were tested as potential vaccine candidates, using purified recombinant protein antigens or antigens encoded in the form of a DNA plasmid vaccine. Guinea pigs vaccinated with a mixture of the two proteins showed no evidence of resistance to low-dose aerosol challenge infection and quickly developed severe lung damage characterized by necrotizing bronchointerstitial pneumonia and bronchiolitis. As a result, we turned instead to a DNA vaccination approach using a plasmid encoding the hsp60 antigen of M. tuberculosis. Although immunogenic in mice, vaccination with plasmid DNA encoding hsp60 was not protective in that model or in the guinea pig model and again gave rise to similar severe lung damage. This study seriously questions the safety of vaccines against tuberculosis that target highly conserved heat shock proteins.

scholarly journals Dermal dendritic cells sensitized with plasmid DNA encoding immunostimulatory sequence by gene gun efficiently prime murine HIV-1-specific CD8+ cytotoxic T lymphocytes

2004 ◽  
Vol 25 (2) ◽  
pp. 83-91
Author(s):  
Chizuno HIDAKA ◽  
Yoshihiko NOROSE ◽  
Yohko NAKAGAWA ◽  
Masumi SHIMIZU ◽  
Megumi TAKAHASHI ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Plasmid Dna ◽  
Gene Gun ◽  
Dna Encoding ◽  
Hiv 1

scholarly journals Coadministration of DNA Encoding Interleukin-6 and Hemagglutinin Confers Protection from Influenza Virus Challenge in Mice

1998 ◽  
Vol 72 (2) ◽  
pp. 1704-1708 ◽  
Author(s):  
Diane L. Larsen ◽  
Naomi Dybdahl-Sissoko ◽  
Martha W. McGregor ◽  
Robert Drape ◽  
Veronica Neumann ◽  
...  
Keyword(s):  
Immune Responses ◽  
Interleukin 6 ◽  
Influenza A ◽  
Dna Vaccination ◽  
Gene Gun ◽  
Dna Encoding ◽  
Challenge Virus ◽  
Virus Challenge ◽  
Equine Influenza ◽  
Homologous Challenge

ABSTRACT This study was conducted to investigate whether Accell gene gun coadministration of DNA encoding human interleukin-6 (IL-6) would enhance protective immune responses in mice to an equine influenza A virus hemagglutinin (HA) DNA vaccine. Mice that received HA DNA alone exhibited accelerated clearance of homologous challenge virus but were not protected from infection. In contrast, mice that received both HA and IL-6 DNA had no detectable virus in their lungs after challenge. These results strongly support the use of IL-6 as a cytokine adjuvant in DNA vaccination.

scholarly journals Vaccination of Guinea Pigs with DNA Encoding the Mycobacterial Antigen MPB83 Influences Pulmonary Pathology but Not Hematogenous Spread following Aerogenic Infection with Mycobacterium bovis

2002 ◽  
Vol 70 (4) ◽  
pp. 2159-2165 ◽  
Author(s):  
Mark A. Chambers ◽  
Ann Williams ◽  
Graham Hatch ◽  
Dolores Gavier-Widén ◽  
Graham Hall ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Guinea Pigs ◽  
Vaccination Strategy ◽  
Control Program ◽  
Dna Vaccination ◽  
Mycobacterial Antigen ◽  
Lung Pathology ◽  
Dna Encoding ◽  
Hematogenous Spread ◽  
Bcg Vaccination

ABSTRACT Protection of cattle against bovine tuberculosis by vaccination could be an important control strategy in countries where there is persistent Mycobacterium bovis infection in wildlife and in developing countries where it is not economical to implement a tuberculin test and slaughter control program. The main aim of such a vaccination strategy would be to reduce transmission of infection by reducing the lung pathology caused by infection and preventing seeding of the organism to organs from which M. bovis could be excreted. Recent reports of successful DNA vaccination against Mycobacterium tuberculosis in small-animal models have suggested that DNA vaccines act by reducing lung pathology without sensitizing animals to tuberculin testing. We therefore evaluated the ability of vaccines consisting of DNA encoding the mycobacterial antigens MPB83 and 85A to reduce lung pathology and prevent hematogenous spread in guinea pigs challenged with a low dose of aerosolized M. bovis. Vaccination with MPB83 DNA reduced the severity of pulmonary lesions, as assessed by histopathology, and resembled M. bovis BCG vaccination in this respect. However, unlike BCG vaccination, MPB83 DNA vaccination did not protect challenged guinea pigs from hematogenous spread of organisms to the spleen. In contrast, vaccination with antigen 85A DNA, a promising DNA vaccine for human tuberculosis, had no measurable protective effect against infection with M. bovis.

Plasmid DNA could be delivered into Eimeria maxima unsporulated oocyst with gene gun system

2012 ◽  
Vol 60 (4) ◽  
pp. 431-440 ◽  
Author(s):  
Jianan Li ◽  
Jun Zou ◽  
Guangwen Yin ◽  
Xianyong Liu ◽  
Xun Suo
Keyword(s):  
Plasmid Dna ◽  
Genomic Dna ◽  
Fluorescent Protein ◽  
Genetic Manipulation ◽  
Yellow Fluorescent Protein ◽  
Helium Pressure ◽  
Gene Gun ◽  
Dna Encoding ◽  
Enhanced Yellow Fluorescent Protein ◽  
Eimeria Maxima

Eimerian coccidia are the most common parasitic organisms infecting chickens. The feasibility of genetic manipulation of these parasites via electroporation is proven, but this method is cumbersome and time consuming. Here we report our endeavour to develop a rapid and simple transfection method by gene gun. Tungsten particles coated with plasmid DNA encoding enhanced yellow fluorescent protein (EYFP) were used for the bombardment of Eimeria maxima unsporulated oocysts. Seven Mpa (1015 psi) helium pressure, 65 mm target distance and −0.098 Mpa (24.8″ Hg) chamber vacuum were the optimised parameters for bombardment. After sporulation, the bombarded oocysts were inoculated into chickens, and the progeny oocysts were checked under fluorescent microscope and subjected to genomic DNA extraction, which was used either for polymerase chain reaction (PCR) amplification or plasmid rescue assay. Although the expression of EYFP was not observed, the gene was amplified from both genomic DNA and the rescued plasmid, suggesting that the plasmid DNA existed in the form of episome. These results are encouraging for the genetic processing of the sporogony stage of eimerian parasites.

Investigation of the relaxant effects of propofol on ovalbumin-induced asthma in guinea pigs

2006 ◽  
pp. 1
Author(s):  
I. Bagcivan ◽  
O. Cevit ◽  
M. K. Yildirim ◽  
S. Gursoy ◽  
S. Yildirim ◽  
...  
Keyword(s):  
Guinea Pigs

Hepatocyte Alterations in Guinea Pigs FED Polychlorinated Biphenyls (PCBs), Dibenzodioxins (PCDD), AND DIBENZOFURANS (PCDF)

1982 ◽  
Vol 40 ◽  
pp. 56-57
Author(s):  
J. N. Turner ◽  
D. N. Collins
Keyword(s):  
Guinea Pigs ◽  
Room Temperature ◽  
Dose Group ◽  
Methyl Cellulose ◽  
Uranyl Acetate ◽  
Target Organ ◽  
Office Building ◽  
Lead Citrate ◽  
Control Groups ◽  
Cooling Fluid

A fire involving an electric service transformer and its cooling fluid, a mixture of PCBs and chlorinated benzenes, contaminated an office building with a fine soot. Chemical analysis showed PCDDs and PCDFs including the highly toxic tetra isomers. Guinea pigs were chosen as an experimental animal to test the soot's toxicity because of their sensitivity to these compounds, and the liver was examined because it is a target organ. The soot was suspended in 0.75% methyl cellulose and administered in a single dose by gavage at levels of 1,10,100, and 500mgm soot/kgm body weight. Each dose group was composed of 6 males and 6 females. Control groups included 12 (6 male, 6 female) animals fed activated carbon in methyl cellulose, 6 males fed methyl cellulose, and 16 males and 10 females untreated. The guinea pigs were sacrificed at 42 days by suffocation in CO2. Liver samples were immediately immersed and minced in 2% gluteraldehyde in cacadylate buffer at pH 7.4 and 4°C. After overnight fixation, samples were postfixed in 1% OsO4 in cacodylate for 1 hr at room temperature, embedded in epon, sectioned and stained with uranyl acetate and lead citrate.

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

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